Efficiency-improving method of compound bio-based chemical fertilizer and efficiency-enhancing chemical fertilizer
A bio-based and chemical fertilizer technology, applied in the direction of fertilizer mixture, bio-organic part treatment, organic fertilizer, etc., can solve the problems of environmental risks, high price of chemical-based materials, etc., to improve crop quality, control loss, and increase crop biomass Effect
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[0044] In order to further explain the preparation method of the chemical fertilizer efficiency enhancement factor, the present invention further provides a more detailed example.
[0045] A loop of bacterial cells was scraped from the preservation slant of CSF-1 strain and inoculated into a 300-ml Erlenmeyer flask containing 20 ml of seed medium. Incubate for 14 hours at 37°C with a shaker speed of 200 rpm. Then inoculate it into a 1000ml Erlenmeyer flask containing 150ml fermentation medium at 1:100 and cultivate for 12-16 hours to prepare a bacterial suspension (solid-state fermentation seed liquid) for later use.
[0046] Composition of seed medium and fermentation medium (calculated in 1000mL medium): maltose 40g, soluble starch 40g, glutamic acid 20g, MgSO 4 0.5g, KCl 0.5g, KH 2 PO 4 1g, FeSO 4 ·7H 2 O0.15g, MnSO 4 0.005g, CuSO 4 ·5H 2 O 0.00016g, yeast extract 1g, pH 7.0-7.2. above medium in 1.0 x 10 5 Pa, sterilized at 121°C for 20min.
[0047] The compos...
Embodiment 1
[0054] The surface of the downstream substrate after solid fermentation has a white or light brown film, and thick mucus can be seen with needles and filaments can be pulled out, indicating that a γ-polyglutamic acid-like polymer is synthesized by fermentation. The yield was detected to be 40-60 g / kg (material), and the molecular weight was between 40-150 kDa.
[0055] Dry the fermentation substrate, weigh 0.1 g and extract it with 4 ml of pure water (2.5% concentration). After the insoluble particles are precipitated, take the supernatant (extract solution), and the concentration of the supernatant is 0.87%. The flocculation effect of the supernatant on soil colloids was tested.
[0056] A 2.5% polyaluminum chloride solution was prepared as a control to compare the flocculation effect of the two on soil colloids.
[0057] The soil was dried and pulverized, and 0.4 g was weighed into 40 ml of pure water to prepare a suspension. A certain amount of leaching solution was added...
Embodiment 2
[0063] The weight ratio of soybean meal-corn germ meal-attapulgite in the solid fermentation medium used to prepare 1kg of compound bio-based urea for improving efficacy is 60-20-20.
[0064] Take 6 acrylic tubes with a length of 490 mm and an inner diameter of 14 mm. Tubes 1, 2, and 3 are set as the control group, and tubes 4, 5, and 6 are set as the experimental group. The six tubes were first filled with 45g of 80-100 mesh quartz sand (about 200mm in height), the control group was filled with a mixture of 0.1g urea and 1g quartz sand, and finally covered with 10g quartz sand; while the experimental group was filled with 0.1g urea, 0.01g efficiency factor Mix with 1g of quartz sand, and finally cover with 10g of quartz sand.
[0065] The 6 pipes are placed vertically on the support, and the water is leached from above, and the water inlet rate is 0.1ml / min. The filtrate was collected every 30 minutes, and the filtrate was collected in three time periods. The quality of the ...
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