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Preparation method of zeaxanthine

A technology of zeaxanthin and corn gluten powder, which is applied in the direction of organic chemistry, can solve the problems of high operating pressure, high operating cost, and high equipment requirements, and achieve the effects of increasing added value, good stability, and high pigment content

Active Publication Date: 2015-10-14
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Compared with traditional extraction methods, supercritical fluid extraction technology has obvious advantages, but due to its high operating pressure and high requirements for equipment, resulting in high operating costs, the current large-scale implementation in industry is still limited. limits

Method used

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  • Preparation method of zeaxanthine
  • Preparation method of zeaxanthine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Get corn gluten powder 500g, add water, control feed liquid mass volume ratio to be 1:10 (volume unit is L, mass unit is kg), add Alcalase2.4L protease, the amount of enzyme added is 5% of the corn gluten powder quality, enzyme The hydrolysis temperature is 50°C, the pH value is 8.0, and the enzymatic hydrolysis time is 60 minutes. After the enzymatic hydrolysis, the enzyme treatment was extinguished in a 90°C water bath, the pH value was adjusted to 4.0, and centrifuged at 8000g for 30 minutes. The obtained crude extract precipitate was dissolved and extracted with absolute ethanol. The ratio of solid to liquid is 1:10 (L / kg), and the extraction time is 4h. After the extraction was completed, the extract was rotary-evaporated with a vacuum rotary evaporator. The operating temperature of the rotary evaporation was 60° C., and the rotary evaporation time was 25 minutes. Finally, 21.25 g of refined zeaxanthin was obtained. As a result of the determination, the extract h...

Embodiment 2

[0029] Get corn gluten powder 1000g, pack in the beaker of 10L. Add water, control the mass-volume ratio of feed liquid to be 1:10 (volume unit is L, mass unit is kg), add Alcalase2.4L protease, the amount of enzyme added is 5% of the corn gluten powder quality, enzymolysis temperature is 55 ℃, The pH value is 8.0, and the enzymatic hydrolysis time is 120min. After the enzymatic hydrolysis, the enzyme treatment was extinguished in a 90°C water bath, the pH value was adjusted to 4.0, and centrifuged at 8000g for 30 minutes. The obtained crude extract precipitate was dissolved and extracted with absolute ethanol. The ratio of solid to liquid is 1:15 (L / kg), and the extraction time is 4h. After the extraction was completed, the extract was rotated with a vacuum rotary evaporator. The operating temperature of the rotary evaporation was 60° C., and the rotary evaporation time was 25 minutes. Finally, 34.81 g of refined zeaxanthin was obtained.

Embodiment 3

[0031] Get corn gluten powder 1000g, pack in the beaker of 10L. Add water, control the mass-volume ratio of feed liquid to be 1:10 (volume unit is L, mass unit is kg), add Alcalase2.4L protease, the amount of enzyme added is 5% of the corn gluten powder quality, enzymolysis temperature is 55 ℃, The pH value is 8.0, and the enzymatic hydrolysis time is 120min. After the enzymatic hydrolysis, the enzyme treatment was extinguished in a 90°C water bath, the pH value was adjusted to 4.0, and centrifuged at 8000g for 30 minutes. The precipitated crude extract was dissolved and extracted with n-hexane. The ratio of solid to liquid is 1:15 (L / kg), and the extraction time is 4h. After the extraction was completed, the extract was rotated with a vacuum rotary evaporator. The operating temperature of the rotary evaporation was 40° C., and the distillation time was 25 minutes. Finally, 30.23 g of refined zeaxanthin was obtained. After the above extraction is completed, the extract is s...

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Abstract

The invention discloses a preparation method of zeaxanthine. According to the preparation method, corn gluten meal is dissolved in water, Alcalase2.4L protease is added, enzymolysis temperature is controlled to be 45 to 60 DEG C, enzymolysis pH is adjusted to be 8.0 to 9.0, and enzymolysis time is controlled to be 45 to 90min, wherein adding amount of Alcalase2.4L protease accounts for 1 to 5% of the mass of corn gluten meal; after enzymolysis, pH value of an obtained product is adjusted to be 3.0 to 4.0, the product is subjected to centrifugation, and a supernate is discarded so as to obtain a precipitated enzymolysis product; the enzymolysis product is subjected to extraction with an organic solvent so as to obtain an extract liquor containing zeaxanthine; and at last zeaxanthine is obtained via rotary evaporation. Continuous operation of the preparation method can be realized; and the preparation method is convenient for realization of industrialized mass production, is capable of increasing additional value of corn gluten meal, and possesses excellent economic benefits. The prepared zeaxanthine grease is high in pigment content and stability, and is green and natural.

Description

technical field [0001] The invention belongs to the technical field of agricultural product processing, and in particular relates to a preparation method of zeaxanthin. technical background [0002] Corn gluten meal (CGM) is a kind of by-product produced in the wet processing of corn starch, which contains not only protein, starch and fat, but also a large amount of zeaxanthin. At present, CGM is usually used in the feed industry, but due to its low bioavailability, deep color and strong odor, its utilization is very limited. With the rapid development of the food industry, the production of corn starch has increased significantly, but the resulting CGM has been backlogged in large quantities. Long-term storage not only wastes a lot of resources, but also causes environmental pollution. Therefore, finding an effective and high value-added utilization method for CGM has become an urgent problem to be solved. Zeaxanthin is an oil-soluble carotenoid, which has various physiol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C403/24
Inventor 杨晓泉王超跃王永辉王金梅
Owner SOUTH CHINA UNIV OF TECH
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