Method for preparing trichophyton scanning electron microscope sample

Abstract

The invention provides a method for preparing a trichophyton scanning electron microscope sample. When a sample is fixed, the sample is sequentially treated by at least two kinds of glutaraldehyde solution with different concentration, and the concentration of the latter glutaraldehyde is higher than that of the former glutaraldehyde. Virulent osmic acid is not used in the method, and the glutaraldehyde with gradient concentration is innovatively used for multiple times of fixing, so the fixing effect is good, the method is safe and convenient, and a new method is provided for preparing the trichophyton scanning electron microscope sample.

Description

technical field [0001] The invention relates to a preparation method of a trichophyton scanning electron microscope sample, belonging to the field of biotechnology. Background technique [0002] The pretreatment of samples is an essential process for electron microscope observation, and the quality of the treatment effect is directly related to the results of electron microscope observation. [0003] Since the hyphae of Trichophyton are lumpy and some are thick, there is a phenomenon of insufficient contact with the reagent during the sample processing process, resulting in uneven sample processing, and the results cannot be accurately judged. Through many repeated experiments, it is found that the most important step in the electron microscope sample preparation process is fixation. If the fixation effect is not good, the follow-up treatment cannot play a good role in improving it. [0004] The most commonly used method is glutaraldehyde-osmic acid (osmium tetroxide) doubl...

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Problems solved by technology

[0003] Since the hyphae of Trichophyton are lumpy and some are thick, there is a phenomenon of insufficient contact with the reagent during the sample processing process, resulting in uneven sample processing, and the results cannot be accurately judged

Through repeated experiments, it was found that the most important step in the electron microscope sample preparation process is fixation. If the fixation effect is not good, the follow-up treatment cannot play a good role in improving it.

[0004] The most commonly used method is glutaraldehyde-osmic acid (osmium tetroxide) double immobilization at present, but osmic acid is highly toxic, and contact or inhalation can cause headache, respiratory tract inflammation, eye disease, bronchial disease, pneumonia, etc. Health poses a serious potential threat, and it is necessary to study a low-toxicity fixation method to replace the traditional double fixation method

There are also studies using glutaraldehyde alone for fixation, but it is easy to shrink the bacteria, and the effect is not satisfactory.

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