HPLC method for determination of shikimic acid and 3-dehydroshikimic acid in pomegranate peel

A technology of dehydroshikimic acid and pomegranate peel, applied in the field of medicine, can solve problems such as no relevant reports, and achieve the effects of short elution time, comprehensive detection and high detection efficiency

Active Publication Date: 2016-08-17
SHANDONG INST OF POMOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the method for determining the content of shikimic acid is mainly high-performance liquid chromatography, and there are only a few reports on coniferous plants, star anise and other plants, but there is no relevant report on the method of determining the content of 3-dehydroshikimic acid at home and abroad.

Method used

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  • HPLC method for determination of shikimic acid and 3-dehydroshikimic acid in pomegranate peel
  • HPLC method for determination of shikimic acid and 3-dehydroshikimic acid in pomegranate peel
  • HPLC method for determination of shikimic acid and 3-dehydroshikimic acid in pomegranate peel

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Embodiment 1

[0058] The HPLC assay method of shikimic acid and 3-dehydroshikimic acid in pomegranate peel, comprises the steps:

[0059] (1) Pretreatment of pomegranate fruit samples

[0060] Wash and dry the pomegranate fruit, peel the peel directly into liquid nitrogen and freeze for 5 minutes, then remove it into a petri dish, freeze it in a -20°C refrigerator for 24 hours, and then put it in a freeze dryer for 48 hours; The dried peel is crushed and placed in a -20°C refrigerator for later use;

[0061] (2) preparation of need testing solution

[0062] Weigh 0.1g of the pericarp treated in step (1), put it in a stoppered Erlenmeyer flask, add 40mL of ultrapure water, ultrasonicate for 90min, power 200W, frequency 33kHz; Membrane filtration, stored in a -20°C refrigerator for HPLC determination;

[0063] (3) Content determination of shikimic acid and 3-dehydroshikimic acid

[0064] The experimental conditions of high performance liquid chromatography are as follows: Agilent Zorbax X...

Embodiment 2

[0068] The HPLC assay method of shikimic acid and 3-dehydroshikimic acid in pomegranate peel, comprises the steps:

[0069] (1) Pretreatment of pomegranate fruit samples

[0070] Wash and dry the pomegranate fruit, peel the peel directly into liquid nitrogen and freeze for 10 minutes, then remove it into a petri dish, freeze it in a -20°C refrigerator for 24 hours, and then put it in a freeze dryer for 48 hours; The dried peel is crushed and placed in a -20°C refrigerator for later use;

[0071] (2) preparation of need testing solution

[0072] Weigh 0.1g of the pericarp treated in step (1), put it in a stoppered Erlenmeyer flask, add 40mL of ultrapure water, ultrasonicate for 110min, power 200W, frequency 33kHz; Membrane filtration, stored in a -20°C refrigerator for HPLC determination;

[0073] (3) Content determination of shikimic acid and 3-dehydroshikimic acid

[0074] The experimental conditions of high performance liquid chromatography are as follows: Agilent Zorbax...

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Abstract

The invention discloses an HPLC determination method for shikimic acid and 3-dehydrogenated shikimic acid in pomegranate peels. The method disclosed by the invention comprises the following steps: taking methanol-phosphoric acid water solution with the volume percent of 1% as a mobile phase and carrying out isocratic elution to simultaneously determine the content of shikimic acid and 3-dehydrogenated shikimic acid in the pomegranate peels, so as to pointedly detect shikimic acid and 3-dehydrogenated shikimic acid components in pomegranate fruits. The determination method disclosed by the invention is simple to operate, has short elution time, can detect comprehensively and has high detection efficiency, so that a theoretical foundation is laid for disclosing a metabolism mechanism of gallic acid in the pomegranate fruits.

Description

technical field [0001] The invention relates to an HPLC method for determining shikimic acid and 3-dehydroshikimic acid in pomegranate peel, belonging to the technical field of medicine. Background technique [0002] The fruit of pomegranate (Punica granatum L.) is rich in soluble phenolic substances, steroids and alkaloids and other biologically active substances. Favored by investors, the development prospect is broad. Gallic acid is one of the main phenolic substances in pomegranate fruit, which has a wide range of biological effects and is closely related to its strong health care function. The metabolic pathway of gallic acid has been basically clear. The 3-dehydroshikimic acid generated by the shikimic acid pathway is converted into gallic acid under the action of shikimate dehydrogenase. It is also reported that shikimic acid generates 3-dehydroshikimic acid under the action of shikimate dehydrogenase, and after enolization of 3-dehydroshikimic acid, gallic acid can...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88
Inventor 冯立娟尹燕雷杨雪梅武冲王菲
Owner SHANDONG INST OF POMOLOGY
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