Degradation strain of herbicide 2, 4-D, produced inoculum and application thereof

A herbicide, 4-D technology, applied in the field of microorganisms, can solve the problems of crop damage economy, impact on food safety, strong adsorption and other problems, so as to solve the phytotoxicity of next crops and relieve the phytotoxicity of subsequent crops , the effect of strong resistance

Active Publication Date: 2015-10-07
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

2,4‐D degrades slowly and has strong adsorption, so it is easy to remain
In addition, the phytotoxicity

Method used

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  • Degradation strain of herbicide 2, 4-D, produced inoculum and application thereof
  • Degradation strain of herbicide 2, 4-D, produced inoculum and application thereof
  • Degradation strain of herbicide 2, 4-D, produced inoculum and application thereof

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Example Embodiment

[0031] Example 1

[0032] The present invention provides a strain capable of efficiently degrading (mineralizing) 2,4-D and a bacterial agent produced therefrom. The strain used is a Gram-negative bacterium LZ35, which is isolated from the soil of the Jiangsu Kunshan Lvlilai Pesticide Factory . The specific isolation and screening method of the strain is: take 5.0 g of soil sample and add it to 100 ml of inorganic salt liquid medium with 0.1% yeast powder, add 2,4-D to a final concentration of 50 ppm, and carry out enrichment culture at 30°C. Shaker speed is 150rpm·min -1 , Inoculate 10% of the inoculum into fresh inorganic salt liquid medium every 7 days, and subculture 3 times continuously. Then, inoculate with 1% of the inoculum to add fresh 20ml inorganic salt liquid medium with 2,4-D final concentration of 50ppm, and cultivate for 5 days. The content of 2,4-D was detected by UV scanner and liquid chromatography simultaneously, and it was found that 2,4-D in the enriched l...

Example Embodiment

[0039] Example 2

[0040] The original seed of the 2,4-D herbicide-degrading strain LZ35 of the present invention is activated on a petri dish, the degradation performance is measured, and it is inoculated on the slope of the test tube for use. The test tube seeds were inoculated into a 1000ml shake flask containing 200ml broth medium (3g beef extract, 10g peptone, 5g NaCl, 1000ml water, pH 7.0-7.2), cultured with constant temperature shaking to log phase, ready to inoculate seeds tank. 500 liters of seed tank, 400 liters of feed, medium formula: glucose 0.8%, (NH 4 ) 2 SO 4 1%, K 2 HPO 4 0.2%, MgSO 4 0.05%, NaCl 0.01%, CaCO 3 0.3%, yeast extract 0.02%, pH value 7.2-7.5. After feeding, sterilize with high pressure and humid heat at 121℃. After cooling to 33℃, inoculate the above-mentioned cultured shake flask strain into a 500 liter seed tank at an inoculum of 10%, and cultivate to the logarithmic growth phase with a stirring speed of 220 rpm / Min, the sterile air intake is 1:0...

Example Embodiment

[0041] Example 3

[0042] The original seed of the 2,4-D herbicide-degrading strain LZ35 of the present invention is activated on a petri dish, and the degradation performance is measured, and it is inoculated on the slope of the test tube for use. The test tube seeds were inoculated in a 1000ml shake flask containing 500ml broth medium (3g beef extract, 10g peptone, 5g NaCl, 1000ml water, pH 7.0-7.2), and cultured with constant temperature shaking to logarithmic phase. The cells were collected by centrifugation and resuspended in sterile water. Then take fresh test soil, add 2,4-D aqueous solution, and mix well so that the concentration of 2,4-D in the soil is 50mg·kg respectively -1 And 100mg·kg -1 Dry soil. At the same time, the treatment group was inoculated with fresh LZ35 strain bacterial suspension in the soil, and the blank control group was added with sterile water and stirred evenly. Divide the mixed soil into disposable plastic cups and use clean water to adjust th...

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Abstract

The invention discloses a degradation strain of herbicide 2, 4-D (2, 4-dichlorophenoxy acetic acid), a produced inoculum and application thereof. The strain is preserved in China Center For Type Culture Collection on April 15, 2015, and the strain preservation number is CCTCC NO:M2015235. The strain is gram staining reaction negative strain LZ35, which is identified as Achromobacter sp. The 2, 4-D degradation strain LZ35 can be applied to degradation of the herbicide 2, 4-D. A degradation inoculum produced from the 2, 4-D degradation strain LZ35 can degrade 99% of 100mg/L 2, 4-D in a liquid culture medium within 2 days, and direct application of the inoculum can significantly reduce crop phytotoxicity, solve the exceeding herbicide residue problem in agricultural production and produce non-toxic and nuisanceless green agricultural products.

Description

technical field [0001] The invention belongs to the field of microbes, and provides a herbicide 2,4-D degrading bacterial strain as well as the bacterial agent for its production and application. technical background [0002] Organochlorine pesticides are one of the most important and effective pesticides used before the 1980s. Because of their low price, high efficiency and broad spectrum, they have been widely used in the world. Among them, phenoxyacetic acid herbicides are produced and used on a large scale because of their low cost and high speed. 2,4‐D (2,4‐dichlorophenoxyacetic acid; 2,4‐Dichlorophenyloxy acetic acid) is a representative substance for the synthesis of auxin, and it is also a commonly used herbicide, which plays an important role in agriculture and forestry. It plays a significant role, especially widely used in the preservation of fruits (such as citrus). 2,4‐D can be used as a plant growth regulator at low concentrations below 30ppm, and as a herbic...

Claims

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Application Information

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IPC IPC(8): C12N1/20A62D3/02B09C1/10C12R1/025A62D101/04
CPCA62D3/02A62D2101/04A62D2101/28B09C1/10C12N1/205C12R2001/025
Inventor 蒋建东张龙杜方岭周庆新王军华陈凯李顺鹏
Owner NANJING AGRICULTURAL UNIVERSITY
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