Saccharomyces cerevisiae chromosome as well as construction method and application thereof

A technology of Saccharomyces cerevisiae and artificial chromosome, applied in the direction of microorganism-based methods, biochemical equipment and methods, using vectors to introduce foreign genetic material, etc., can solve the problem of small number of genes, low product expression, difficult to meet industrial mass production, etc. problem, to achieve the effect of improving the expression
CN105087632AActive Publication Date: 2015-11-25TSINGHUA UNIV +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
TSINGHUA UNIV
Publication Date
2015-11-25

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Abstract

The invention discloses a saccharomyces cerevisiae chromosome as well as a construction method and application thereof. The saccharomyces cerevisiae chromosome comprises two telomeric repeat sequences, two insulator sequences, a centromere-autonomously replicating sequence, a po130 gene and n repeated recombination boxes. Experiment results prove that auxotrophic marker genes of the saccharomyces cerevisiae chromosome are few, normal and stable growth in the situation that screening is not carried out can be realized, the expression quantity and copy number of exogenous genes in yeast are increased, the stability of exogenous genes in yeast is improved, genes integrated into a genome can be increased or reduced, and even expanded into new polygene clusters, and the rapid exogenous expression of beta carotene and violacein is successfully realized in yeast, so that a rapid feasible method is provided for yeast heterogeneous production of various exogenous approaches, and the saccharomyces cerevisiae chromosome can meet the application requirements of fields of gene engineering, metabolic engineering and synthetic biology.
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Description

technical field

[0001] The invention belongs to the fields of genetic engineering, metabolic engineering and synthetic biology, and specifically relates to a Saccharomyces cerevisiae chromosome and its construction method and application; in particular, it relates to a chromosome that can realize multiple genes, multiple metabolic pathways, is expandable, does not require screening, and exists stably Saccharomyces cerevisiae chromosome and its construction method and application. Background technique

[0002] With the development of modern biotechnology and biomedicine, the use of exogenous systems to express and obtain high value-added products is becoming more and more common. Famous successful cases include the fermentation production of artemisinin, etc. Traditional expression systems use microorganisms such as Escherichia coli, Bacillus subtilis, and Saccharomyces cerevisiae, etc., to obtain the desired target by putting enzyme genes that do not exist in these systems,...

Claims

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