Bacillus amyloliquefaciens, method for preparing solid inoculant thereof and application of solid inoculant

A technology of amyloliquefaciens spores and solid-state bacterial agents, applied in the biological field, can solve the problems that the solid-state bacterial agents of Bacillus amyloliquefaciens have not been reported, and achieve significant application value, reasonable formula, and low energy consumption

Inactive Publication Date: 2015-10-07
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is no report on the solid bacterial agent of Bacillus amyloliquefaciens

Method used

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  • Bacillus amyloliquefaciens, method for preparing solid inoculant thereof and application of solid inoculant
  • Bacillus amyloliquefaciens, method for preparing solid inoculant thereof and application of solid inoculant
  • Bacillus amyloliquefaciens, method for preparing solid inoculant thereof and application of solid inoculant

Examples

Experimental program
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Effect test

Embodiment 1

[0036] Get Bacillus amyloliquefaciens L-S60 described in the present invention, prepare Bacillus amyloliquefaciens solid bacterial agent according to the following steps:

[0037] (1) Get Bacillus amyloliquefaciens, streak on LB solid plate medium, and activate at 30°C for 24h; take the robust and typical colony after activation, inoculate in 10ml LB liquid medium, and cultivate at 30°C for 24h; Inoculated in 300ml LB liquid medium, cultured at 30°C for 24 hours, and the effective number of viable bacteria was 3.0×10 8 cfu / ml seed solution;

[0038] (2) The seed liquid is uniformly mixed with a solid aqueous medium at 15ml / 100g, and the solid aqueous medium is prepared from the following steps: get peanut cake powder 53g, wheat bran 31.4g, cottonseed meal 15g, sodium carbonate 0.2g of glucose, 0.2g of glucose, 0.2g of peptone and distilled water accounting for 30% of the total weight of all solid materials. Bacteria for 30 minutes, that is;

[0039] The mixture of the seed ...

Embodiment 2

[0042] Get Bacillus amyloliquefaciens L-S60 described in the present invention, prepare Bacillus amyloliquefaciens solid bacterial agent according to the following steps:

[0043] (1) Get Bacillus amyloliquefaciens, streak on LB solid plate medium, and activate at 30°C for 24h; take the robust and typical colony after activation, inoculate in 10ml LB liquid medium, and cultivate at 30°C for 24h; Inoculated in 300ml LB liquid medium, cultured at 30°C for 24 hours, and the effective number of viable bacteria was 7.0×10 8 cfu / ml seed solution;

[0044] (2) The seed liquid is mixed uniformly with the solid aqueous medium at 20ml / 100g, and the solid aqueous medium is prepared from the following steps: get peanut cake powder 25g, wheat bran 55g, cottonseed meal 17g, sodium carbonate 1.0 g, glucose 1.0g, peptone 1.0g, and distilled water accounting for 35% of the total weight of all solid materials, after stirring evenly, put it into a culture bottle at 350g / L, and intermittently st...

Embodiment 3

[0048] Get Bacillus amyloliquefaciens L-S60 described in the present invention, prepare Bacillus amyloliquefaciens solid bacterial agent according to the following steps:

[0049] (1) Get Bacillus amyloliquefaciens, streak on LB solid plate medium, activate at 30°C for 24h; take the robust and typical colony after activation, inoculate in 10ml LB liquid medium, and cultivate at 35°C for 24h; Inoculated in 300ml LB liquid medium, cultured at 30°C for 24h, the effective number of viable bacteria was 1.0×10 9 cfu / ml seed solution;

[0050] (2) Mix the seed liquid at 30ml / 100g with a solid aqueous medium, and the solid aqueous medium is prepared from the following steps: get peanut cake powder 53g, wheat bran 35g, cottonseed meal 11.5g, sodium carbonate 0.5g of glucose, 0.5g of glucose, 0.5g of peptone, and distilled water accounting for 45% of the total weight of all solid materials. Bacteria for 45 minutes, that is;

[0051] The mixture of seed liquid and solid aqueous medium...

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Abstract

The invention relates to bacillus amyloliquefaciens, solid inoculant of the bacillus amyloliquefaciens, a method for preparing the solid inoculant and application of the solid inoculant. The bacillus amyloliquefaciens is kept under CGMCCNO.10045. The bacillus amyloliquefaciens, the solid inoculant, the method and the application have the advantages that a process for fermenting the solid inoculant of the bacillus amyloliquefaciens is simple and is low in environmental pollution and energy consumption, and accordingly the bacillus amyloliquefaciens, the solid inoculant, the method and the application conform to national industrial policies; the solid inoculant of the bacillus amyloliquefaciens has a high effective viable bacterium number and has important application value in the aspect of plant growth promoting and particularly in the aspect of economic plant cultivation.

Description

technical field [0001] The invention belongs to the field of biological technology, and relates to a preparation method and application of bacillus amyloliquefaciens and a solid microbial agent thereof. Background technique [0002] In recent years, problems such as drug residues and soil pollution caused by excessive abuse of chemical fertilizers and pesticides have become increasingly serious. In modern times, with the enhancement of people's awareness of environmental protection and the increasing emphasis on pollution-free agricultural products, the research and development of new technologies and new products continues to deepen, and the research on exploring efficient, safe and environmentally friendly microbial agents to replace or partially replace chemical fertilizers and pesticides is increasing. [0003] Bacillus is a very important kind of plant rhizosphere growth-promoting bacteria resources, which have various functions such as secreting IAA, improving rhizosph...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P21/00A01N25/12C12R1/07
CPCA01N25/12A01N63/00C12N1/20C12N1/205C12R2001/07A61K2300/00
Inventor 李平兰游成真尚庆茂秦宇轩张志刚于亚琼王卉
Owner CHINA AGRI UNIV
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