Ganoderma lucidum fermentation protoplasm cosmetic and preparation method and application thereof
A technology of Ganoderma lucidum fermentation and cosmetics, applied in the biological field, can solve problems such as damaging the environment, achieve the effects of simplifying production steps, saving production costs, and maintaining natural activity
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Embodiment 1
[0033] Embodiment 1, the preparation of ganoderma lucidum fermented protoplasm
[0034] 1. Activation of strains
[0035] Pick a ring of wine yeast (Saccharomyces ellipsoideus) colony in the liquid medium, put it into a shaker to activate the strain, and obtain the activated wine yeast liquid.
[0036] 2. Purification of strains
[0037] The activated bacterial solution obtained in step 1 was serially diluted and plated to obtain a single colony to obtain purified wine yeast.
[0038] 3. Expansion and cultivation of strains
[0039] The purified bacterial classification obtained in step 2 is inoculated into YPD medium (pH value is 7.0), cultivated in a shaker at 25° C., and when the OD value=0.6-1.0, the fermented bacterial liquid is obtained (the bacterial classification at the In the logarithmic phase, the concentration is 10 6 -10 8 CFU / ml).
[0040] 4. Obtaining the original puree of Ganoderma lucidum fermentation
[0041] Inoculate 22mL of the fermented bacteria li...
Embodiment 2
[0042] Embodiment 2, ganoderma lucidum fermentation protoplasm as the application of cosmetics:
[0043] 1. Properties of Ganoderma lucidum fermented puree:
[0044] The appearance of the Ganoderma lucidum fermented puree (supernatant) prepared in this example is viscous liquid, and the color is light yellow to brownish yellow. The pH value is 5.2-6.3, the viscosity is 200-500cP, the soluble solid content is 1.5-5.0%, the total number of colonies is less than 50CFU / ml, and no pathogenic bacteria are detected. According to the hygienic standard GB7916-87 for cosmetics, the total number of bacteria in cosmetics is not higher than 1000CFU / ml, so this fermented puree meets the quality requirements of cosmetics.
[0045] The component analysis of the Ganoderma lucidum fermented puree was carried out. The detection method of protein was referred to GB5009.5-2010; the detection method of crude polysaccharide was referred to GB / T5009.8-2008; the detection method of flavonoids was ref...
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