The invention relates to a testing device for a relationship between a pre-tightening force and a torque for threaded fastening connection. The testing device comprises a torque sensor, a torque sensor supporting seat, a three-jaw chuck, a right article placement table, a right clamp, a left clamp, a long screw, a limiting block, a left article placement table, a clamp seat, a supporting seat, a smooth guide rail, a double-end screw, a hand wheel, a pressing plate, a pressure sensor, a chain, a chain wheel, a nut and a fixed pressing plate bolt. By adoption of the testing device, assembly connection of threaded fasteners which comprise bolts with different lengths and connected pieces with different thicknesses can be effectively stimulated. The testing device is slightly influenced by a bolt structure and convenient in test operation, has a function of synchronously measuring an installation torque and the pre-tightening force, and provides test conditions for acquisition of the relationship (a tension relationship) between the torque and the pre-tightening force.