A kind of preparation method of organic/inorganic hybrid quartz capillary monolithic column based on single-stranded dna nucleic acid aptamer modification

Abstract

The invention discloses a preparation method for modifying an organic / inorganic hybrid quartz capillary monolithic column based on a single-stranded DNA nucleic acid aptamer. The steps are as follows: (1) activating the inner surface of the hollow quartz capillary; (2) Weigh CTAB in a centrifuge tube, add TEOS, APTES, absolute ethanol, etc. to obtain an organic / inorganic hybrid monolithic column through multi-step treatment; (3) Rinse with methanol and pure water successively; (4) Activate and react at room temperature for a period of time , washed repeatedly with PBS buffer solution; (5) injecting the ochratoxin A aptamer solution into an organic / inorganic hybrid monolithic column, circulating reaction at room temperature and storing at low temperature to obtain the obtained product. This product is used for highly selective separation and enrichment of trace and ultra-trace ochratoxin, aflatoxin and other substances in complex biological samples and food.

Description

technical field [0001] The invention belongs to the field of chemical analysis and testing instruments, and relates to an organic / inorganic hybrid capillary monolithic column modified based on nucleic acid aptamers, and a preparation method for a novel in-tube solid-phase microextraction monolithic column. The monolithic column is suitable for complex food and biological Rapid, efficient and highly selective separation and enrichment of trace and ultra-trace ochratoxin components in samples. Background technique [0002] Due to the interference of the component to be measured by its coexisting components or due to the limitation of the sensitivity of the determination method itself and the requirements of the state of the component to be measured, most chemical detection and analysis methods require effective and reasonable treatment of the sample in advance. That is, before the analysis and determination, the sample should be physically or chemically treated to extract the ...

AI Technical Summary

Problems solved by technology

[0006] The present invention aims at the problems of easy breakage of SPME, insufficient extraction amount, low selectivity and poor compatibility of biological samples as mentioned above, and combines the characteristics of high affinity, strong specificity and good compatibility of biological samples of nucleic acid aptamers Combined with the advantages of In-tube SPME technology, such as high efficiency, easy to use with instruments, and simple operation, the organic / inorganic hybrid capillary monolithic column is prepared, and the nucleic acid is adapted through the high affinity between streptavidin and biotin A new in-tube SPME extraction column based on nucleic acid aptamer-modified organic / inorganic hybrid quartz capillary monolithic column was developed for trace and ultra-trace ochratoxin in complex biological samples and food Highly selective separation and enrichment of substances such as , aflatoxin, etc.

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