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Pachymaran extracting method

A Poria polysaccharide and extraction method technology, which is applied in the field of extraction of Poria polysaccharide, can solve the problems of complicated extraction process, severe extraction conditions, and high cost of enzymatic hydrolysis, and achieves shortened extraction process time, mild extraction process conditions, and simple extraction process. Effect

Active Publication Date: 2015-12-09
山东迅达康兽药有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The extraction time of hot water extraction is long and the extraction rate of polysaccharide is low; the extraction process of acid-base extraction is cumbersome and the extraction conditions are severe, which can easily destroy the three-dimensional structure of polysaccharide and reduce its biological activity; the cost of enzymatic hydrolysis is high, and only It can act on limited substrates, etc., which limits its wide application

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0016] A kind of extracting method of Poria cocos polysaccharide: carry out as follows: (every part is calculated by 10g)

[0017] (1) After crushing Poria cocos, add 4 times of water to expand, add yeast and lactic acid bacteria at 1% (w / w), the ratio of the two is 1:2, and carry out fermentation. The fermentation temperature is 28 ° C, the pH value is 3.1, and the time is 12 hours;

[0018] (2) Mix the fermented liquid evenly, add compound enzyme preparation, this compound enzyme preparation comprises cellulase, hemicellulase, amylase and pectinase, cellulase, hemicellulase, amylase and pectinase The weight ratio is: 2:2:1:1; the addition amount is 1.2% (w / w), the action temperature is 36°C, the pH value is 3.5, and the time is 10 hours;

[0019] (3) Adding concentration to the reactant after step (2) is 95% ethanol, so that the alcohol content of the reactant reaches 80% (w / w), and the alcohol precipitate is spray-dried to obtain water-soluble pachyrhin 70.1 %about.

Embodiment 2

[0021] (1) After pulverizing Poria cocos, add 5 times of water to expand, add yeast and lactic acid bacteria at 3% (w / w) for fermentation, the weight ratio of yeast and lactic acid bacteria is 1:2, the fermentation temperature is 30°C, and the pH value 3.5, time 14 hours;

[0022] (2) Mix the fermented liquid evenly, add compound enzyme preparation, this compound enzyme preparation comprises cellulase, hemicellulase, amylase and pectinase, cellulase, hemicellulase, amylase and pectinase The weight ratio is: 2:2:1:1; the addition amount is 2% (w / w), the action temperature is 39°C, the pH value is 4, and the time is 11 hours;

[0023] (3) Adding concentration to the reactant after step (2) is 95% ethanol, so that the alcohol content of the reactant reaches 80% (w / w), after which the alcohol analyte is spray-dried to obtain water-soluble pachymansin 70.2 %.

Embodiment 3

[0025] A kind of extracting method of Poria cocos polysaccharide: it is characterized in that carry out according to the following steps: (every part is calculated by 10g)

[0026] (1) After pulverizing Poria cocos, add 6 times of water to expand, add yeast and lactic acid bacteria at 5% (w / w), the ratio of the two is 1:2, and carry out fermentation. The fermentation temperature is 31 ° C, the pH value is 4.0, and the time is 16 hours;

[0027] (2) Mix the fermented liquid evenly, add compound enzyme preparation, this compound enzyme preparation comprises cellulase, hemicellulase, amylase and pectinase, cellulase, hemicellulase, amylase and pectinase The weight ratio is: 2:2:1:1; the addition amount is 2.5% (w / w), the action temperature is 42°C, the pH value is 4.2, and the time is 12 hours;

[0028] (3) Adding concentration to the reactant after step (2) is 95% ethanol, so that the alcohol content of the reactant reaches 80% (w / w), and the alcohol precipitate is spray-dried ...

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Abstract

The invention relates to a pachymaran extracting method. The method comprises the steps of 1, smashing poria cocos and then adding the smashed poria cocos to 4-6 times water to be expanded, and adding saccharomycetes and lactic acid bacteria based on 1-5% (w / w) according to the ratio of 1:2 for fermentation, wherein fermentation temperature is 28-31 DEG C, PH is 3.1-4.0 and time is 12-16 h; 2, evenly mixing a fermentation broth, and adding a complex enzyme preparation which contains cellulose, hemicellulase, amylase and pectinase based on 1.2-2.5% (w / w), wherein reaction temperature is 36-42 DEG C, PH is 3.5-4.2, and time is 10-12 h; 3, conducting reactant alcohol precipitation with alcohol, adding alcohol to enable the alcohol content of a reactant to reach 80% (w / w), and conducting spray-drying on an alcohol precipitation substance to obtain about 70.2% water-soluble pachymaran. The method has the advantages that extraction technique is simple, extraction time is shortened, extraction conditions are mild, the obtained pachymaran is high in content and biological activity, and the effect is remarkable compared with the existing extraction technique.

Description

technical field [0001] The invention relates to the technical field of polysaccharide extraction, in particular to a method for extracting pachyrhin with simple process, short time and mild conditions. Background technique [0002] Poriacocos (Schw.) Wolf is the dry sclerotium of Polyporaceae fungus, which is a traditional Chinese medicine. It began to be recorded in the classical medical work "Shen Nong's Materia Medica", and was included in the "Chinese Pharmacopoeia" of our country's previous editions. function, anti-aging, anti-allergic, anti-tumor, anti-inflammatory and other biological activities. Poria cocos polysaccharide is widely used, and it is a large variety of traditional Chinese medicine that can not only prevent and treat diseases, but also maintain health and strengthen the body. [0003] In recent years, the extraction of Poria cocos polysaccharide generally has hot water extraction method, alkali solution extraction method, acid extraction method, enzyma...

Claims

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Application Information

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IPC IPC(8): C12P19/14C12P19/04C08B37/00
Inventor 汪安国刘文利王尚明
Owner 山东迅达康兽药有限公司
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