Gas-producing photoacoustic imaging contrast agent and preparation method thereof

A photoacoustic imaging and contrast agent technology, applied in the field of clinical medicine, can solve problems that cannot be ruled out, harmful to the body, and expensive, and achieve the effects of low cost, easy source, and improved diagnostic value

Inactive Publication Date: 2017-12-05
CHONGQING MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] One of the purposes of the present invention is to overcome the problem that the contrast agent in the prior art is harmful to the body, cannot be completely eliminated from the body, and is very expensive, and to provide a new type of gas-producing photoacoustic contrast agent that is safe, cheap, and has no side effects The preparation method thereof is used in photoacoustic imaging technology

Method used

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  • Gas-producing photoacoustic imaging contrast agent and preparation method thereof
  • Gas-producing photoacoustic imaging contrast agent and preparation method thereof
  • Gas-producing photoacoustic imaging contrast agent and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 Preparation of photoacoustic imaging contrast agent

[0037] 1. Selection of main materials: polyethylene glycol-distearoylphosphatidylethanolamine (DSPE-PEG), dipalmitoylphosphatidylcholine (DPPC), cholesterol, ammonium bicarbonate (NH 4 HCO 3 ).

[0038] 2. Preparation of photoacoustic imaging contrast agent: Ammonium bicarbonate lipid microspheres were prepared by thin film hydration method and extrusion method. Liposome microspheres were filled with ammonium bicarbonate saturated solution, outsourcing lipid, and then removed by dialysis Ammonium bicarbonate outside the liposomes to obtain liposome microspheres.

[0039] 3. Preparation steps:

[0040] Dissolve 10mgDPPC, 1mgDSPE-PEG, and 1mg cholesterol in 10ml chloroform. After fully dissolving to a clear and transparent degree, put it on a rotary evaporator and rotate it at a temperature of 45°C, for 50 minutes, and a rotation speed of 60r / min. The obtained phospholipid membrane was added with 5ml of a...

Embodiment 2

[0041] Example 2 Preparation of photoacoustic imaging contrast agent

[0042] 1. Selection of main materials: polyethylene glycol-distearoylphosphatidylethanolamine (DSPE-PEG), dipalmitoylphosphatidylcholine (DPPC), cholesterol, ammonium bicarbonate (NH 4 HCO 3 ).

[0043] 2. Preparation of photoacoustic imaging contrast agent: Prepare ammonium bicarbonate lipid microspheres (ammonium bicarbonate saturated solution inside, lipid outsourcing) by thin film hydration method and extrusion method, and then remove the liposomes by dialysis. ammonium bicarbonate to obtain liposome microspheres.

[0044] 3. Preparation steps:

[0045] Step 1: Dissolving phospholipids: Add 1mgDSPE-PEG, 10mgDPPC and 1mg cholesterol to 10ml chloroform, shake it by hand until fully dissolved, and form a clear mixed solution of phospholipids and chloroform;

[0046] Step 2: Prepare phospholipid film: place the mixed solution obtained in step 1 on a rotary evaporator to rotate at a speed of 40r / min, time:...

Embodiment 3

[0049] Example 3 Preparation of photoacoustic imaging contrast agent

[0050] 1. Selection of main materials: polyethylene glycol-distearoylphosphatidylethanolamine (DSPE-PEG), dipalmitoylphosphatidylcholine (DPPC), cholesterol, ammonium bicarbonate (NH 4 HCO 3 )

[0051] 2. Preparation of photoacoustic imaging contrast agent: Prepare ammonium bicarbonate lipid microspheres (ammonium bicarbonate saturated solution inside, lipid outsourcing) by thin film hydration method and extrusion method, and then remove the liposomes by dialysis. ammonium bicarbonate to obtain liposome microspheres.

[0052] 3. Preparation steps:

[0053] Step 1: Dissolving phospholipids: Add 1mgDSPE-PEG, 10mgDPPC and 1mg cholesterol to 10ml chloroform, shake it by hand until fully dissolved, and form a clear mixed solution of phospholipids and chloroform;

[0054] Step 2: Preparation of phospholipid film: Place the mixed solution obtained in step 1 on a rotary evaporator to rotate at a speed of 80 r / m...

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Abstract

The invention discloses a novel gas-producing photoacoustic imaging contrast agent and a preparation method thereof, belonging to the field of clinical medicine. A new type of gas-producing photoacoustic imaging contrast agent, the photoacoustic contrast agent includes a solution, microspheres and gas, the solution is a saturated solution of ammonium bicarbonate, wrapped in the microspheres; the microspheres are lipid Plastid microspheres, the formula of the liposome microsphere wall is: polyethylene glycol-distearoylphosphatidylethanolamine, dipalmitoylphosphatidylcholine, cholesterol; the present invention has the following advantages: adopt bicarbonate Ammonium saturated solution is not only safe, effective and reduces production costs; it can be used for photoacoustic imaging of superficial tissues; the produced microspheres are stored at low temperature; the membrane material is non-toxic and has no side effects; the microspheres have small particle size, stable performance and excellent imaging effect Good; does not affect systemic or systemic hemodynamics.

Description

technical field [0001] The invention relates to a gas-producing photoacoustic imaging contrast agent and a preparation method thereof, belonging to the field of clinical medicine. Background technique [0002] Photoacoustic imaging is an emerging imaging technology developed in the 21st century, which is mainly used in the fields of physics and chemistry. With the continuous development of science and technology, the research of photoacoustic imaging has also made remarkable progress, and it has begun to be applied in the field of biomedicine. Photoacoustic imaging is to use the photoacoustic effect to achieve the purpose of distinguishing the different tissue structures and functions of the test object through the difference in the light absorption rate of the organism, which leads to the difference in the sound wave. It combines the high penetrating power of ultrasound and the optical The high-contrast characteristics of the system, so it has higher contrast and resolutio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K49/22
Inventor 夏纪筑冯罡王志刚夏晓蓉郝兰
Owner CHONGQING MEDICAL UNIVERSITY
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