Bacillus strain for controlling soybean root rot and Bacillus microbial inoculant thereof
A technology for soybean root rot and bacillus, which is applied in the field of microorganisms to achieve the effect of saving expenses and increasing income
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Embodiment 1
[0027] Physiological and biochemical tests of strains and identification of 16S rDNA molecules
[0028] Such as figure 1 As shown, the bacillus strain JSCX-1 is activated and cultivated on the NA plate at a temperature of 29-31 ° C, NA medium: 3 g of beef extract (Beef Extract), 10 g of peptone (Peptone), sodium chloride (NaCl) 5g, 15g of agar powder (Agar), pH7.0.
[0029] After 20-24 hours of culture, it was found that the single colony of JSCX-1 was nearly round and milky white, and the long-term culture colony was brownish yellow, with neat edges, opaque, and slightly wrinkled surface. Gram staining of the strain JSCX-1 was purple, which was positive; the spores were green in the spore staining, and the cells were red. The result of the aerobic test of the strain JSCX-1 was that it grew along the surface of the culture medium, which indicated that the strain JSCX-1 was an aerobic bacterium.
[0030] Physiological and biochemical tests are oxidase test, hydrogen peroxide...
Embodiment 2
[0035] The preparation method steps of Phytophthora soybean zoospore suspension are as follows:
[0036] (1) The preserved Phytophthora sojae strain is activated and grown on V8 medium, and the activated growth time is 5-6 days.
[0037] (2) Wash and dry the tomatoes and lettuce and grind them with a tissue grinder. Filter with four layers of gauze to remove residue. Mix tomato juice and lettuce juice in a ratio of 1:2.
[0038] (3) Add CaCO at a ratio of 0.02% (w / v) 3 , and then add deionized water to prepare 50% vegetable juice culture medium. The temperature is 120-122°C, and the sterilization time is 29-31min.
[0039] (4) Cultivate the activated Phytophthora sojae strain on the LBA medium at a temperature of 24-26°C under dark conditions for 3-4 days and then use a puncher (d=5mm) to take the bacteria plate along the edge of the colony .
[0040] (5) Put 4 plates of bacteria into 14-16ml culture medium, culture at 24-26°C under dark conditions, and the culture time is...
Embodiment 3
[0062] Determination of potted control effect
[0063] The difference between embodiment 3 and embodiment 2 is: to grow the root of soybean seedling with 2 true leaves, such as Bacillus sp. bacillus inoculum, and each strain is filled with 15 ± 1ml. The seedlings poured with an equal volume of NB medium at the root were used as the control. After 3-4 days, the zoospore suspension of Phytophthora sojae was poured into the roots of soybean seedlings, and 30±1ml was poured into each plant. Each treatment was repeated 5 times. Cultivate at 24-26°C, and calculate the incidence rate, disease index and control effect after 9-11 days. The calculation formula of each parameter is as follows:
[0064] Incidence rate (%) = (number of diseased plants / total number of plants) × 100%
[0065] Disease index = 100 × ∑ (number of plants of all levels of severity × representative value of each level) / (number of investigated plants × representative value of the highest level)
[0066] Prev...
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