An immunomagnetic bead for sorting human cells, its preparation and excision method

Abstract

The invention provides an immunomagnetic bead for sorting human cells and a preparation and cutting-off method of the immunomagnetic bead and particularly relates to a magnetic bead-nucleic acid-antibody compound or a kit containing the magnetic bead-nucleic acid-antibody compound. The compound comprises a magnetic bead, nucleic acid covalently coupled to the magnetic bead and an antibody covalently coupled with the nucleic acid, wherein the nucleic acid comprises at least one enzyme digestion site, and the antibody can specifically bind with antigen on the surfaces of to-be-screened cells. The invention further comprises the purpose and preparation method of the magnetic bead-nucleic acid-antibody compound and a method for using the magnetic bead-nucleic acid-antibody compound to perform cell sorting.

Description

technical field

[0001] The invention belongs to the technical field of medical biology experiments, and relates to an immunomagnetic bead for sorting human cells, its preparation and excision method. Background technique

[0002] Tumor is one of the diseases that seriously endanger human health. Traditional treatment methods such as surgery, radiotherapy, and chemotherapy have been difficult to kill the remaining tumor cells in the body. In the 21st century, with the scientific and technological progress of tumor treatment, the technical system of biotherapy has been developed. Among them, biological therapy is currently the most mature and widely used tumor biological treatment method. Biological therapy is to collect mononuclear cells (PBMC) from the patient's peripheral blood, and then send them to the GMP studio for culture, amplification, induction, and tumor antigen stimulation, so as to obtain DC cells that can recognize cancer cells and have high tumoricidal propert...

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