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Method for determining five effective components in zedoary oil by using gas chromatography

A technology of gas chromatography and active ingredients, which is applied in the field of determination of the content of five active ingredients in zedoary oil, can solve the problem of inability to separate detection limits, and achieve reliable implementation, good separation effect, and easy implementation

Inactive Publication Date: 2015-12-16
湖北荆楚理工资产经营管理有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The technical problem to be solved by the present invention is that because the components in the volatile oil are complex, and the molecular structure conformation of the active ingredients in the zedoary oil is similar, the defects of inseparability and high detection limit are likely to occur when measuring with HPLC method, and provide Simultaneous Determination of Five Active Components in Volatile Oil by a Gas Chromatographic Method

Method used

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  • Method for determining five effective components in zedoary oil by using gas chromatography
  • Method for determining five effective components in zedoary oil by using gas chromatography

Examples

Experimental program
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Effect test

Embodiment 1

[0015] Example 1 , Stability, precision and recovery detection of the content determination of five components in zedoary oil:

[0016] Weigh 10.00 mg of standard substance of gemmacone, elemene, furadienes, curcumol, and curdione, dissolve it in ethanol and set the volume to 10.00 mL to obtain a standard stock solution with a concentration of 1 mg / mL, and then further use ethanol to Dilute 50 times as the standard solution, and then dilute to 10, 50, 100.0, 500.0μg / mL standard solution.

[0017] Chromatographic column: TR-1, 30m (length) 0.25mm (inner diameter) 0.25μm (film thickness);

[0018] Injection port temperature: 250°C;

[0019] Split ratio: 40:1;

[0020] Injection volume: 10.0μL;

[0021] Column temperature: 50°C (hold 0min); increase temperature at 20°C / min to 100°C (hold 0min), 4°C / min to 250°C (hold 5min);

[0022] Flow rate: 1.00mL / min;

[0023] Detector: hydrogen flame ionization detector (FID);

[0024] Detector temperature: 250°C;

[0025] 1. Stabili...

Embodiment 2

[0032] Example 2 , Determination of five active ingredients in zedoary oil samples from different sources

[0033] Seven batches of zedoary oil samples from different sources were taken to determine the contents of gemmaconone, elemene, furadienes, curcumol and curcumedione under the above-mentioned chromatographic conditions.

[0034]

[0035] Visible by above-mentioned example, method of the present invention is applicable to the content determination of five kinds of active ingredients in zedoary oil, those of ordinary skill in the art can carry out content determination to five kinds of active ingredients in zedoary oil by this method, has certain production practice.

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Abstract

The invention provides a method for determining five effective components in zedoary oil by using gas chromatography. The method comprises the following steps: A, weighing five chemical substances, i.e., germacrone, elemene, furanodiene, cincumol and curdione, then dissolving the five chemical substances with ethanol, then weighing the obtained solutions, using the solutions as mass concentration stock solutions, further diluting the solutions to obtain a series of standard working solutions with concentration gradients and detecting the standard working solutions and a sample treating solution by using a gas chromatograph; and B, contrasting the chromatogram of the detected sample treating solution with the chromatograms of the standard working solutions so as to obtain the contents of the five effective components, i.e., germacrone, elemene, furanodiene, cincumol and curdione. The invention has the following advantage: the method is rapid and effective in detection of the contents of the five effective components in zedoary oil.

Description

technical field [0001] The invention relates to a method for measuring the contents of five active ingredients in zedoary oil, in particular to a method for measuring the contents of five active ingredients in zedoary oil by gas chromatography. Background technique [0002] The technical problem to be solved by the invention is to overcome the defect that the low response value of volatile oil in other instruments causes the detection limit to be on the high side. In order to achieve the above object, the present invention takes the following design scheme: The method for determining the contents of the five components in the oil comprises the following steps. Contents of the invention [0003] The technical problem to be solved by the present invention is that because the components in the volatile oil are complex, and the molecular structure conformation of the active ingredients in the zedoary oil is similar, the defects of inseparability and high detection limit are li...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 彭佩顾薇施红杨成雄杨希雄
Owner 湖北荆楚理工资产经营管理有限公司
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