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Method for harvesting dunaliella salina by using waste spirulina culture liquid

A technology of spirulina and salina, which is applied in the field of harvesting salina by using spirulina culture waste liquid, can solve the problems of high cost, low flocculation efficiency, poor performance, etc., and achieve the effect of reducing production cost and reducing production cost

Inactive Publication Date: 2015-12-23
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Positive starch or modified chitosan is still unable to be used on a large scale in industrialization because of the high cost (1.15USD / kgbiomass, Yu-ShenCheng et al.2011), and it does not perform well in seaweed and denser algae liquid. Medium flocculation efficiency is 80% smaller
Alkaline flocculation still has some shortcomings, such as Na after flocculation + , Ca 2+ 、K + iso-medium non-nutritive metal ion accumulation, and mainly contains Mg 2+ loss of nutrient ions, which in turn is not conducive to recycling the medium

Method used

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  • Method for harvesting dunaliella salina by using waste spirulina culture liquid
  • Method for harvesting dunaliella salina by using waste spirulina culture liquid
  • Method for harvesting dunaliella salina by using waste spirulina culture liquid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 1. Take 50ml of spirulina liquid that has been cultivated to the logarithmic phase, centrifuge it in a centrifuge at 4000rpm for 5min, take 20ml of the supernatant and add it to 80ml of salina algae liquid, stir at 150rpm for 3min, let stand for 4h, every hour Determination of absorbance OD 630 , the determination formula is (OD 630初始 -OD 630测定 ) / OD 630初始 , the maximum recovery rate is 75.26%. The algae liquid cultured for 7-10 days can be used, and the pH of the algae liquid is in the alkaline range.

[0021] 2. Take 20ml of fresh spirulina culture medium and add it to 80ml of salina algae liquid, stir for 3min with a magnet at 150rpm, as a comparative experiment, let it stand for 5h, and measure the absorbance OD every hour 630 , the determination formula is (OD 630初始 -OD 630测定 ) / OD 630初始 , the maximum recovery rate is 66.99%.

[0022] 3. Take the supernatant after flocculation in step 1 and fresh medium according to the ratio of 85:0, 2:15, 4:13, 8:9, 9:8, 13...

Embodiment 2

[0024] 1. Take 50ml of spirulina liquid that has been cultivated to the logarithmic phase, centrifuge it in a centrifuge at 4000rpm for 5min, take 17ml of the supernatant and add it to 83ml of salina liquid, stir with a magnet at 150rpm for 3min, let it stand for 3h, every hour Determination of absorbance OD 630 , the determination formula is (OD 630初始 -OD 630测定 ) / OD 630初始 , the maximum recovery rate is 74.77%.

[0025] 2. Take 17ml of fresh spirulina medium and add it to 83ml of salina algae liquid, stir for 3min at 150rpm with a magnet, let stand for 5h, measure the absorbance OD every hour 630 , the determination formula is (OD 630初始 -OD 630测定 ) / OD 630初始 , the maximum recovery rate is 65.83%.

Embodiment 3

[0027] 1. Take 50ml of the spirulina liquid that has been cultivated to the logarithmic phase, centrifuge it in a centrifuge at 4000rpm for 5min, take 12.5ml of the supernatant and add it to 87.5ml of the salina algae liquid, stir it at 150rpm for 3min, and let it stand for 5h. Measure the absorbance OD every hour 630 , the determination formula is (OD 630初始 -OD 630测定 ) / OD 630初始 , the maximum recovery rate is 73.59%.

[0028] 2. Take 12.5ml of fresh spirulina medium and add it to 87.5ml of salina algae liquid, stir for 3min with a magnet at 150rpm, let it stand for 5h, and measure the absorbance OD every hour 630 , the determination formula is (OD 630初始 -OD 630测定 ) / OD 630初始 , the maximum recovery rate is 58.16%.

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Abstract

The invention discloses a method for harvesting dunaliella salina by using a waste spirulina culture liquid, and relates to a dunaliella salina harvesting method. The method comprises the following steps: 1) collecting supernate after spirulina is harvested, and testing the pH value; 2) adding the supernate collected in the step 1) into dunaliella salina, leaving to stand, and testing the harvesting rate; and 3) mixing the supernate obtained in the step 2) with a dunaliella salina culture medium, inoculating dunaliella salina cells, performing circulation culture, and testing the growth curve of dunaliella salina. By making full use of characteristics that the pH of the culture liquid used in the growth process of spirulina is alkali and dunaliella salina can be automatically flocculated under a high pH value, the supernate of spirulina can be sufficiently utilized, the supernate obtained after dunaliella salina is harvested can be continuously adopted for dunaliella salina culture, and the production cost is low. The harvested dunaliella salina is free of organic test agent or toxic or harmful substance, influence to the quality of an algae paste product in the harvesting process can be avoided, the waste supernate can be sufficiently utilized, and secondary pollution can be prevented.

Description

technical field [0001] The invention relates to a method for harvesting salina, in particular to a method for harvesting salina by using spirulina culture waste liquid. Background technique [0002] As a renewable biological resource, microalgae is of great significance in solving the current energy and environmental problems caused by excessive reliance on fossil energy. The use of microalgae biomass to produce renewable fuels or chemicals is a solution to energy shortages and carbon dioxide emissions. An effective way to over-emit greenhouse gases. However, microalgae as aquatic organisms grow in water with a low biological density, and the harvesting of microalgae in general large-scale culture often becomes one of the main bottlenecks in subsequent processing. Harvesting costs account for 20% to 30% of the cost of microalgae refining. Conventional microalgae harvesting methods include centrifugation, filtration, flocculation, air suspension, etc. (see patent CN20091020...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12R1/89
Inventor 贾立山熊琼陈弘俊陈长平刘广发高亚辉
Owner XIAMEN UNIV
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