A rice fermented puree cosmetic and its preparation method and application
A technology of fermenting puree and cosmetics, applied in the biological field, can solve problems such as kidney function damage and product color darkening, and achieve the effects of simplifying production steps, realizing mass production, and saving production costs
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Embodiment 1
[0032] Embodiment 1, a kind of preparation method of rice fermentation protoplasm
[0033] 1. Activation of strains
[0034] Pick a ring of Bifidobacterium bifidum (Zygosaccharomycesrouxii) colony in the liquid medium, put it into a shaker to activate the strain, and obtain the activated Bifidobacterium bifidum bacterial liquid.
[0035] 2. Purification of strains
[0036] The activated bacterial solution obtained in step 1 was serially diluted and plated to obtain a single colony to obtain purified Bifidobacterium bifidum.
[0037] 3. Expansion and cultivation of strains
[0038] Inoculate the purified bacterial classification obtained in step 2 into the YPD medium (pH value is 7.0), cultivate in a shaker at 32°C, and when the OD value=0.6-1.2, obtain the fermented bacteria liquid (the bacterial classification In the logarithmic phase, the concentration is 10 6 -10 9 CFU / ml, pH 6.5-7.5).
[0039] 4. Obtaining rice fermented puree
[0040] Inoculate 30mL of the fermenta...
Embodiment 2
[0041] Embodiment 2, the application of fermented rice puree as cosmetics
[0042] 1. Properties of rice fermented puree
[0043] The appearance of the fermented extract prepared in Example 1 is viscous liquid, and the color is light white to light yellow. The pH value is 5.2-6.3, the viscosity is 200-500cP, the soluble solid content is 1.5-5.0%, the total number of colonies is less than 50CFU / ml, and no pathogenic bacteria are detected. According to the hygienic standard GB7916-87 for cosmetics, the total number of bacteria in cosmetics is not higher than 1000CFU / ml, so this fermented extract meets the quality requirements of cosmetics.
[0044] The composition analysis of the rice fermented puree is carried out. The protein detection method refers to GB5009.5-2010; the crude polysaccharide detection method refers to GB / T 5009.8-2008; the flavonoid detection method refers to GB / T 5009.124-2003; The results of T8313-2008 are as follows:
[0045] The fermented rice puree pre...
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