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Method for simply and quickly screening antifungal drugs

An antifungal drug, rapid technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganism determination/inspection, etc. question

Active Publication Date: 2015-11-04
HANGZHOU ENJOY ENVIRONMENTAL PROTECTION TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, aquaculture units or individuals are still unable to distinguish the quality of drugs, and most of them are recommended by fishery drug dealers or purchased according to drug instructions. Huge economic losses for farmers

Method used

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  • Method for simply and quickly screening antifungal drugs
  • Method for simply and quickly screening antifungal drugs
  • Method for simply and quickly screening antifungal drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] Embodiment 1: XH medicine inhibits saprolegniasis test process

[0071] HGSM01 Saprolegniasis Strain: It was isolated in April 2013 from golden crucian carp oviparous water from Chuzhou Aquatic Breeding Farm in Anhui. In October 2013, the biological samples were sent to Shanghai Ocean University (National Aquatic Animal and Plant Pathogen Bank) for molecular biological identification of the strains and confirmed as Saprolegniasis.

[0072] XH: It is a raw material drug with a purity of 99%.

[0073] ⑷. Quickly determine the XH drug concentration range (the highest invalid concentration and the lowest effective concentration)

[0074] Prepare the test drug XH with 50PPM (mg / l) in distilled water; dilute it to 50, 25, 12.56., 6.25, ... 0ppm (mg / l) in a 12-well plate, and use distilled water as a control. In each concentration, put 2 silk pieces (2*2-5*5mm2) from the newly cultivated Saprolegnia colony after purification, cover with a cover to prevent moisture evaporatio...

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PUM

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Abstract

The invention discloses a method for simply and quickly screening antifungal drugs. The method comprises the following steps of preparation of a culture medium, separation of saprolegnia, purification culture, medicated bath drug application and the like. As long as the RI / RO ratio of a bacterial colony of an experimental group to a bacterial colony of a matched group is calculated, a mould suppression rate under the condition of the drug concentration can be quickly known according to a 'bacterial colony RI / RO ratio-mould suppression rate corresponding table / curve'. The method has the advantages of simplicity, easiness in implementation, rapidness, visual and the like, and is suitable for scientific research institutions, schools, technique popularization station and aquaculture companies.

Description

technical field [0001] The invention belongs to the technical field of aquaculture disease prevention and control, and in particular relates to a method for screening drugs for saprolegniasis of aquatic animals. The invention is simple and easy to implement, and is suitable for application in scientific research institutes, schools, technology extension stations and aquaculture companies. technical background [0002] Saprolegniasis is a kind of saprolegniasis parasitic disease on the surface of aquatic animals (such as: fish, shrimp, crab, turtle, frog, etc.). can be infected. When the surface skin of aquatic animals is damaged due to physical and chemical factors, or bacterial or viral infection, the zoospores of saprolegniasis invade the wound, absorb the nutrients in the skin and its tissues and grow rapidly, and the hyphae entwine and stick to the tissue cells of the wound. Attachment, tissue inflammation, necrosis, aquatic animals due to heavy burden, swimming disord...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/02C12R1/645
Inventor 周呈祥叶伟武同现鹏张繁荣夏文伟仲崇艳凌武海
Owner HANGZHOU ENJOY ENVIRONMENTAL PROTECTION TECH
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