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Method for preparing reduction-sensitive medicine nano-agent for diagnoses and treatment through single-walled carbon nanotubes modified through hyaluronic acid and application of method

A single-walled carbon nanotube, hyaluronic acid modification technology, applied in the field of medicine, can solve the problems of low relaxation efficiency, poor tumor targeting, large toxic and side effects, and achieve huge economic and social benefits and good biocompatibility. , Good tumor targeting effect

Inactive Publication Date: 2015-09-30
ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In view of the above situation, in order to overcome the defects of the prior art, the purpose of the present invention is to provide a method and application of hyaluronic acid modified single-walled carbon nanotubes for preparing reduction-sensitive drug nano-agents for diagnosis and treatment, which can effectively solve the problem of anticancer drugs. Doxorubicin has poor tumor targeting, large toxic and side effects, poor targeting, low relaxation efficiency, and short half-life

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  • Method for preparing reduction-sensitive medicine nano-agent for diagnoses and treatment through single-walled carbon nanotubes modified through hyaluronic acid and application of method

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Embodiment 1

[0027] In concrete implementation, the present invention is realized by the following steps:

[0028] (1) Carboxylated carbon tubes (i.e. carboxylated carbon nanotubes) connected to ammoniated hyaluronic acid (HA-SWCNTs):

[0029] Weigh 50 mg of carboxylated carbon tubes and dissolve them in 20 ml of formamide, probe in an ice bath for over 25 minutes to form a carboxylated carbon tube solution; weigh ammoniated hyaluronic acid (HA-NH 2 ) 80 mg was dissolved in 10 ml of formamide to form an ammoniated hyaluronic acid solution; EDC (1-ethyl-(3-dimethylaminopropyl) carbodiimide) 160 mg, NHS (hydroxysuccinimide Amine) 90 mg, vortex dissolved with 2.5ml formamide, added to the carboxylated carbon tube solution, stirred at room temperature for 15 min to form a carboxylated carbon tube mixed solution; added 150 μl of triethylamine to the ammoniated hyaluronic acid solution, and then Quickly drop the mixed solution of ammoniated hyaluronic acid and triethylamine into the mixed solut...

Embodiment 2

[0040] In specific implementation, the present invention can also be realized by the following steps:

[0041] (1) Carboxylated carbon tubes (i.e. carboxylated carbon nanotubes) connected to ammoniated hyaluronic acid (HA-SWCNTs):

[0042] Weigh 60 mg of carboxylated carbon tubes and dissolve them in 20 ml of formamide, probe in an ice bath for over 30 minutes to form a carboxylated carbon tube solution; weigh ammoniated hyaluronic acid (HA-NH 2 ) 96 mg was dissolved in 10 ml of formamide to form an ammoniated hyaluronic acid solution; EDC (1-ethyl-(3-dimethylaminopropyl) carbodiimide) 160 mg, NHS (hydroxysuccinimide Amine) 108 mg, vortex dissolved with 3ml formamide, added to the carboxylated carbon tube solution, stirred at room temperature for 15 minutes to form a carboxylated carbon tube mixed solution; added 180 μl of triethylamine to the ammoniated hyaluronic acid solution, and then The mixed solution of ammoniated hyaluronic acid and triethylamine was quickly added dro...

Embodiment 3

[0053] In concrete implementation, the present invention is realized by the following steps:

[0054] (1) Carboxylated carbon tubes (i.e. carboxylated carbon nanotubes) connected to ammoniated hyaluronic acid (HA-SWCNTs):

[0055] Weigh 70mg of carboxylated carbon tubes and dissolve them in 28ml N,N-dimethylformamide, probe in an ice bath for over 35min to form a carboxylated carbon tube solution; weigh ammoniated hyaluronic acid (HA-NH 2 )112mg was dissolved in 14ml of N,N-dimethylformamide to form an ammoniated hyaluronic acid solution; EDC (1-ethyl-(3-dimethylaminopropyl) carbodiimide) 224mg , NHS (hydroxysuccinimide) 126mg, vortex dissolved with 3.5ml N,N-dimethylformamide, added to the carboxylated carbon tube solution, and stirred at room temperature for 18min to form a carboxylated carbon tube mixed solution; Add 210 μl of triethylamine to the ammoniated hyaluronic acid solution, then quickly drop the mixed solution of ammoniated hyaluronic acid and triethylamine into ...

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Abstract

The invention relates to a method for preparing a reduction-sensitive medicine nano-agent for diagnoses and treatment through single-walled carbon nanotubes modified through hyaluronic acid and application of the method. The method can effectively solve the problems that tumor targeting of the anti-cancer drug adriamycin is poor, toxic and side effects are large, targeting is poor, relaxivity is low and the half-life period is short. According to a transport system, the single-walled carbon nanotubes modified through the hyaluronic acid are connected with the adriamycin through 3,3'-dithio-thiodipropionic acid serving as a joint arm, then gadolinium chloride is adsorbed to the single-walled carbon nanotubes, and the single-walled carbon nanotubes modified through the hyaluronic acid are formed through dispersion of a surface active agent. The reduction-sensitive medicine nano-agent used for diagnoses and treatment has the advantages that biocompatibility and tumor targeting are good, thermal therapy and chemotherapy are combined, and diagnosis and treatment are combined, is an innovation of tumor diagnosis and treatment medicine, and is large in economic and social benefit.

Description

technical field [0001] The invention relates to medicine, in particular to a method and application of hyaluronic acid (HA)-modified single-walled carbon nanotubes (SWCNTs) for preparing reduction-sensitive drug nano-agents for diagnosis and treatment. Background technique [0002] Malignant tumors seriously threaten human health and life safety, and its treatment has become the focus of attention. According to epidemiological surveys in my country, the mortality rate of malignant tumors ranks first among all deadly diseases in both urban and rural areas. Doxorubicin hydrochloride (Doxorubicin, DOX) is a broad-spectrum anti-tumor chemotherapy drug that can kill tumor cells in various growth cycles. It is mainly used clinically to treat superficial solid tumors such as breast cancer, prostate cancer, and sarcoma. Currently, doxorubicin hydrochloride is in the form of an injection, which causes high toxicity to the hematopoietic system and the heart due to the lack of targetin...

Claims

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Application Information

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IPC IPC(8): A61K49/12A61K49/18A61K9/14A61K47/48A61K31/704A61P35/00
Inventor 侯琳杨晓敏张振中王用超任俊晓石宇洋冯倩华袁玉洁单晓宁张媛媛
Owner ZHENGZHOU UNIV
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