Egg yolk antibody extraction method suitable for large-scale industrial production
A technology of egg yolk antibody and extraction method, which is applied in the direction of immunoglobulin from eggs, etc., can solve the problems of high cost, no biological toxic and side effects, etc., and achieve the effect of low cost and simple steps
Inactive Publication Date: 2015-12-23
DALIAN UNIV OF TECH
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Under acidic and low ionic strength conditions, octanoic acid can combine with most of the yolk proteins to form irreversible precipitates, only IgY antibody proteins remain in the supernatant and do not react with it, and octanoic acid has a very low solubility in water, at 20°C the solubility is 0.68g / L, the residual amount after extraction is very small. Therefore, octanoic acid has been widely used in the medical industry and has no toxic and side effects on organisms. However, the entire extraction process requires the use of centrifuges and the addition of a large amount of octanoic acid. Considering the economy of industrial production problem, its cost is too high
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Embodiment 1
[0032] 1. Strain: Vibrio splendidus
[0033] 2. Vaccine preparation: Inoculate the above-mentioned strains in 2216E liquid culture at 28°C to the logarithmic phase. When the count is 108cfu / mL, collect the bacterial suspension in a sterilized centrifuge tube, centrifuge at 5000r / min, and discard the supernatant solution, resuspend the pellet 3 times with phosphate buffer solution, add 1% formaldehyde solution to inactivate, mix with Freund's complete adjuvant or Freund's incomplete adjuvant to make a vaccine.
[0034] 3. Immunization of laying hens: immunize laying hens with vaccines (Laihang laying hens, 120 days old, 20 feathers, isolated with commercial feed).
[0035] 4. Collect immunized eggs, separate egg yolks and egg whites, dilute the separated egg yolks with 4, 5, 6, and 7 times the volume of water, adjust the pH to 5.0 with hydrochloric acid, let stand overnight, and centrifuge at 3000r / min After 10 minutes, take the supernatant, measure its fat removal rate, prote...
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The invention discloses an egg yolk antibody extraction method suitable for large-scale industrial production. The method comprises steps as follows: egg yolk is separated from immunized eggs and evenly stirred, and an egg yolk liquid is obtained; distilled water with the volume 5-10 times that of the egg yolk liquid is added to the egg yolk liquid, and the pH value is adjusted to range between 5.0 and 5.2; the egg yolk liquid is unfrozen at the temperature of 4 DEG C after being frozen at the temperature of subzero 20 DEG C for a night; freezing and unfreezing are repeated for 1-3 times, centrifugation is performed, and a supernatant is obtained. The method suitable for large-scale industrial extraction of egg yolk antibodies has the characteristics that the steps are convenient, the cost is low, and the method is efficient, environment-friendly and pollution-free.
Description
technical field [0001] The invention relates to a method for extracting egg yolk antibody on a large scale in industry, in particular to an economical, convenient and green method for extracting egg yolk antibody. Background technique [0002] Eggyolkantibodies (IgY) refer to the immunoglobulins present in poultry yolk. Because of its good therapeutic effect, high specificity, safety and no residue, no drug-resistant bacteria, and a large amount of preparation, the process Advantages such as simplicity stand out in the boom in research on alternatives to antibiotics. Since it was first discovered by Klemperer in 1898, it has been widely used in the prevention and treatment of livestock and poultry diseases and disease diagnosis. The U.S. Food and Drug Administration (FDA) lists yolk antibodies as "Generally Recognized As Safe (GRAS)" and regards yolk antibodies as Nutraceuticals. However, egg yolk is rich in nutrients and is a good medium for bacterial growth, which is eas...
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IPC IPC(8): C07K16/02
Inventor 李晓宇靖凯霖徐永平王丽丽李淑英
Owner DALIAN UNIV OF TECH