Fixing method and use of tissue slice

Abstract

The invention provides a fixing method and a use of a tissue slice; a quick-drying glue is rapidly dropped on a glass slide and evenly smeared into a thin layer, a tissue slice rapidly adheres onto the glass slide kiss-coated hung with the quick-drying glue, the tissue slice is closely bonded with the glass slide, and a slice falling-off phenomenon almost cannot happen; the operation is simple, the used glass slide is cheaper than a slice with a glue on markets, and the tissue slice can be used for HE staining, immunohistochemistry and various staining.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for adhering pathological slices and preventing slices from falling off. Background technique [0002] Detachment of tissue sections is always an inevitable problem during pathological staining. At present, the existing methods for preventing slices from falling mainly include collodion coating, polylysine and chrome vanadium gelatin treatment of slides, etc. Although hard tissue slices can also prevent slices from falling off, the slices are too thick (about 40-100 μm), the overlap of tissues is difficult to observe, and pathological staining cannot be performed. Paraffin and frozen sections can reach less than 10 μm, which can better observe the cell morphology, but there are always problems that some tissues are not firmly attached and are easy to fall off during staining, especially in the section staining of tissue engineering materials such as silk and art...

AI Technical Summary

Problems solved by technology

Although hard tissue slices can also prevent slices from falling off, the slices are too thick (about 40-100 μm), the overlap of tissues is difficult to observe, and pathological staining cannot be performed

Paraffin and frozen sections can reach less than 10 μm, which can better observe the cell morphology, but there are always problems that some tissues are not firmly attached and are easy to fall off during staining, especially in the section staining of tissue engineering materials such as silk and artificial ligaments , the drop phenomenon is more prominent

At the same time, in HE, ponceau trichrome and immunohistochemical staining, the phenomenon of tissue loss is particularly serious due to cumbersome staining steps and too long tissue immersion time.

Images