Synchronous Separation of Geniposide and Gardenia Yellow in Multistage Aqueous Two-Phase
A technology of geniposide and gardenia yellow, which is applied in the field of high-yield and high-purity extraction of geniposide and gardenia yellow, can solve the problems of simultaneous extraction of gardenia yellow, improve separation and purification efficiency, and be simple to operate Effect
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Embodiment 1
[0016] At room temperature, prepare 10 g of a two-phase system according to the mass percentage of ammonium sulfate and ethanol at 10%, take 1 ml of the concentrated gardenia crude extract, add it to the two-phase system, and stir with constant temperature magnetic force (20°C, 300r / min) For 40 minutes, the gardenia yellow and geniposide were enriched in the upper phase. Let stand for 60 minutes, and centrifuge at 2000 rpm for 30 minutes to separate the rich phase of geniposide and gardenia yellow. Then add this phase to the lower phase of the same two-phase system, and at room temperature, stir with constant temperature magnetic force (20°C, 300r / min) for 40min, and add 10w.% hydrochloric acid at the same time to make geniposide and gardenia yellow respectively Enriched in the lower phase and the upper phase, the enriched phase of geniposide and gardenia yellow is separated, and the upper phase containing gardenia yellow is concentrated and dried under vacuum rotation at -0.0...
Embodiment 2
[0018] At room temperature, 10 g of a two-phase system was prepared according to the mass percentage of sodium carbonate and isopropanol at 20%. Take 1 ml of the concentrated gardenia crude extract and add it to the two-phase system. min) for 40 min, enrich the gardenia yellow and geniposide in the upper phase. Let stand for 60 minutes, and centrifuge at 2000 rpm for 30 minutes to separate the rich phase of geniposide and gardenia yellow. Then add this phase to the lower phase of the same two-phase system, stir at constant temperature (20°C, 300r / min) for 40min at room temperature, add 30w.% hydrochloric acid to make geniposide and gardenia yellow rich Set in the lower phase and the upper phase, separate the enriched phase of geniposide and gardenia yellow, and the upper phase containing gardenia yellow is concentrated and dried under vacuum rotation at -0.04-0.09MPa, 45°C to obtain gardenia yellow The crude product was crystallized, and then recrystallized from acetone to ob...
Embodiment 3
[0020] At room temperature, 10 g of a two-phase aqueous system was prepared according to the mass percentage of potassium carbonate and isopropanol at 30%, and 1 ml of the concentrated gardenia crude extract was added to the two-phase system, and magnetically stirred at a constant temperature (20°C, 300r / min) for 40 min, enrich the gardenia yellow and geniposide in the upper phase. Let stand for 60 minutes, and centrifuge at 2000 rpm for 30 minutes to separate the rich phase of geniposide and gardenia yellow. Add this phase to the lower phase of the same two-phase system, stir at constant temperature (20°C, 300r / min) for 40min at room temperature, and add 10w.% sulfuric acid to enrich geniposide and gardenia yellow respectively. Collected in the lower phase and the upper phase, the enriched phase of geniposide and gardenia yellow was separated. The upper phase containing gardenia yellow was concentrated and dried under the condition of -0.04-0.09MPa and 45°C in a vacuum to o...
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Abstract
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