Carboxymethylated ganoderma lucidum polysaccharide, synthesis and purification method and application thereof as tobacco humectant

A technology of carboxymethylated Ganoderma lucidum and a purification method, which is applied in the field of humectants to achieve obvious moisturizing effects, broad promotion and application prospects, and significant industrial application value

Inactive Publication Date: 2015-10-28
CHINA TOBACCO HENAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no relevant reports on the application of carboxymethylated Ganoderma lucidum polysaccharides as tobacco humectants in cigarettes at home and abroad

Method used

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  • Carboxymethylated ganoderma lucidum polysaccharide, synthesis and purification method and application thereof as tobacco humectant
  • Carboxymethylated ganoderma lucidum polysaccharide, synthesis and purification method and application thereof as tobacco humectant

Examples

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Embodiment 1

[0022] A method for synthesizing and purifying carboxymethylated ganoderma lucidum polysaccharides, comprising the following steps: adding 14g NaOH and 120mL solvent (ionic liquid / water=1.2:1, mass ratio) to a three-necked flask, stirring at room temperature until the solution is clear, adding 10g The Ganoderma lucidum polysaccharide was vigorously stirred for 20 minutes until fully dissolved, then slowly added 16.8g of chloroacetic acid, stirred and reacted at 65°C for 3 hours, cooled to room temperature, adjusted to pH 7 with dilute acetic acid, precipitated with alcohol, filtered, and collected the precipitate.

[0023] The precipitate was dissolved with an appropriate amount of water, precipitated with 3 times the volume of 95% ethanol, and centrifuged at 4000r / m for 20min. The precipitate was collected again and dialyzed against running water using a dialysis bag. First dialyze with tap water for 24 hours, then dialyze with distilled water for 24 hours, and finally freeze...

Embodiment 2

[0026] A method for synthesizing and purifying carboxymethylated ganoderma lucidum polysaccharides, comprising the following steps: adding 16g NaOH and 120mL solvent (ionic liquid / water=1.2:1, mass ratio) to a three-necked flask, stirring at room temperature until the solution is clear, adding 10g Ganoderma lucidum polysaccharide was vigorously stirred for 30 minutes until fully dissolved, then slowly added 16.8g of chloroacetic acid, stirred and reacted at 75°C for 3 hours, cooled to room temperature, adjusted to pH 7 with dilute acetic acid, precipitated with alcohol, filtered, and collected the precipitate.

[0027] The precipitate was dissolved with an appropriate amount of water, precipitated with 3 times the volume of 95% ethanol, and centrifuged at 4000r / m for 20min. The precipitate was collected again and dialyzed against running water using a dialysis bag. First dialyze with tap water for 24 hours, then dialyze with distilled water for 24 hours, and finally freeze-dry...

Embodiment 3

[0030] A method for synthesizing and purifying carboxymethylated ganoderma lucidum polysaccharides, comprising the following steps: adding 18gNaOH and 120mL solvent (ionic liquid / water=1.2:1, mass ratio) into a three-necked flask, stirring at room temperature until the solution is clear, adding 10g Ganoderma lucidum polysaccharide was vigorously stirred for 20min until fully dissolved, then slowly added 16.8g chloroacetic acid, stirred and reacted at 85°C for 4h, cooled to room temperature, adjusted to pH 7 with dilute acetic acid, precipitated with alcohol, filtered, and collected the precipitate.

[0031] The precipitate was dissolved with an appropriate amount of water, precipitated with 3 times the volume of 95% ethanol, and centrifuged at 4000r / m for 20min. The precipitate was collected again and dialyzed against running water using a dialysis bag. First dialyze with tap water for 24 hours, then dialyze with distilled water for 24 hours, and finally freeze-dry to obtain l...

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Abstract

The invention relates to a synthesis and purification method of carboxymethylated ganoderma lucidum polysaccharide. The method includes the steps of: adding ganoderma lucidum polysaccharide and sodium hydroxide into a solvent to conduct stirring dissolving, then adding chloroacetic acid, carrying out stirring reaction at 65-85DEG C for 2-4h, performing cooling, adjusting the pH value to neutral, conducting alcohol precipitation and filtering, collecting the precipitate, then dissolving the precipitate in water, and carrying out alcohol precipitation, dialysis, and freeze drying to obtain light brown cotton-like carboxymethylated ganoderma lucidum crude polysaccharide. Test proves that: the carboxymethylated ganoderma lucidum polysaccharide provided by the invention has very good physical and sensory moisturizing effects, also can reduce cigarette stimulation and miscellaneous gas, improves the mellow and full sense and comfort of cigarette smoke, and can applied as an excellent performance tobacco humectants in cigarettes.

Description

technical field [0001] The invention belongs to the technical field of humectants, and in particular relates to a carboxymethylated ganoderma polysaccharide, a synthesis and purification method and its application as a tobacco humectant. Background technique [0002] At present, polyhydroxy substances such as propylene glycol and sorbitol are mainly used as humectants in my country’s cigarette industry. The main purpose of using such humectants is to maintain the moisture content of shredded tobacco during processing and improve the processing resistance of shredded tobacco. Emollients are not ideal for maintaining the moisture content of finished cigarettes and improving smoking comfort. [0003] Polysaccharides are polymerized from a large number of monosaccharides, and a large number of hydroxyl groups form hydrogen bonds with water molecules, which have strong water holding capacity. Carboxymethylation of polysaccharides can not only improve their solubility, but also Fu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A24B15/40
Inventor 陈芝飞芦昶彤孙志涛郝辉宋金勇
Owner CHINA TOBACCO HENAN IND
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