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Surface treatment method for disposable plastic petri dish

A surface activation treatment and petri dish technology, which is applied in the fields of medicine and biology, can solve the problems of non-hydrophilicity, bacteria, difficult cell adherence culture, and environmental protection.

Inactive Publication Date: 2015-10-14
SUZHOU UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The current disposable plastic petri dishes, such as polystyrene (PS), polyethylene (PE), polycarbonate (PC), etc., are very difficult for bacteria and cells to adhere to and culture because their surfaces are not hydrophilic.
Moreover, high-molecular plastic materials cannot be sterilized by high-temperature methods. Currently, highly toxic ethylene oxide is used for sterilization and disinfection, which is extremely unfriendly to the environment.

Method used

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  • Surface treatment method for disposable plastic petri dish

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Experimental program
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Effect test

Embodiment 1

[0010] Such as figure 1 As shown, the plastic petri dish 8 is placed between the cathode plate 1 and the anode plate 2. In this embodiment, four layers can be arranged to improve efficiency.

[0011] Turn on the vacuum pump 4, and when the vacuum degree reaches 5Pa, open and adjust the nitrogen valve 5 to keep the vacuum degree at 30Pa, and apply high frequency and high voltage (40KHz, 1KV) between the cathode plate 1 and the anode plate 2. Glow discharge occurs between the anode plates, nitrogen plasma is generated, and the surface of the plastic culture dish is activated. After 2 minutes, the high-frequency high-voltage power supply and the nitrogen valve 5 are turned off in sequence.

[0012] Turn on and adjust the H 2 o 2 Steam valve 6 to keep the vacuum at 20Pa.

[0013] Then open and adjust the acrylic acid vapor valve 7 to keep the vacuum at 40Pa.

[0014] Turn on the high-frequency high-voltage power supply again, and at this time, a glow discharge is generated bet...

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Abstract

The invention discloses a surface treatment method for a disposable plastic petri dish. The method comprises the following steps: putting a plastic petri dish into a plasma reaction cavity; starting a vacuum pump for vacuumizing; when the vacuum degree is 5Pa, introducing nitrogen, and adjusting a valve to keep the vacuum degree at 30Pa; applying high frequency of 40KHz and high voltage of 1,000V to the negative and positive electrodes in the reaction chamber to generate glow discharge; 2 minutes later, closing the high-frequency high-voltage power supply and the nitrogen valve; opening a hydrogen peroxide steam valve to keep the vacuum degree at 20Pa; opening an acrylic acid steam valve to keep the vacuum degree at 40Pa; connecting the high-frequency high-voltage power supply again to start glow discharge; and performing hydrophilic treatment on the petri dish using the generated acrylic acid plasma, and performing disinfection treatment on the petri dish using the hydrogen peroxide plasma.

Description

technical field [0001] The invention relates to the technical fields of biology and medicine, in particular to the surface hydrophilic and disinfection treatment of disposable plastic culture dishes. Background technique [0002] At present, disposable plastic petri dishes are widely used in many fields such as biology, medicine, and chemical industry. Due to their low price, plastic products have become the first choice of polymer materials for experimental analysis in biology, medicine and other industries. [0003] The current disposable plastic culture dishes, such as polystyrene (PS), polyethylene (PE), polycarbonate (PC), etc., are very difficult to be used for the adherent culture of bacteria and cells because their surfaces are not hydrophilic. Moreover, high-molecular plastic materials cannot be sterilized by high-temperature methods. Currently, highly toxic ethylene oxide is used for sterilization and disinfection, which is extremely unfriendly to the environment....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J7/18C08J7/12A61L2/20A61L2/14A61L101/22
Inventor 田永静
Owner SUZHOU UNIV OF SCI & TECH
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