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Bacterial colony chromogenic medium capable of achieving autoclaved sterilization and preparing method and application thereof

A chromogenic medium and autoclaving technology, applied in biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of inability to prepare plate count agar, failure of TTC color reaction, and inability to co-exist, and achieve Easy to count, low cost, and easy to operate

Inactive Publication Date: 2015-12-23
厦门欧凯科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, TTC cannot be directly added to the medium and sterilized together with the medium, otherwise the medium will turn brown after sterilization and cause the TTC color reaction to fail, and thus cannot be used in experiments
[0005] Chinese patent application CN103725744A discloses a chromogenic medium, in addition to essential nutrients such as agar, TTC and Tween 80 have also been added, but Tween 80 is an oily substance that cannot coexist with other powdery substances such as agar, Therefore it cannot be prepared as final marketable plate count agar

Method used

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  • Bacterial colony chromogenic medium capable of achieving autoclaved sterilization and preparing method and application thereof
  • Bacterial colony chromogenic medium capable of achieving autoclaved sterilization and preparing method and application thereof
  • Bacterial colony chromogenic medium capable of achieving autoclaved sterilization and preparing method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1: the preparation method and component content determination of colony chromogenic medium

[0018] In a large number of preliminary tests, the range of the added amount of sucrose fatty acid ester (SE-11) was finally reduced to 0.005g-0.020g, and 2,3,5-triphenyltetrazolium chloride (TTC) was added The range of the amount is reduced to 0.005g-0.020g, and the test is carried out in increments of 0.005g to determine the final sucrose fatty acid ester (SE-11) and 2,3,5-triphenyltetrazolium chloride (TTC) The amount added and the composition of the colony chromogenic medium are determined.

[0019] The test is as follows:

[0020] Culture medium preparation:

[0021] The first group, the medium contains 23.5g of commercially available plate count agar per liter, 0.005g of sucrose fatty acid ester (SE-11), 0.005g of 2,3,5-triphenyltetrazolium chloride (TTC) ; After weighing the above components, dissolve in distilled water in no order, boil to dissolve, adjust ...

Embodiment 2

[0051] Embodiment 2: Preparation of colony chromogenic medium

[0052] The medium contains 23.5g of commercially available plate count agar per liter, 0.005g-0.015g of sucrose fatty acid ester (SE-11), 0.005g- 2,3,5-triphenyltetrazolium chloride (TTC) 0.015g;

[0053] After weighing the above-mentioned components, dissolve them in distilled water in no order, boil to dissolve, adjust the pH value to 7.0±0.2, and autoclave them for later use. The autoclaving conditions are: 115°C, 15min.

Embodiment 3

[0055] Control medium (that is, the plate count agar medium specified in the national standard GB4789.2-2010 "Determination of the total number of colonies in food microbiological examination of national food safety standards"): Dissolve 23.5 g of commercially available plate count agar in 1000 mL of distilled water , heat to dissolve, adjust the pH to 7.0±0.2, and prepare for use after autoclaving. The autoclaving conditions are: 115°C, 15min.

[0056] Experimental group: obtained in Example 2.

[0057] Sample dilution: Weigh 25.0g of xanthan gum (food additive monomer, colloid, powder) and place it in a sterile homogenizing cup filled with 225mL of normal saline, homogenize at 10000r / min for 1min, and make a weight-to-volume ratio of 1:10 homogeneous sample solution. Use a 1mL sterile micropipette to draw 1mL of the homogeneous sample solution obtained, slowly pour it along the tube wall into a sterile test tube containing 9mL of diluent (note that the tip of the pipette or...

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Abstract

The invention discloses a bacterial colony chromogenic medium capable of achieving autoclaved sterilization and the preparing method and application thereof. Each liter of the medium contains 23.5 g market plate count agar, 0.005-0.015 g sucrose fatty acid ester SE-11 and 0.005-0.015 g 2,3,5-triphenyltetrazolium chloride. The medium can be used for measuring the number of bacterial colonies in food and food additives, bacterial colonies are red or light red, boundaries are clear, bacterial colony color development is obvious, counting is easy, and the medium can be popularized to actual testing to facilitate reagent testing.

Description

technical field [0001] The invention relates to the field of bacterial colony written identification, in particular to an autoclavable colony chromogenic medium, a preparation method and an application. Background technique [0002] The total number of colonies refers to the total number of bacterial colonies grown per gram (per milliliter) of the sample under certain conditions (such as aerobic conditions, nutritional conditions, pH, incubation temperature and time, etc.). The determination of the total number of bacterial colonies is used to determine the degree of food contamination by bacteria and the hygienic quality. It reflects whether the food meets the hygienic requirements during the production process, so as to make an appropriate hygienic evaluation of the tested samples. The total number of colonies indicates the quality of food hygiene to a certain extent. [0003] At present, the medium used in the domestic standard inspection method for the total number of c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/06
Inventor 蒋东晋范婷婷洪春梅孙金玉
Owner 厦门欧凯科技有限公司
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