Chitosan-modified glassy carbon electrode-based selective recognition of tryptophan enantiomer containing zinc ion

A technology of tryptophan enantiomers and glassy carbon electrodes, which is applied in the field of electrochemical sensors and molecular recognition, can solve problems such as differences in biological activity, and achieve the effects of simple operation, short time, and easy preparation

Inactive Publication Date: 2015-11-04
CHANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Chiral compounds have the same molecular formula and similar physical and chemical properties, so it is difficult to distinguish them, but their biological activities are very different (such as: pharmacodynamics and pharmacokinetics)

Method used

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  • Chitosan-modified glassy carbon electrode-based selective recognition of tryptophan enantiomer containing zinc ion
  • Chitosan-modified glassy carbon electrode-based selective recognition of tryptophan enantiomer containing zinc ion
  • Chitosan-modified glassy carbon electrode-based selective recognition of tryptophan enantiomer containing zinc ion

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1) Prepare a CS solution (the solvent is 0.1M HCl, pH=1.0), and deposit it on the surface of the glassy carbon electrode with a constant potential, the deposition voltage is -0.5V, and the deposition time is 375s to obtain a non-alkaline treated CS / GCE modified electrode ; The electrode was placed in an alcoholic ammonia solution for alkali treatment for 15 minutes, then rinsed with a small amount of ethanol, and placed in air to dry to obtain an alkali-treated CS / GCE modified electrode.

[0026] (2) The electrode prepared in step 1 was placed in a potassium ferricyanide solution and characterized by cyclic voltammetry. The potential range was -0.2-0.6V, and the scan rate was 0.1V / s.

Embodiment 2

[0028] In order to investigate the differences in the recognition ability of tryptophan enantiomers with different zinc ion concentrations. Therefore, the concentrations of zinc ions were respectively 0.05mM, 0.07mM, 0.1mM, 0.2mM, 0.3mM and 0.4mM to identify tryptophan enantiomers. The results are shown in figure 2 , it can be seen that when the concentration of zinc ions is 0.2mM, the recognition ability is the best. Zn 2+ As a coordinated metal ion in this ternary mixed system, with Zn 2+ As the concentration in L / D-Trp solution increases, more Zn(II) will participate in coordination with CS and L / D-Trp in the solution, and the recognition effect will be significantly improved. When Zn 2+ The best recognition effect was achieved when the concentration of the concentration increased to 0.20mM. But with Zn 2+ As the concentration continues to increase, the interaction between cations increases, which in turn inhibits the Zn complexation reaction. 2+ Activity, which redu...

Embodiment 3

[0030] In order to investigate the differences in the ability of chitosan modified electrodes to recognize different concentrations of tryptophan enantiomers. Therefore, the concentrations of tryptophan were respectively 0.1 mM, 0.3 mM, 0.6 mM, 0.8 mM, 1.0 mM, 1.2 mM and 1.5 mM. See the result image 3 , it can be seen that when the concentration of zinc ions is 1.0mM, the recognition ability is the best.

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Abstract

The invention relates to chitosan-modified glassy carbon electrode-based selective recognition of a tryptophan enantiomer containing zinc ion, comprising the following steps: preparation of alkali-treated chitosan (CS)-modified glassy carbon electrode (GCE) (CS/GCE) and selective recognition of a metal ion-containing tryptophan enantiomer (Zn(II)-L/D-Trp). The invention has beneficial effects as follows: the chitosan-modified electrode is simple and feasible to prepare and the preparation process is environmentally friendly and pollution-free; and the modified electrode has high recognition efficiency of tryptophan enantiomer, has short recognition time and is simple to operate.

Description

technical field [0001] The invention relates to the selective recognition of tryptophan enantiomers containing zinc ions based on a chitosan-modified glassy carbon electrode, and belongs to the field of electrochemical sensors and molecular recognition. technical background [0002] Living systems contain a large number of biomacromolecules, most of which are enantioselective. Chirality is a common phenomenon in nature, such as sugars, amino acids, proteins and DNA are chiral molecules. Chiral compounds have the same molecular formula and similar physical and chemical properties, so it is difficult to distinguish them, but their biological activities are quite different (such as: pharmacodynamics and pharmacokinetics). Therefore, molecular recognition plays a crucial role in distinguishing chiral molecules. At present, there are mainly the following chiral analysis methods, namely: capillary electrophoresis (CE), high performance liquid chromatography (HPLC), fluorescence ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327
Inventor 孔泳鲍丽平陶永新
Owner CHANGZHOU UNIV
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