Application of arabidopsis AtACS2 gene in cotton drought resisting and early maturing
A technology of Arabidopsis thaliana and cotton, applied in the field of genetic engineering
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[0036] Example 1
[0037] Model plant Arabidopsis ( Arabidopsis thaliana L.) Provide good genetic material for revealing various physiological response mechanisms of plants. The study on the expression and regulation of related genes in Arabidopsis thaliana and the mechanism of plant response to water shortage and drought, water absorption and utilization can provide good clues and reference for the research of other large genome crops. Therefore, this example introduces the model plant simulation Research status of AtACS2 gene in Arabidopsis.
[0038] Arabidopsis AtACS2 (the number in the Arabidopsis genome is At1g01480) encodes an ACC synthase isoenzyme in Arabidopsis thaliana. The CDS is 1491 bp in length and encodes a protein of 496 amino acids.
[0039] Experimental Materials: The Arabidopsis AtACS2 loss-of-function mutant is a T-DNA insertion mutant, purchased from the Arabidopsis Resource Center (ABRC, Ohio State University).
[0040] Purpose: The drought resistance of Ara...
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[0051] Example 2
[0052] The GhACS2 gene of upland cotton is coded as GenBank: ABA00145.1 or DQ174260.1; the CDS length of this gene is 2296 bp, which encodes a protein of 663 amino acids, and the encoded protein belongs to the ACC synthase isoenzyme.
[0053] Upland cotton GhACS2 and Arabidopsis AtACS2 are homologous genes. Studies have shown that the sequence homology of the encoded protein is 63.17% (Data from the National Center for Biotechnology Information in the United States, such as figure 1 Shown), one of the highest values in the known ACS protein sequence comparison. Therefore, based on the functional study of the Arabidopsis AtACS2 gene in Example 1, the inventor believes that by enhancing the expression of the Arabidopsis AtACS2 gene and transfecting it into cotton, it is possible to give cotton drought tolerance, early maturity of cotton fiber without premature aging and other agronomic traits. On this basis, the inventor further conducted verification tests, wh...
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