Decellularized anti-calcification heart patch and preparation method thereof

A decellularized, cardiac technique applied in the field of biomaterials in tissue engineering

Active Publication Date: 2015-10-28
SHAANXI BOYU REGENERATIVE MEDICINE CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, both similar heart patch products and pericardial materials treated by doctors before surgery are treated with glutaraldehyde to reduce the immunogenicity of raw materials, but glutaraldehyde has high toxicity, and the pericardium treated with glutaraldehyde During the degradation process of the material in vivo, glutaraldehyde molecules will be released when the collagen cross-linked by glutaraldehyde in the material, which affects the safety of the operation; at the same time, the glutaraldehyde-treated pericardial material has Changes in the collagen structure can easily lead to the formation of scar tissue after implantation, and the degradation time of the material is prolonged, making it easier to calcify

Method used

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  • Decellularized anti-calcification heart patch and preparation method thereof
  • Decellularized anti-calcification heart patch and preparation method thereof
  • Decellularized anti-calcification heart patch and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1: Preparation of decellularized anti-calcification bovine pericardium patch

[0033] Step 1. Pretreatment of the pericardium material: clean the blood and adipose tissue on the surface of the fresh bovine pericardium material, add isopropanol at a mass ratio of 1:10, shake at 120 rpm for 12 hours, and wash with purified water.

[0034] Step 2, decellularized anti-calcification treatment: place the bovine pericardium obtained in step 1 in a 3M sodium chloride mixed solution of 0.5% SDS and 0.75% sodium hydroxide, shake at 120 rpm for 10 minutes at 37°C, and then Purified water was shaken at 120rpm for 90 minutes, which was regarded as a cycle, and a total of 3 cycles were processed, and finally washed with purified water until neutral.

[0035] Step 3, terminal sterilization: use 15KGy dose of Co 60 The bovine pericardium obtained in step 2 was irradiated.

[0036] The bovine pericardium material has a relatively dense structure. In this example, a mixed solut...

Embodiment 2

[0037] Example 2: Preparation of acellular anti-calcification porcine pericardial heart patch

[0038] Step 1. Pretreatment of the pericardial material: clean the fresh porcine pericardial material from blood stains and adipose tissue on its surface, add acetone at a mass ratio of 1:15, vibrate at 120 rpm for 12 hours, and wash with purified water.

[0039] Step 2, decellularized anti-calcification treatment: place the porcine pericardium obtained in step 1 in a 1.5M sodium chloride mixed solution of 0.3% SDS and 0.5% sodium hydroxide, and shake at 100 rpm for 10 minutes at 37°C, and then Vibrated with purified water at 100rpm for 60 minutes, as a cycle, a total of 2 cycles, and finally washed with purified water until neutral.

[0040] Step 3, terminal sterilization: use 100keV electron beam to process the porcine pericardium obtained in step 2.

[0041] Porcine pericardium is looser in structure than bovine pericardium, and has higher fat content. Therefore, in step 1, a ...

Embodiment 3

[0043] Step 1. Pretreatment of the pericardial material: clean the blood and adipose tissue on the surface of the fresh bovine pericardial material, add acetone at a mass ratio of 1:5, shake at 120 rpm for 16 hours, and wash with purified water.

[0044] Step 2, decellularized anti-calcification treatment: place the bovine pericardium obtained in step 1 in a mixed solution of 0.2% SDS and 0.4% potassium hydroxide in 2.5M sodium chloride, and shake at 130 rpm for 20 minutes at 37°C, and then Vibrated with purified water at 130rpm for 90 minutes, as a cycle, a total of 2 cycles, and finally washed with purified water until neutral.

[0045] Step 3, terminal sterilization: the bovine pericardium material obtained in step 2 was sterilized with ethylene oxide for 8 hours.

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Abstract

The invention relates to a preparation method of a decellularized anti-calcification heart patch. The method mainly comprises the steps that raw material pericardial tissue is degreased through an organic reagent and processed by a mixed solution of a high salt, a surface active agent and alkali, and finally sterilization treatment is performed to obtain the decellularized anti-calcification heart patch. According to the obtained heart patch, due to the special decellularized anti-calcification technology, the collagenous fiber three-dimensional pore structure of the raw materials is reserved, meanwhile, cells contained in the materials are effectively removed, and the immunogenicity is lowered; the materials can guide cells to grow in the materials, scar tissue generation is reduced, and the good anti-calcification ability is achieved; the excellent mechanical property is achieved, arterio-venous pressure difference between heart chambers can be resisted, and the repair effect is ensured. The preparation method is applicable to atrial septal defects, ventricular septal defects, aortic stenosis and the like caused by the congenital heart disease.

Description

technical field [0001] The invention belongs to the biological material technology of tissue engineering, and specifically relates to a decellularized anti-calcification heart patch and a preparation method thereof. Background technique [0002] According to statistics, there are more than 2 million congenital heart disease patients in my country, and about 160,000 children with congenital heart disease are born every year, and 80% of the children need to use patches for heart repair surgery. [0003] Since the heart defects caused by congenital heart disease are mainly symptoms of atrial septal defect, ventricular septal defect and patent ductus arteriosus, heart repair materials have certain performance requirements: 1) Good mechanical properties to resist damage caused by atrium or ventricle 2) Degradability, because the heart is rich in blood, the fiber wrapping will be completed about 1 week after the patch is placed at the repair site. There is a greater possibility ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36
Inventor 耿阳雪
Owner SHAANXI BOYU REGENERATIVE MEDICINE CO LTD
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