Golden camellia polysaccharide extracting method

An extraction method, the technology of golden camellia, applied in the direction of drug combination, allergic diseases, etc., can solve the problem of low immune activity and achieve the effects of enhancing immune activity, reducing viscosity, and improving water solubility

Active Publication Date: 2015-12-16
GUILIN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although the above-mentioned literature discloses that Camellia polysac

Method used

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  • Golden camellia polysaccharide extracting method

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Embodiment 1

[0021] 1) Grinding the dried leaves of Camellia japonica, adding water 20 times its weight, and extracting twice by reflux, each extraction for 1 hour, combining the extracts, filtering, and the filtrate is the water extract to obtain the water extract;

[0022] 2) Concentrate the water extract to an extract with a relative density of 1.1 at 60°C, add 90v% ethanol until the alcohol content is 80v%, let it stand for precipitation, suction filter, and take the precipitate for later use;

[0023] 3) the precipitate of step 2) is washed with dehydrated alcohol and acetone successively, and the consumption of dehydrated alcohol and acetone is 2 times of the weight of the precipitate, then add distilled water to dissolve, and the consumption of distilled water is 2 times of the precipitate weight after washing. times, and then add protease of 0.3% of the dry weight of golden camellia tea leaves, and enzymolysis time is 1 to 3 hours at 40°C to inactivate the enzyme to obtain an enzymo...

Embodiment 2

[0027] 1) Grinding the dried leaves of Camellia japonica, adding water 30 times its weight, reflux extraction 3 times, each extraction for 3 hours, combining the extracts, filtering, the filtrate is the water extract to obtain the water extract;

[0028] 2) Concentrate the water extract to an extract with a relative density of 1.3 at 60°C, add 95v% ethanol until the alcohol content in the mixed solution is 85v%, let it stand for precipitation, suction filter, and take the precipitate for later use;

[0029] 3) the precipitate of step 2) is washed with dehydrated alcohol and acetone successively, and the consumption of dehydrated alcohol and acetone is 4 times of the precipitate weight, then add distilled water to dissolve, and the consumption of distilled water is 4 times of the precipitate weight after washing. times, and then add 3% protease of the dry weight of golden camellia tea leaves, enzymolysis time is 3h at 60°C, the enzyme is inactivated, and the enzymolysis solution...

Embodiment 3

[0033] 1) Pulverize the golden camellia tea leaves, add water 25 times its weight, reflux extraction 2 times, extract 2 hours each time, combine the extracts, filter, and the filtrate is the water extract to obtain the water extract;

[0034] 2) Concentrate the water extract to an extract with a relative density of 1.2 at 60°C, add 90v% ethanol until the alcohol content in the mixed solution is 80v%, let it stand for precipitation, suction filter, and take the precipitate for later use;

[0035] 3) the precipitate of step 2) is washed with dehydrated alcohol and acetone successively, and the consumption of dehydrated alcohol and acetone is 3 times of the precipitate weight, then add distilled water to dissolve, and the consumption of distilled water is 3 times of the precipitate weight after washing times, and then add 1.5% protease of the dry weight of golden camellia tea leaves, enzymolysis time is 2h at 50°C, the enzyme is inactivated, and the enzymolysis solution is obtaine...

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Abstract

The invention provides a golden camellia polysaccharide extracting method. The golden camellia polysaccharide extracting method golden camellia polysaccharide extracting method comprises the following steps that 1, golden camellia leaves are subjected to extraction with water to obtain a water extract solution; 2, after the water extract solution is concentrated, ethanol is added to perform precipitation, and a precipitate is obtained for standby application; 3, the precipitate obtained in the step 2 is washed by using absolute ethyl alcohol and acetone sequentially, then distilled water is added for dissolution, and then protease is added to perform enzymolysis so as to obtain enzymatic hydrolysate; 4, the enzymatic hydrolysate is processed under frequency ultrasonic waves, dialysis is performed by using a dialysis bag capable of cutting off 18000-28000 molecular weight, the retained liquid is subjected to dialysis by using a dialysis bag capable of cutting off 400000-500000 molecular weight, the obtained permeated liquid is concentrated, then ethanol is added for precipitation, and a precipitate is obtained for standby application; 5, the precipitate obtained in the step 4 is washed by using absolute ethyl alcohol and acetone sequentially, then distilled water is added for dissolution, and activated carbon decoloration, filtration and drying are performed to obtain golden camellia polysaccharide. The golden camellia polysaccharide prepared by means of the golden camellia polysaccharide extracting method has higher immunological competence.

Description

technical field [0001] The invention belongs to the technical field of biological extraction, and in particular relates to an extraction method of camellia japonica polysaccharide. Background technique [0002] Camelliachrysantha (Hu)Tuyama belongs to the plant of Camellia genus Camellia in the family Theaceae, and is known as the "Queen of the Tea Clan" and "The Giant Panda in the Plant Kingdom". As a traditional Chinese herbal medicine of the Zhuang nationality in Guangxi, golden camellia tea has various physiological functions such as lowering blood sugar, lowering blood fat, inhibiting tumor growth, and improving the body's immunity. Among them, golden camellia polysaccharide is considered to be one of the important functional components. "Studies on Anti-immune Liver Injury Effects of Camellia Camellia Polysaccharides" (published in West China Pharmaceutical Journal, 2014, 9(5): 525-527) discloses that the extraction method of Camellia camellia polysaccharides is as fol...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61P37/04
Inventor 邹登峰陈素一张小玲黄晓
Owner GUILIN MEDICAL UNIVERSITY
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