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A kind of high temperature resistant anaerobic ammonium oxidation sludge cultivation method

An anammox and culture method technology, which is applied in the field of high temperature resistant anammox sludge cultivation, can solve the problems such as no reports of long-term effects of sodium chloride being found, and achieves the promotion of anammox sludge activity, The effect of enhancing heat resistance and broadening application fields

Active Publication Date: 2017-05-03
HANGZHOU NORMAL UNIVERSITY
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  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Before the present invention, do not find that sodium chloride acts on the application report of high-temperature anaerobic ammonium oxidation process for a long time

Method used

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Embodiment 1

[0022] In continuous flow, an upflow anaerobic sludge bed reactor is used, and the sludge concentration at 35°C is 15g L -1 The anaerobic ammonium oxidation granular sludge is inoculated sludge, with a content of 140mg·L -1 The concentration of ammonia nitrogen and nitrite nitrogen is the influent simulated wastewater, the hydraulic retention time is 6h, and the inorganic salt buffer solution to maintain the growth of microorganisms is added and the reaction temperature is gradually increased to adjust the operation of the reactor. The sludge was cultured under the condition of pH 6.87. Wherein the inorganic salt buffer composition used is as follows: KH 2 PO 4 10mg·L -1 , CaCl 2 2H 2 O 7.4mg·L -1 , MgSO 4 2H 2 O 300mg·L -1 , KHCO 3 1250mg·L -1 , the solvent is water. Set up two identical anaerobic reactors as a control group and a test group. The control group is a group without any treatment, and the test group is a group in which a certain concentration of sodi...

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Abstract

The present invention provides a method for culturing anammox sludge with high temperature resistance. The method comprises: adopting an upflow anaerobic sludge blanket reactor, wherein the reactor comprises a water inlet, a water outlet and a reactor cell body; using an anammox granular sludge as seed sludge, and simulated wastewater containing ammonia and nitrite nitrogen as feedwater; adding sodium chloride into the simulated wastewater, and adding an inorganic salt buffer solution that can maintain the growth of microorganisms; gradually raising the reaction temperature to regulate the running of the reactor; culturing in an anaerobic and dark condition, at a temperature of 40-50 DEG C, and with a pH value of 6-7 of feedwater and the hydraulic retention time (HRT) of 4-8 h; and stably running the reactor for 2-5 weeks in a lower temperature range firstly, then running the reactor for 2-5 weeks in a higher temperature range by gradually raising the incubator temperature, so as to run the reactor step by step. The anammox sludge with high temperature resistance cultured by the method according to the invention has high activity and fine granular sludge characteristics, and performance of the reactor is good.

Description

[0001] (1) Technical field [0002] The invention relates to a method for cultivating high-temperature resistant anaerobic ammonium oxidation sludge. [0003] (2) Background technology [0004] As a new biological nitrogen removal process, anaerobic ammonium oxidation is widely favored for its high efficiency and energy saving. The anammox bacteria play an important role in this process. [0005] The hot water used in actual life, the wastewater discharged from many factories and mines, especially power plants, papermaking wastewater, food wastewater, slaughter wastewater, petrochemical wastewater, etc. are discharged at a high temperature, generally greater than 60°C, and the discharge temperature of palm oil wastewater is 80-90°C ℃, plus this type of wastewater directly or indirectly contains high concentrations of ammonia nitrogen. Therefore, the denitrification treatment of this kind of high-temperature wastewater is worthy of attention. If this kind of high-temperature w...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C02F3/28
Inventor 金仁村姬玉欣姜晓燕邓睿王琼芳户海燕
Owner HANGZHOU NORMAL UNIVERSITY