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Cupriavidus sp. YNS-85 and its application in soil restoration

A technology of YNS-85 and copper-loving bacteria, applied in the field of environmental microorganisms, can solve the problems of non-degradation, excessive pesticide residues, and low bacterial resources.

Active Publication Date: 2015-10-14
INST OF SOIL SCI CHINESE ACAD OF SCI
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  • Summary
  • Abstract
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  • Claims
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Problems solved by technology

With the expansion of the planting scale of Chinese herbal medicines, Chinese herbal medicines are vulnerable to pests and diseases, especially in seasons when pests and diseases are serious, there is a serious phenomenon of pesticide abuse, resulting in excessive pesticide residues in the soil and plants
Pentachloronitrobenzene (PCNB) is an organochlorine protective fungicide, which has a good control effect on seedling and soil-borne diseases of various vegetables, but its chemical properties are stable and not easy to be degraded. With the continuous increase of the dosage, the environmental and health problems caused by it are also becoming more and more prominent.
To solve this problem, researchers at home and abroad have also screened out a number of PCNB-degrading bacteria, such as Streptomyces aureofaciens, S.cyanescens, M.racemosus, white rot fungus (Phanerochaete-chrysosporium), Pseudomonas aeruginosa, etc., but they are mainly fungal-type degrading bacteria, and the bacterial resources are less, and no germplasm resources of copper greedy bacteria that can degrade PCNB have been found.

Method used

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  • Cupriavidus sp. YNS-85 and its application in soil restoration
  • Cupriavidus sp. YNS-85 and its application in soil restoration
  • Cupriavidus sp. YNS-85 and its application in soil restoration

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Embodiment 1

[0017] 1. Materials and methods

[0018] 1.1 Materials and reagents

[0019] The soil tested for bacteria screening was pentachloronitrobenzene-contaminated soil in Wenshan, Yunnan. The standard PCNB was purchased from Beijing Bailingwei Chemical Technology Co., Ltd. Chromatographically pure n-hexane. Anhydrous sodium sulfate (analytical pure) was activated at 400°C for 6h, cooled and stored in a glass desiccator until use.

[0020] 1.2 Culture medium composition

[0021] LB liquid medium: peptone 10.00g, yeast powder 5.00g, sodium chloride 10.00g, add deionized water to 1L, adjust pH to 7.0, solid medium and then add agar at a mass ratio of 1.5%-2.0%.

[0022] Basal salt medium: KH 2 PO 4 1.00g, Na 2 HPO 4 1.2g, NH 4 NO 3 0.50g, MgSO 4 ·7H 2 O 0.20g, FeSO 4 ·7H 2 O 0.005g, trace element solution 10mL, add ultrapure water to 1L. With 2mol / L HNO 3 and 2mol / L NaOH solution to adjust the pH value of the medium to 7.0.

[0023] Trace element solution: Ca(OH) 2...

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Abstract

The invention relates to a Cupriavidus sp. YNS-85 and its application in soil restoration. The above strain is preserved in a preservation unit assigned by State Intellectual Property on April 24, 2015 with the preservation number of CGMCC No.10740, and the preservation unit is China General Microbiological Culture Collection Center. The strain can degrade pentachloronitrobenzene, and allows the pentachloronitrobenzene degradation rate after culture is carried out in a solution system for 7d to be 79.4% and the pentachloronitrobenzene degradation rate after culture is carried out in soil for 30d to be 56.9%.

Description

technical field [0001] The invention belongs to the technical field of environmental microbes, and in particular relates to the isolation and identification of pentachloronitrobenzene-degrading bacteria for a pesticide fungicide and its soil restoration effect. Background technique [0002] The research and application of Chinese herbal medicine has a history of thousands of years, and its unique therapeutic effect is favored by people. Due to my country's complex landform environment and climatic conditions, it has bred extremely rich resources of Chinese herbal medicines. At present, the total number of traditional Chinese medicine resources in my country has reached 12,807, including 11,146 kinds of plants, 1,581 kinds of animal medicines, and 80 kinds of mineral medicines. China is the largest producer of medicinal materials in the world. Most of the Chinese herbal medicines rely on artificial cultivation. The planting area of ​​medicinal materials in the country exceed...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B09C1/10C12R1/01
CPCB09C1/10C12N1/20C12N1/205C12R2001/01
Inventor 滕应朱烨马文亭李振高任文杰骆永明
Owner INST OF SOIL SCI CHINESE ACAD OF SCI
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