Novel culture medium good for increasing yield of lactic acid bacteria
A culture medium and lactic acid bacteria technology, applied in the direction of microorganism-based methods, bacteria, microorganisms, etc., can solve the problems of cumbersome preparation of lactic acid bacteria culture medium and low number of viable bacteria, and achieve the effects of shortening the production cycle, increasing the density of lactic acid bacteria, and increasing production
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[0019] 1 Materials and methods
[0020] 1.1 Materials
[0021] The strain KLDS2.000 lactic acid bacteria was introduced from the Key Laboratory of Dairy Science and Education Department of a university.
[0022] Medium Medium 1Z n SO 4 ·7H 2 O0.30—0.40g, MgSO 4 ·7H 2 O0.10-0.18g, peptone 5.0-5.5g, beef extract 5.0-6.0g, tryptone 10.0-11.0g, yeast extract powder 5.0-6.0g, glucose 10.0-12.0g, Tween-801.0-1.5ml, K 2 HPO 4 1.5-2.5g, sodium acetate 4.5-5.5g, diammonium hydrogen citrate 1.5-2.5g, 0.5% fructooligosaccharide, 1% CaCO 3 Add distilled water to 1000ml.
[0023] Medium 2 Soy tryptone 16.7g, casein peptone 8.3g, lactose 10g, yeast extract powder 5g, add distilled water to 1000ml.
[0024] Medium 3 Casein peptone 10g, yeast extract powder 2.5g, glucose 5g, soybean peptone 5g, Tween-801.0ml, L-cysteine hydrochloride 0.5g, mixed salt solution 5ml, distilled water 995ml, mixed salt solution: K 2 HPO 2 0.0g, MgCl 2 ·6H 2 O5.0g, ZnSO 4 ·7H 2 O2.5g, CaCl 2 1.5g...
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