Preparation method for laundry detergent based on fish intestine proteinase
A technology for laundry detergent and protease, which is applied in chemical instruments and methods, detergent compounding agents, detergent compositions, etc., can solve the problems of unfavorable water treatment and environmental protection, limited detergency of laundry detergent, and high production cost, and achieves good Decontamination effect, broad market and prospects, effect of sufficient raw materials
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[0025] A preparation method based on fish intestinal protease laundry detergent, comprising the following steps:
[0026] Step 1. Extraction of proteolytic enzyme solution: select suitable fish (one or more of small yellow croaker, large yellow croaker, mullet, squid, mackerel, cod, carp, grass carp, rice field eel, etc.), and cut out the fish intestines; Then the fish intestines are shredded and cleaned with deionized water to obtain the cleaned fish intestines;
[0027] Then add deionized water to the cleaned fish intestines and carry out homogenization treatment in a high-speed homogenizer to obtain a homogenate liquid of fish intestines. The weight ratio of fish intestines and deionized water is 1: (2-4) (exemplarily, It can be any value in 1:2, 1:2.5, 1:3, 1:3.5, 1:4, etc. or any range between the two), and the homogenate temperature is 0-4°C (exemplarily, it can be 0°C, 1°C, 2°C, 3°C, 4°C, etc. or any range between them), the time is 20-70s (for example, it can be 20s, ...
Embodiment 1
[0043] The preparation method of the fish intestinal protease laundry liquid of the present embodiment comprises the steps:
[0044] (1) Pretreatment of fish intestines: take small yellow croaker intestines, cut them into pieces with scissors, wash blood and foreign matter with distilled water, obtain the cleaned fish intestines, and weigh them.
[0045] (2) Homogenization: Take 60 g of washed fish intestines and mix them with 120 mL of deionized water, add them to a high-speed homogenizer for homogenization for 30 seconds, and obtain a fish intestine homogenate.
[0046] (3) Extraction: centrifuge the fish intestine homogenate at 4°C and 12000r / min for 30min, and take the supernatant; measure 60ml of the supernatant, add 120ml of n-butanol (4°C), and place it at 4°C Extract for 5 hours to obtain fish intestine extract.
[0047] (4) Centrifugation: The fish intestine extract was refrigerated and centrifuged at 4°C and 12000 r / min for 30 minutes, and the supernatant was taken ...
Embodiment 2
[0051] The preparation method of the fish intestinal protease laundry liquid of the present embodiment comprises the steps:
[0052] (1) Pretreatment of fish intestines: take small yellow croaker intestines, cut them into pieces with scissors, wash blood and foreign matter with distilled water, obtain the cleaned fish intestines, and weigh them.
[0053] (2) Homogenization: take 60 g of washed fish intestines and mix them with 120 mL of deionized water, add them to a high-speed homogenizer for homogenization for 40 seconds, and obtain a fish intestine homogenate.
[0054] (3) Extraction: centrifuge the fish intestine homogenate at 4°C and 10000r / min for 30min, and take the supernatant; measure 60ml of the supernatant, add 120ml of n-butanol (4°C), and place it at 4°C Extract for 5 hours to obtain fish intestine extract.
[0055] (4) Centrifugation: The fish intestine extract was refrigerated and centrifuged at 4°C and 12000 r / min for 30 minutes, and the supernatant was taken ...
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