Novel platelet antibody kit using micro-column gel technique and preparing method thereof

Abstract

The invention relates to a novel platelet antibody kit using the micro-column gel technique and a preparing method thereof. The novel platelet antibody kit comprises a micro-column gel card, indicating erythrocytes and platelet antigens. The preparing method includes: preparing the micro-column gel card and preparing anti-IgG sensitization aldehydated erythrocytes. The novel platelet antibody kit has the advantages that sensitivity is high, specificity is good, quality is stable, the detection process is short, the preparing method is simple and easy, result are easy to judge, mass samples can be detected at the same time, automation is easy, and the platelet typing technique is made a routine clinical test item.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to the composition, preparation method and application of a novel microcolumn gel method platelet antibody detection kit, which is suitable for clinical syndromes produced by various platelet antibodies, such as purpura after blood transfusion and ineffective platelet transfusion Diagnosis, prevention and treatment of diseases and other diseases. Background technique [0002] In clinical blood transfusion, with the continuous promotion of component blood transfusion, blood therapy has attracted more and more people's attention and has become a research hotspot. How to quickly, safely and effectively transfuse blood is often related to the life of patients and will become a development trend in the future. Platelet transfusion can reduce severe bleeding caused by thrombocytopenia, and has become an effective supportive therapy for patients with hematological diseases and tumors with radiot...

AI Technical Summary

Problems solved by technology

[0003] Currently existing platelet blood type detection techniques include: platelet immunofluorescence test, enzyme-linked immunosorbent test; mixed passive hemagglutination technique, mixed cell adhesion test (solid-phase technique), specific monoclonal antibody to platelet antigen immobilization test, etc. , but the operating procedures of these tests are relatively complicated, it takes a long time, and some required equipment and instruments are expensive, so platelet blood type matching has not yet become a routine test item for clinical platelet transfusion

The methods of SEPSA and MACE are commonly used in clinical platelet antibody screening, and the sensitivity and specificity are only 30% and 40%. PAIg can be seen to increase in many non-immune diseases, and the diagnostic reliability of immune thrombocytopenia is increasingly challenged

However, in the patent of 00105438, it is mentioned that in the micro-column gel method, fresh blood is used for indicating red blood cells, and the shelf life of indicating red blood cells is short, which is not conducive to the long-term stability of the kit.