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Biomass carrier immobilized bacteria method for treating chlorinated organic waste water

A technology of chlorinated organic matter and biomass carrier, which is applied in the field of biological sciences to achieve the effect of low processing cost, convenient material collection, and efficient synergistic processing

Inactive Publication Date: 2017-06-16
WUHAN INSTITUTE OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The problem to be solved by the present invention is to provide a method for effectively treating waste water that is simple in process, low in cost, low in energy consumption and suitable for industrial production for conventional pollutants in industrial and domestic waste water

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0028] The preparation process of biomass carrier immobilized bacteria is as follows:

[0029] 1) The strains come from the soil at 9-11 cm below the surface, and are first expanded and cultured at 20-30 °C, pH neutral, and liquid PDA medium for 3-5 days to obtain bacterial suspension A; the liquid PDA medium is fresh potato 200 -250g / L(H 2 O), sucrose 15-25g / L (H 2 O), urea 10-15g / L (H 2 O).

[0030] 2) Cultivate the bacterial suspension A at 20-30° C. and pH neutral condition, and cultivate the liquid selective medium for 7-10 days to obtain the wood-rot fungus bacterial suspension; the liquid selective medium is (NH 4 ) 2 SO 4 5-10g / L(H 2 O), K 2 HPO 4 2-4g / L(H 2 O), NaH 2 PO 4 2-4g / L(H 2 O), MgSO 4 .7H 2 O0.1-0.2g / L (H 2 O), chloroform wastewater 200mL / L (H 2 O), co-substrate rice husk 1-2(m)% / L(H 2 O), benomyl 10-20mg / L (H 2 O), streptomycin sulfate 400-600mg / L (H 2 O), o-phenylphenol 0.005-0.008(m)% / L(H 2 O), Tween-800.5mL / L (H 2 O).

[0031] 3) Ino...

Embodiment 1

[0034] Take 100ml of waste water (mainly containing chlorinated organic pollutants) after equipment washing in the production process of a coolant manufacturer. The water quality is 6650mg / L of COD. Add 2-5 immobilized particles to the water phase liquid 90ml, keep the temperature at 30°C, pH neutral, and continue to aerate. After 3 days of reaction, take the supernatant and measure the COD to be 36mg / L, and the COD removal rate is 99.45% .

Embodiment 2

[0036] Take 100ml of wastewater produced by the coolant manufacturer (mainly containing chlorinated organic pollutants), dilute it by a certain number of times, and the water quality is 11610mg / L COD, adjust the pH, add 50ml of extraction agent, shake, and after static layering, take out 80ml of the aqueous phase liquid , adding 2-5 immobilized particles to it, keeping the temperature at 25°C, neutral pH, and continuous aeration. After reacting for 3 days, take the supernatant and measure the COD to be 95mg / L, and the COD removal rate is 99.18%.

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Abstract

The invention discloses a method for treating chlorinated organic compound wastewater by virtue of biomass carrier immobilized bacteria. The method comprises the following steps: selecting soil from the location which is 9cm-11cm below the ground surface layer as a strain, carrying out enlarged culture for 3-5 days under liquid-state PDA culture medium conditions, so as to obtain bacterium suspension A; culturing a liquid state by virtue of a culture medium for 7-10 days to obtain wood decay fungi suspension; culturing a solid state by virtue of a culture medium for 3-5 days to obtain a pure wood decay fungi strain; mixing extracted and filtered chlorinated organic compound wastewater with rice husks, carrying out high-temperature steam sterilization, inoculating the pure wood decay fungi strain, and carrying out shake culture for 5-7 days, so as to obtain immobilized microorganisms; and adding the immobilized microorganisms into chlorinated organic compound wastewater, and reacting for 2-3 days, so as to obtain purified wastewater. In the system, the rice husks serve as a biological carrier and simultaneously supply a carbon source for microorganisms, so that the cost for independently feeding the carbon source to industrial wastewater is saved. Furthermore, the rice husks, being of a wood decay fungi, are suitable for being used as the carbon source.

Description

technical field [0001] The invention relates to the technical field of biological sciences, in particular to a method for treating waste water with bacteria immobilized on biomass carriers. Background technique [0002] Sewage biological treatment technology has become the leading technology in the field of sewage treatment. As an economical, reasonable and effective water pollution control engineering method, it plays an important role in improving the quality of water environment and preventing water pollution. [0003] At present, the immobilization methods of microorganisms mainly include surface adsorption immobilization method, cross-linking immobilization method, embedding immobilization method and so on. The cross-linking immobilization method is to modify the surface of the carrier, increase the surface active groups of the carrier, and use two or more functional groups to directly carry out cross-linking reactions with the surface groups of microorganisms to form c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C02F3/34C02F101/36
Inventor 张莉高永宝高菲汪志丁瑶
Owner WUHAN INSTITUTE OF TECHNOLOGY
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