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Preparation method of taxifolin

A technology of taxifolin and extract, applied in the field of natural medicinal chemistry, can solve the problems of long separation and purification cycle, complicated operation and high production cost, and achieve the effect of shortening the separation and purification cycle, being beneficial to industrial production and simplifying the operation.

Inactive Publication Date: 2015-12-23
SUZHOU HEYAN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] For this reason, what the present invention is to solve is the technical problem that the existing taxifolin preparation method has higher production cost, longer separation and purification cycle, and more complicated operation, thereby proposing a method with lower production cost and shorter separation and purification cycle , the preparation method of the easier taxifolin

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  • Preparation method of taxifolin

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Effect test

Embodiment 1

[0028] The present invention provides a kind of preparation method of taxifolin, comprises the following steps:

[0029] (1) Pulverize and sieve the leaves of Huangqi, take 10g of Huangqi leaves, add 10L of 30% ethanol solution for reflux extraction, and concentrate the extract under reduced pressure to obtain extract A;

[0030] (2) The extract A is purified by D101 macroporous resin, using ethanol as mobile phase A, water as mobile phase B, gradient elution, the steps of gradient elution are: A:B volume ratio is 0%:100 %, A:B volume ratio is 10%:90%, A:B volume ratio is 40%:60%, A:B volume ratio is 50%:50%, HPLC detection, collection, and extract B;

[0031] (3) The extract B is dissolved in ethanol containing 1% acetic acid, stirred and hydrolyzed at 80° C. to obtain hydrolyzate C;

[0032] (4) The hydrolyzate C was neutralized to pH 5-6 by adding 0.01M phosphate buffer solution, the neutralization solution was concentrated under reduced pressure, allowed to stand at room ...

Embodiment 2

[0036] The present invention provides a kind of preparation method of taxifolin, comprises the following steps:

[0037] (1) Pulverize and sieve the leaves of Huang Qi, take 10 g of Huang Qi leaves, add 2L of 90% ethanol solution for ultrasonic extraction, and concentrate the extract under reduced pressure to obtain extract A;

[0038] (2) The extract A is purified by HPD-100 macroporous resin, with ethanol as mobile phase A, water as mobile phase B, gradient elution, the step of gradient elution is: A:B volume ratio is 0% : 100%, A:B volume ratio is 10%:90%, A:B volume ratio is 40%:60%, A:B volume ratio is 50%:50%, HPLC detection, collection, to obtain extract B;

[0039] (3) The extract B is dissolved in water containing 8% benzoic acid, stirred and hydrolyzed at 100° C. to obtain a hydrolyzate C;

[0040] (4) The hydrolyzate C was neutralized to pH 5-6 by adding 0.05M sodium hydroxide solution, the neutralization solution was concentrated under reduced pressure, allowed to...

Embodiment 3

[0043] The present invention provides a kind of preparation method of taxifolin, comprises the following steps:

[0044] (1) Pulverize and sieve the leaves of Huang Qi, take 10 g of Huang Qi leaves, add 6L of 40% ethanol solution for infiltration and extraction, and concentrate the extract under reduced pressure to obtain extract A;

[0045] (2) The extract A is purified through AB-8 macroporous resin, with ethanol as mobile phase A, water as mobile phase B, gradient elution, the steps of gradient elution are: A:B volume ratio is 0% : 100%, A:B volume ratio is 10%:90%, A:B volume ratio is 40%:60%, A:B volume ratio is 50%:50%, HPLC detection, collection, to obtain extract B;

[0046] (3) The extract B is dissolved in an ethanol solution containing 5% succinic acid, stirred and hydrolyzed at 90°C to obtain a hydrolyzate C;

[0047] (4) The hydrolyzate C was neutralized to pH 5-6 by adding 0.03M sodium bicarbonate solution, the neutralization solution was concentrated under redu...

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Abstract

The invention belongs to the field of natural medicinal chemistry and specifically relates to a preparation method of taxifolin. The preparation method comprises the following steps: taking 10 parts by weight of Engelhardia roxburghiana Wall leaf, adding 2-10 parts by volume of a 30-90% ethanol solution to extract, and carrying out vacuum concentration on an extract to obtain an extract product A; purifying through macroporous resin, using ethanol as a mobile phase A and using water as a mobile phase B to carry out gradient elution, detecting by HPLC, and collecting to obtain an extract product B; dissolving in 1-8% organic acid-containing ethanol and / or water, and stirring and hydrolyzing at 80-100 DEG C to obtain a hydrolysate C; neutralizing to pH5-6 by adding 0.01-0.05M of alkali liquor, carrying out vacuum concentration on the neutralization solution, standing at room temperature for 5-24 h, filtering, and collecting filter cake to obtain a precipitate D; and recrystallizing to obtain taxifolin. The relation of part by weight and part by volume is g / L. According to the invention, organic acid used for acid hydrolysis is optimized such that acid used for acid hydrolysis is recycled and reused. Therefore, corrosion to equipment is reduced, and production cost is decreased. By optimizing steps after acid hydrolysis, the separation and purification period is shortened and operations are simplified, thus being more beneficial to industrial production.

Description

technical field [0001] The invention belongs to the field of natural medicinal chemistry, and in particular relates to a preparation method of taxifolin. Background technique [0002] Taxifolin is one of the active ingredients of natural flavonoids, which has many important biological activities, and can inhibit and activate various enzymes, thereby producing different physiological effects. Because it contains more phenolic hydroxyl groups, it has a strong antioxidant effect. Not only can effectively eliminate excess free radicals from the human body, promote capillary permeability, effectively restore capillary elasticity, improve immune function, prevent inflammation and tumor formation, and reduce the occurrence of cancer; but also can inhibit DGAT enzyme and MTP Enzyme activity reduces apolipoprotein B secretion to lower triglycerides and cholesterol in serum and hepatocytes. Clinically, Taxifolin has been used in the prevention and treatment of cerebral thrombosis, s...

Claims

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Application Information

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IPC IPC(8): C07D311/32C07D311/40
CPCC07D311/32C07D311/40
Inventor 夏增华曾鸣
Owner SUZHOU HEYAN BIOTECH
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