Novel culture medium good for increasing yield of lactic acid bacteria

A culture medium and lactic acid bacteria technology, applied in the direction of microorganism-based methods, bacteria, microorganisms, etc., can solve the problems of cumbersome preparation of lactic acid bacteria culture medium and low number of viable bacteria, and achieve the effects of shortening the production cycle, increasing the density of lactic acid bacteria, and increasing production

Inactive Publication Date: 2015-12-23
CHENGDU YICHUANGSI BIOLOGICAL SCI & TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The new culture medium is a kind of lactic acid bacteria, which can effectively solve the problems of tedious preparation of the culture medium of lactic acid...

Method used

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  • Novel culture medium good for increasing yield of lactic acid bacteria
  • Novel culture medium good for increasing yield of lactic acid bacteria
  • Novel culture medium good for increasing yield of lactic acid bacteria

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Experimental program
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Embodiment 1

[0019] 1 Materials and methods

[0020] 1.1 Materials

[0021] The strain KLDS2.000 lactic acid bacteria was introduced from the Key Laboratory of Dairy Science and Education Department of a university.

[0022] Medium Medium 1Z n SO 4 ·7H 2 O0.30—0.40g, MgSO 4 ·7H 2 O0.10-0.18g, peptone 5.0-5.5g, beef extract 5.0-6.0g, tryptone 10.0-11.0g, yeast extract powder 5.0-6.0g, glucose 10.0-12.0g, Tween-801.0-1.5ml, K 2 HPO 4 1.5-2.5g, sodium acetate 4.5-5.5g, diammonium hydrogen citrate 1.5-2.5g, 0.5% fructooligosaccharide, 1% CaCO 3 Add distilled water to 1000ml.

[0023] Medium 2 Soy tryptone 16.7g, casein peptone 8.3g, lactose 10g, yeast extract powder 5g, add distilled water to 1000ml.

[0024] Medium 3 Casein peptone 10g, yeast extract powder 2.5g, glucose 5g, soybean peptone 5g, Tween-801.0ml, L-cysteine ​​hydrochloride 0.5g, mixed salt solution 5ml, distilled water 995ml, mixed salt solution: K 2 HPO 2 0.0g, MgCl 2 ·6H 2 O5.0g, ZnSO 4 ·7H 2 O2.5g, CaCl 2 1.5g...

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Abstract

The invention discloses a novel culture medium good for increasing yield of lactic acid bacteria. The medium comprises 0.30-0.40 g/l of ZnSO4.7H2O, 0.10-0.18 g/l of MgSO4.7H2O, 5.0-5.5 g/l of peptone, 5.0-6.0 g/l of beef extract, 10.0-11.0 g/l of tryptone, 5.0-6.0 g/l of yeast extract powder, 10.0-12.0 g/l of glucose, 1.0-1.5 ml/l of tween 80, 1.5-2.5 g/l of K2HPO4, 4.5-5.5 g/l of sodium acetate, 1.5-2.5 g/l of diammonium hydrogen citrate, 0.5% of fructooligosaccharide, and 1% of CaCO3. The novel culture medium is capable of increasing the yield of lactic acid bacteria, the problems such as preparation complexity and low viable count can be effectively solved, the viable count is increased, stable production can be achieved, and the yield can be increased.

Description

technical field [0001] The invention relates to the technical field of biological culture medium, and specifically refers to a novel culture medium that is beneficial to improving the yield of lactic acid bacteria. Background technique [0002] Lactic acid bacteria are important physiological beneficial bacteria in human and animal intestines. The reported physiological functions of lactic acid bacteria include: adjusting intestinal flora, preventing constipation, reducing the incidence of colon cancer, producing antibiotics, enhancing the immune system, increasing protein and Vitamin metabolism, alleviating lactose intolerance, treating liver damage, anti-tumor, lowering cholesterol, etc. With the continuous accumulation of beneficial research results on lactic acid bacteria and the continuous improvement of modern people's requirements for healthy diet, there is an upsurge of research and development of food, health care products and medicines containing lactic acid bacter...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/225
Inventor 周洁
Owner CHENGDU YICHUANGSI BIOLOGICAL SCI & TECH
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