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Method for removal of aflatoxin from peanut skin

A technology of aflatoxin and peanut red coat, applied in the direction of organic chemistry, can solve the problem of chemical reaction inactivation of proanthocyanidins, etc., and achieve the effect of ensuring application value, wide source of raw materials, and high purity

Active Publication Date: 2015-11-18
SHANDONG LUHUA GROUP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, for example, patents with application publication numbers CN103880805A, CN103214448A and CN104277025A all record a process for extracting proanthocyanidins from peanut red coats, but all ignore the aflatoxin residues in proanthocyanidins, and subsequent harm to humans It can be seen that the removal of aflatoxin must be considered when extracting proanthocyanidins from peanut red coat
The patent application publication number CN102532085A discloses a method for removing aflatoxin in proanthocyanidin solution with sodium hypochlorite and sodium hydroxide. Although the aflatoxin can be removed, the former is a strong oxidizing agent, and the latter is a strong oxidizing agent. It is a strong base, proanthocyanidins are prone to chemical reactions and inactivation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The method for extracting aflatoxin-free proanthocyanidins from peanut red coats adopts the following process steps:

[0026] 1) Pretreatment and extraction: Dry and crush 200 g of peanut red coat (aflatoxin B1 content 200 μg / kg) through a 60-mesh sieve, add 2 L of ethanol aqueous solution with a concentration of 80% by volume, power 240W, temperature Ultrasonic extraction at 50°C for 20 minutes to obtain an extract containing proanthocyanidins and aflatoxins;

[0027] 2) Filtration and concentration Vacuum suction filtration of the above extract to obtain a clear filtrate, operating at a temperature of 45 ° C and a pressure of -0.08 MPa to obtain a concentrated solution of 150 mL after evaporation;

[0028] 3) Column chromatography Dilute the above concentrated solution with water to 300mL, and then load the sample onto the AB-8 resin column with a diameter-to-height ratio of 1:7, a column volume of 150mL, and maintain a flow rate of 0.35mL / min until the end of sample ...

Embodiment 2

[0032] The method for extracting aflatoxin-free proanthocyanidins from peanut red coats adopts the following process steps:

[0033] 1) Pretreatment and extraction Dry 200g of peanut red coat (aflatoxin B1 content 50μg / kg) and crush it through a 70 mesh sieve, add 3L of ethanol aqueous solution with a concentration of 60% by volume, power 300W, temperature 60 Ultrasonic extraction at ℃ for 10 minutes to obtain an extract containing proanthocyanidins and aflatoxins;

[0034] 2) Filtration and concentration Vacuum suction filtration of the above extract to obtain a clear filtrate, and rotary evaporation at a temperature of 50°C and a pressure of -0.09MPa to obtain a concentrated solution of 150 mL;

[0035] 3) Column chromatography Dilute the concentrated solution with water to 450mL, and then load the sample onto a DM130 resin column with a diameter-to-height ratio of 1:8 and a column volume of 150mL. Keep the flow rate at 0.5mL / min until the sample loading is completed;

[00...

Embodiment 3

[0039] The method for extracting aflatoxin-free proanthocyanidins from peanut red coats adopts the following process steps:

[0040] 1) Pretreatment and extraction Dry and crush 200 g of peanut red coat (aflatoxin B1 content 70 μg / kg) through a 60-mesh sieve, add 2 L of ethanol aqueous solution with a concentration of 70% by volume, power 200W, temperature 50 Ultrasonic extraction at ℃ for 15 minutes to obtain an extract containing proanthocyanidins and aflatoxins;

[0041] 2) Filtration and concentration Vacuum suction filtration of the above extract to obtain a clear filtrate, and rotary evaporation at a temperature of 45 ° C and a pressure of -0.09 MPa to obtain a concentrated solution of 150 mL;

[0042] 3) For column chromatography, dilute the concentrated solution with water to 300mL, and then load the sample onto a DM130 resin column with a diameter-to-height ratio of 1:7, a column volume of 150mL, and maintain a flow rate of 0.3mL / min until the sample loading is comple...

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Abstract

The invention provides a method for removal of aflatoxin from peanut skin. The method includes the steps of: (A) pre-treating peanut skin, then conducting ultrasonic extraction to obtain an extracted liquid, and subjecting the extracted liquid to filtration and pressure reduced distillation to obtain a concentrated solution; (B) adding water into the concentrated solution to perform dilution, then passing the diluted concentrated solution through macroporous resin to conduct chromatography adsorption separation, controlling the speed of the solution flowing through the macroporous resin at 0.3-0.5mL / min, then conduction elution with an ethanol water solution with a volume percentage concentration of 3-40%, controlling the elution flow speed at 3-5mL / min, collecting the eluent and performing evaporation and concentration. The method for removal of aflatoxin from peanut skin provided by the invention has the characteristics of mild operation conditions and low cost, can ensure the quality of the extracted proantho cyanidins and also can well remove the aflatoxin therein, enhances the follow-up application food safety of proantho cyanidins extracted from peanut skin, causes no hazard to human health, and is very suitable for extensive application and promotion.

Description

technical field [0001] The invention relates to the field of biotechnology extraction, in particular to a method for removing aflatoxin from peanut red coat. Background technique [0002] Proanthocyanidins have various physiological and pharmacological activities such as anti-oxidation, anti-aging, and prevention of cardiovascular and cerebrovascular diseases, and are internationally recognized natural antioxidants. It is composed of catechin and epicatechin, including monomers, dimers, trimers, tetramers, pentamers and high polymers, of which dimers to pentamers are called low polyproanthocyanidins. Among various polymers, oligomeric proanthocyanidins have the highest antioxidant activity, and their antioxidant capacity is 50 times that of vitamin E. Because of its high efficiency, low toxicity and high bioavailability, it is widely used in food, beverage, cosmetic and health care products industries. At present, the extraction methods of proanthocyanidins mainly include...

Claims

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Application Information

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IPC IPC(8): C07D311/62
CPCC07D311/62
Inventor 徐会茹杜祖波宫旭洲王珊珊初丽君李秋
Owner SHANDONG LUHUA GROUP
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