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Tissue culture rapid propagation method of tillandsia

A tissue culture, air pineapple technology, applied in the field of plant cultivation, can solve problems such as difficulty in callus, difficulty in sterilizing explants, etc., and achieve the effect of improving the emergence rate

Inactive Publication Date: 2015-11-11
QINGDAO AGRI UNIV
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Problems solved by technology

[0008] The purpose of the present invention is to provide a tissue culture rapid propagation method of Tillandsia pineapple, which solves the problems of difficult explant disinfection and difficult callus induction in the prior art

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  • Tissue culture rapid propagation method of tillandsia

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Embodiment 1

[0024] The tissue culture rapid propagation method of embodiment 1 air pineapple

[0025] 1. Aseptic treatment of seeds: soak the seeds in 70% ethanol solution for 2 minutes, then soak them in 2% sodium hypochlorite solution for 20 minutes, and wash them with sterile water for 3 times. Inoculate in germination medium. After the seeds swell and germinate, the seedlings are placed on the induction medium for treatment.

[0026] Germination formula: 1 / 2MS+0.1mg / LNAA+0.01mg / LKT. Sugar 20g / L, pH 5.8 or so, it is best to build a filter paper bridge to prevent the buds from being excessively soaked in water and necrosis.

[0027] 2. Inducing clustered buds: the culture medium (1000mL) for inducing clustered buds is K / MSvit+ZT1.2mg / L+6-BA2.0mg / L+NAA0.5mg / L, sugar 20g / L, pH6.0 or so, Agar powder 5-10 grams. K / MSvit formula (mg / L): Ammonium sulfate 500; Magnesium sulfate heptahydrate 250; Calcium nitrate tetrahydrate 1000; Dipotassium hydrogen phosphate 250; Manganese sulfate water ...

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Abstract

The invention discloses a tissue culture rapid propagation method of tillandsia. The tissue culture rapid propagation method comprises the following steps: aseptic processing of seeds, induction of multiple shoots, enrichment culture, and culture of strong seedlings, so as to prepare tillandsia strong seedlings. The method solves the problems in the prior art that the explant disinfection and the callus induction are difficult; the growth rate of the tillandsia prepared by adopting the method is increased by 3 times or above as compared with the growth rate of the tillandsia at the natural state; and besides, a bud germinated from one seed can grow into 4-5 seedlings finally, so that the emergence rate is increased by 4 times or above.

Description

technical field [0001] The invention belongs to the technical field of plant cultivation, and in particular relates to a method for tissue culture and rapid propagation of Tillandsia pineapple. Background technique [0002] Air pineapple belongs to bromeliaceae (Bromeliaceae), which is a monocotyledonous plant and a perennial herb. Bromeliaceae has 3 subfamilies, 50 genera, and more than 2,600 species. The three subfamilies are Pitcairnioideae, Tillandsioideae and Bromelioideae. [0003] The vast majority of air pineapple are Tillandsia plants. Tillandsia is the largest genus in Bromeliaceae, with more than 500 native species, distributed in a wide area of ​​1000-3000m above sea level in Latin America. In their native environment, they are mostly epiphytic or hanging from tree trunks or stones, or even cables. [0004] The main organ for absorbing nutrients and water in air pineapple is the "sunflower-like" scale structure covered on the leaf surface. This structure hel...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 李鹏郑桂灵
Owner QINGDAO AGRI UNIV