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Detection method for contents of six volatile components in vegetable drugs of Longhurendan

A technology for volatile components and detection methods, applied in the field of content determination of traditional Chinese medicine components, can solve the problems of long detection time and complex processing steps.

Inactive Publication Date: 2015-09-30
SHANGHAI ZHONGHUA PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the quality control methods of Longhu Rendan mainly include microscopic identification, thin layer identification, high performance liquid chromatography and gas chromatography. Zhang Yujie et al. used gas chromatography to determine the content of borneol and menthol in it. A single component in Longhu Rendan, the detection time is long, the pre-treatment steps of the sample detection are complicated, etc.
So far, there is no research report on the simultaneous detection of multiple components in Longhurendan, and there is no (1) menthol, (2) isoborneol, (3) borneol, (4) anethole, ( 5) Simultaneous determination method of 6 kinds of volatile components of eugenol and (6) acetyl eugenol

Method used

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  • Detection method for contents of six volatile components in vegetable drugs of Longhurendan
  • Detection method for contents of six volatile components in vegetable drugs of Longhurendan
  • Detection method for contents of six volatile components in vegetable drugs of Longhurendan

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] 1. Instruments and reagents

[0047] Instrument: Agilent7890A gas phase-Agilent7000B triple quadrupole mass spectrometer (Agilent Technologies, Inc., USA)

[0048] Test medicine: Longhu Rendan botanical medicine

[0049] Other reagents: ethyl acetate, butyl acetate, isopropanol, chloroform.

[0050] 2. Method

[0051] (1) Prepare the test solution: take Longhu Rendan herbal medicinal solvent to prepare a 0.5-5mg / ml test solution;

[0052] Preparation of the reference substance solution: Accurately weigh an appropriate amount of the reference substance, place it in a 10mL volumetric flask, dissolve it with a solvent and dilute to the mark. Shake well to obtain the stock solution of each reference substance, and store in a refrigerator at 4°C.

[0053] Precisely measure the appropriate amount of each stock solution and dilute with ethyl acetate to obtain a mixed standard solution of each reference substance. Add 10-20 μL of internal standard naphthalene to the mixed ...

Embodiment 2

[0070] 1. Instruments and reagents

[0071] Instrument: Agilent7890A gas phase-Agilent7000B triple quadrupole mass spectrometer (Agilent Technologies, Inc., USA)

[0072] Test medicine: Longhu Rendan botanical medicine

[0073] Other reagents: ethyl acetate, butyl acetate, isopropanol, chloroform.

[0074] 2. Method

[0075] (1) Prepare the test solution: take Longhu Rendan herbal medicinal solvent to prepare a 0.5-5mg / ml test solution;

[0076] Preparation of the reference substance solution: Accurately weigh an appropriate amount of the reference substance, place it in a 10mL volumetric flask, dissolve it with a solvent and dilute to the mark. Shake well to obtain the stock solution of each reference substance, and store in a refrigerator at 4°C.

[0077] Precisely measure the appropriate amount of each stock solution and dilute with isopropanol to obtain a mixed standard solution of each reference substance. Add 10-20 μL of internal standard naphthalene to the mixed re...

Embodiment 3

[0094] 1. Instruments and reagents

[0095] Instrument: Agilent7890A gas phase-Agilent7000B triple quadrupole mass spectrometer (Agilent Technologies, Inc., USA)

[0096] Test medicine: Longhu Rendan botanical medicine

[0097] Other reagents: ethyl acetate, butyl acetate, isopropanol, chloroform.

[0098] 2. Method

[0099] (1) Prepare the test solution: take Longhu Rendan herbal medicinal solvent to prepare a 0.5-5mg / ml test solution;

[0100] Preparation of the reference substance solution: Accurately weigh an appropriate amount of the reference substance, place it in a 10mL volumetric flask, dissolve it with a solvent and dilute to the mark. Shake well to obtain the stock solution of each reference substance, and store in a refrigerator at 4°C.

[0101] Precisely measure the appropriate amount of each stock solution and dilute with ethyl acetate to obtain a mixed standard solution of each reference substance. Add 10-20 μL of internal standard naphthalene to the mixed ...

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Abstract

The invention provides a method for determining the contents of six volatile components in vegetable drugs of Longhurendan by using a gas chromatography-triple quadrupole mass spectrometry technology. The method comprises the following steps: firstly, preparing a test sample solution and a comparison product solution; detecting according to the following method respectively: feeding a sample into a VF-WAXms capillary tube chromatographic column according to the sample feeding amount of 1-10 microliters, wherein carrier gas is helium gas; adopting a program temperature rising method: maintaining an initial temperature of 60-90 DEG C for 1-2 minutes; raising the temprature to 240-260 DEG C by the temperature rising speed of 25-50 DEG C of each minute and maintaining for 1 minute, wherein the temperature of a sample feeding hole is 240-260 DEG C, the flow is not split, the flow speed is 2.5mL / min, the solvent delaying is 4 minutes and 2 microliters of the samples are fed, thereby obtaining ion chromatograms of test sample drugs and comparison product drugs; and determining all components and contents of all the components through spectrum contrast.

Description

technical field [0001] The invention relates to a content determination method and a quality control method of traditional Chinese medicine components, in particular to a content detection method and a quality control method of Longhu Rendan botanical medicine. Background technique [0002] Longhu Ren Dan (Z20025168) was created in 1909 by Mr. Huang Chujiu according to the ancient prescription "Zhuge Xingjun Powder". Its functions and indications are clearing away summer-heat and resuscitating, warding off filth and turbidity, dispelling wind and invigorating the stomach. In 1923, the "Dragon Tiger Brand" and "Rendan and Pattern" trademarks were applied to and approved by the government, and became the first standardized pharmaceutical registered trademarks in China, marking the beginning of the national pharmaceutical industry in Shanghai and even in China. Over the past century, the sales of Longhu Rendan products have lasted for a long time, and it has become an exclusiv...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 金家骅丁礼琴杜子荣马越鸣王天明
Owner SHANGHAI ZHONGHUA PHARMA
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