Method for rapidly accumulating lipid by high-temperature stress on micro-algae

A high-temperature stress, high-oil technology, applied in the field of microalgae bioenergy research, can solve the problems of low biomass concentration and achieve the effects of simple operation, increased oil content, and short time consumption

Inactive Publication Date: 2015-12-09
SHANDONG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Previous studies have shown that when Spirulina is cultured at 40°C, its oil content is increased, but

Method used

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  • Method for rapidly accumulating lipid by high-temperature stress on micro-algae

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Embodiment 1

[0027] The invention relates to a microalgae culture method for rapidly accumulating oil under high temperature stress and improving the oil production efficiency of the microalgae. Firstly, the microalgae were cultivated by using the BG11 medium at a temperature of 25° C., an aeration rate of 600 mL / min, and a light intensity of 6000 lux under continuous light conditions. The cultured microalgae is Scenedesmus tetracerus, which comes from the Environmental Science and Engineering Technology Research Center of Shandong Province and has been preserved in the Freshwater Algae Species Bank of the Chinese Academy of Sciences with the preservation number FACHB-1768. After being cultured for 15 days, the microalgae reached the end of the logarithmic growth phase, at which time the biomass concentration of the microalgae reached a maximum of 1.07-1.11 g / L, and the oil content was 23.4%-25.6%. Then place Scenedesmus tetracerida at the end of the logarithmic phase in an environment wit...

Embodiment 2

[0043] The invention relates to a microalgae culture method for rapidly accumulating oil under high temperature stress and improving the oil production efficiency of the microalgae. Firstly, the microalgae were cultivated by using the BG11 medium at a temperature of 20° C., an aeration rate of 500 mL / min, and a light intensity of 5000 lux under continuous light conditions. The cultured microalgae is Scenedesmus tetracerus, which comes from the Environmental Science and Engineering Technology Research Center of Shandong Province and has been preserved in the Freshwater Algae Species Bank of the Chinese Academy of Sciences with the preservation number FACHB-1768. After being cultured for 15 days, the microalgae reached the end of the logarithmic growth phase, at which time the biomass concentration of the microalgae reached a maximum of 1.07-1.11 g / L, and the oil content was 23.4%-25.6%. Then place Scenedesmus tetracerida at the end of the logarithmic phase in an environment wit...

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Abstract

The invention belongs to the field of micro-alga bio-energy research, and relates to a method for rapidly accumulating lipid by high-temperature stress on micro-algae. The micro-algae are cultivated inside a reactor until the micro-algae enter the last stage of the exponential phase and cultivated for 1-2 days in a stress mode in a high-temperature environment, and then high-lipid-content micro-algae are obtained through centrifuging. The method is easy to implement, short in consumed time and high in efficiency, does not affect the biomass concentration of scendesmus quadricauda, is beneficial to increasing the yield of the lipid and can promote the scendesmus quadricauda to rapidly accumulate the lipid. The high-lipid micro-algae produced through the method can be used for extracting gasoline, diesel oil and aircraft fuel and serve as the raw material of a plastic product and medicine.

Description

technical field [0001] The invention belongs to the field of microalgae bioenergy research, and relates to a method for rapidly accumulating oil in microalgae under high temperature stress. Background technique [0002] With the continuous consumption of fossil energy, the continuous emission of carbon dioxide and the intensification of the greenhouse effect, finding new sustainable renewable energy can effectively reduce the total emission of carbon dioxide in the atmosphere. Many renewable biomass have been used to produce biodiesel, including biomass raw materials such as rapeseed, jatropha, palm trees, food waste and microalgae, among which microalgae is a fast-growing and highly adaptable organism , has great nutritional, pharmaceutical and biological value, and can be used as a raw material in some biological and pharmaceutical industries to produce high value-added nutrients, biodiesel and fish bait. Compared with other biomass, microalgae is a kind of biomass with g...

Claims

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Application Information

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IPC IPC(8): C12N1/36C12R1/89
Inventor 裴海燕韩飞胡文容聂昌亮成娟张立杰
Owner SHANDONG UNIV
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