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Application of salmeterol in medicine for treating type 2 diabetes and insulin resistance

A technology for insulin resistance and type 2 diabetes, applied in the field of medicine, can solve problems such as liver dysfunction

Inactive Publication Date: 2015-11-25
CHONGQING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since these drugs can cause adverse reactions such as heart failure, edema, and liver dysfunction, it is necessary to study new insulin sensitizer antidiabetic drugs

Method used

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  • Application of salmeterol in medicine for treating type 2 diabetes and insulin resistance
  • Application of salmeterol in medicine for treating type 2 diabetes and insulin resistance
  • Application of salmeterol in medicine for treating type 2 diabetes and insulin resistance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 1. Construction of 3T3-L1 cell insulin resistance model:

[0022] (1) After digesting 3T3-L1 cells in good growth state, use 3*10 4 cells / mL were seeded into 6-well plates at a density of 2 mL / well, and cultured in basal medium. Change the medium once every 36 hours. After changing the medium twice, the cells can reach 90%-100% cell density, and continue to culture with the basal medium to keep the cells in a state of contact inhibition.

[0023] (2) Two days after confluence (contact inhibition) (DAY0), change the cell induction medium and culture for two days, and decide whether to change the medium according to the color of the cell culture medium. Generally, change the culture medium once every 24-36 hours.

[0024] (3) Two days after induction medium 1 culture (DAY2), change to induction medium 2. After two days of culture, decide whether to change the medium according to the color of the cell culture medium. Generally, change the culture medium once every 24-36 h...

Embodiment 2

[0038] 1. Construction of SD rat type 2 diabetes model:

[0039] (1) 80 SPF male SD rats with a body weight of 120 ± 20 grams. After 3 days of trial feeding with the basic rat diet, 7 were randomly selected as the model control group, still fed with the basic rat diet, and the other rats were fed with the basic rat diet. Rats were fed with high-sugar and high-fat rat food, and all rats had free access to tap water. Drinking water was changed every day, feces were cleaned up every two days, and body weight was weighed and recorded every three days.

[0040] (2) After two weeks of feeding, the rats were fasted from food and water, and after fasting for 10 hours, the blood was collected from the tail vein, and the blood glucose of the rats was measured, and the results were recorded. Thereafter, fasting blood glucose was measured once a week, and body weight was still measured and recorded every three days.

[0041] (3) After feeding for 6 weeks, the rats in the model group wer...

Embodiment 3

[0057] 1. Total protein extraction from serum, liver, skeletal muscle and adipose tissue:

[0058] (1) One sample was randomly selected from each animal experimental group, and duplicate wells were set up for each sample, and the preserved serum, liver, skeletal muscle, and adipose tissue samples were taken out from -80°C for later use.

[0059] (2) Take out the animal tissue and put it directly into a mortar placed on an ice tray, add liquid nitrogen, quickly crush and grind the tissue block, add liquid nitrogen and grind again when the tissue is about to become soft, and repeat this three times.

[0060] (3) Grind into powder, weigh 40 mg to a 1.5 mL EP tube, add 400 μL of cell lysate, mix evenly with a shaker and place on ice.

[0061] (4) Oscillate once every 10 minutes, 40 seconds each time, and continue for 6-8 times. During this process, keep the low temperature and keep the protein unchanged.

[0062] (5) After lysing on ice, put it into a low-temperature high-speed r...

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Abstract

The invention provides application of salmeterol in medicine for treating type 2 diabetes and insulin resistance. The salmeterol has effects of lowering blood sugar, resisting diabetes and increasing the cell glucose consumption of a 3T3-L1 insulin resistance model. Measuring of PPAR gamma protein expression quantity through an ELISA method shows that the salmeterol can increase the PPAR gamma protein expression quantity. The salmeterol has evident curative effect on the type 2 diabetes.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to the application of salmeterol in the treatment of insulin resistance, anti-type two diabetes medicine and the medicine for treatment of type two diabetes. technical background [0002] Type 2 diabetes has become the most important type of diabetes, and the affected population is growing rapidly and getting younger. Insulin resistance is the main cause of type 2 diabetes. The insulin sensitizer drugs that mainly treat insulin resistance on the market are mainly thiazolidinedione (TZD) antidiabetic drugs. Thiazolidinedione drugs are antidiabetic drugs that can treat insulin resistance because these compounds are PPARγ receptors. The agonist of PPARγ can regulate the expression of CAP protein after PPARγ activation, and the Cbl / CAP linker protein will activate its downstream proteins Crk, TC10 and other molecules, which will eventually promote the glucose transporter to transport g...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/137A61P3/10A61P5/50
Inventor 舒茂林治华于蕊宰小丽
Owner CHONGQING UNIV OF TECH
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