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A method for detecting the concentration of acrylamide in a solution

A technology of acrylamide and solution is applied in the field of chemical detection to achieve the effects of improving sensitivity, reducing detection cost, and simple and convenient operation.

Active Publication Date: 2017-10-10
深圳市威标检测技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the characteristic single-stranded DNA can be used as a sensing platform to detect acrylamide by electrochemical technology. So far, there are no reports on the use of characteristic single-stranded DNA modified gold electrodes for the detection of acrylamide.

Method used

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  • A method for detecting the concentration of acrylamide in a solution
  • A method for detecting the concentration of acrylamide in a solution

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Experimental program
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Embodiment 1

[0040] An embodiment of the present invention provides a method for detecting the concentration of acrylamide in a solution, comprising:

[0041] A three-electrode system sensor is used to detect acrylamide in the sample solution by differential pulse voltammetry, and the concentration of acrylamide in the sample solution is obtained according to the differential pulse voltammetry curve of acrylamide, wherein, in the three-electrode system The working electrode is a gold electrode modified with a characteristic single-stranded DNA whose sequence is 5'-TTTTTTTTTTGGGGG-(CH 2 ) 6 -SH-3'.

[0042] The present invention uses differential pulse voltammetry to detect acrylamide,

[0043] Differential pulse voltammetry superimposes a continuous pulse with constant amplitude and fixed pulse width on the linear scanning waveform. During the scanning process, the base potential is scanned from the initial potential to the final potential, and the current is sampled before and at the en...

Embodiment 2

[0061] Electrochemical workstation: CHI760E;

[0062] Three-electrode system: the working electrode is a gold electrode modified with characteristic single-stranded DNA, the auxiliary electrode is a platinum electrode, and the reference electrode is an Ag / AgCl electrode;

[0063] Buffer solution: the first phosphate buffer solution with a pH value of 8.0 and a concentration of 0.2mol / L;

[0064] Standard stock solution: 2μmol / L standard acrylamide solution;

[0065] The gold electrodes have been treated before modification: the gold electrodes are placed on the polishing cloth containing the polishing powder with particle size of 1 μm, 0.3 μm, and 0.05 μm in turn to polish to the mirror surface, and then the gold electrodes are placed in acetone, concentration Sonicate 0.5 mol / L sulfuric acid solution and ultrapure water for 1 minute, and rinse with ultrapure water for 0.5 minutes after each ultrasound.

[0066] Modification of gold electrode: drop 7 μL containing 5×10 -6 m...

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Abstract

The invention discloses a three-electrode system to detect acrylamide in the liquid to be detected by differential pulse voltammetry, and obtain the concentration of acrylamide in the liquid to be detected according to the linear equation of acrylamide, wherein the work in the three-electrode system The electrode is a gold electrode modified with a characteristic single-stranded DNA, and the sequence of the characteristic single-stranded DNA is 5'-TTTTT TTTTTGGGGG-(CH2)6-SH-3'. The invention uses differential pulse voltammetry to detect acrylamide with high sensitivity, the detection limit of acrylamide can be reached, the operation is simple, the detection is fast, the sensitivity is high and the selectivity is good, and the method has wide application prospects.

Description

technical field [0001] The invention belongs to the technical field of chemical detection, and in particular relates to a characteristic single-stranded DNA electrochemical sensor for detecting acrylamide concentration by differential pulse voltammetry. Background technique [0002] Acrylamide has mutagenic effects in both in vivo and in vitro tests, and can cause gene mutations and chromosomal abnormalities in mammalian somatic and germ cells, such as micronucleus formation, sister chromatid exchange, polyploidy, aneuploidy, and others Abnormal mitosis, etc., dominant lethal test was positive. It was also proved that the metabolite glycidamide of acrylamide is its main mutagenic active substance. Animal experiments have found that acrylamide can cause tumors in various organs of rats, including mammary gland, thyroid gland, testis, adrenal gland, central nervous system, oral cavity, uterus, and pituitary gland. The International Agency for Research on Cancer (IARC) evalua...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/48G01N27/30
Inventor 肖琦黄珊卢双燕盛家荣黄初升
Owner 深圳市威标检测技术有限公司