Plant seed mutagenesis method and device

A plant seed and seed technology, applied in the field of plant seed mutagenesis and plant seed mutagenesis device, can solve the problems of drug action and uneven cleaning, unstable mutation, inaccurate processing time, etc., to achieve thorough removal of mutagen, Correspondence and time-specific effects

Active Publication Date: 2016-01-06
YUNNAN ACAD OF TOBACCO AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The seed mutagenesis before the present invention is all done manually, and there are the following technical problems: 1) The seed mutagen is a strong carcinogenic agent, which is harmful to people; cause mutagenesis failure; 3) the treatment time is not accurate, the mutation rate is often unstabl

Method used

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  • Plant seed mutagenesis method and device
  • Plant seed mutagenesis method and device
  • Plant seed mutagenesis method and device

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0039] Example one

[0040] Treat China Tobacco 100

[0041] 1) Use 50% bleach to treat for 3-6 minutes;

[0042] 2) Rinse and bleach ingredients;

[0043] 3) At room temperature, 0.50% EMS ethyl methanesulfonate treated tobacco seeds for 12 hours.

[0044] 4) Rinse 8 times.

[0045] The result: 500 grams of seeds were treated, and 20,000 seeds were selected with a germination rate of about 95%, 17,380 seedlings were grown, 10759 were planted, and 5647 seeds were obtained, and the mutation rate was 1-3 mutation sites / kb.

Example Embodiment

[0046] Embodiment two

[0047] Process Yunyan 87

[0048] 1) Use 50% bleach water to treat for 6 minutes, centrifuge and filter to dry;

[0049] 2) After adding the rinse agent, centrifuge for 1 minute to remove the bleach components;

[0050] 3) At room temperature, soak tobacco seeds in deionized water for 10-12 hours, and then centrifuge and filter to dry;

[0051] 4) At room temperature, treat tobacco seeds with 0.30% EMS ethyl methanesulfonate for 12 hours, and then centrifuge and filter to dry;

[0052] 5) After adding the rinsing agent, rotate the drum for 1 minute, centrifuge and filter dry, and repeat the rinsing a total of 8 times.

[0053] The result: 200 grams of seeds were treated, and 20,000 seeds were selected with a germination rate of about 75%, 13090 seedlings were grown, 10812 were planted, and 2347 seeds were obtained, and the mutation rate was 10-13 mutation sites / kb.

Example Embodiment

[0054] Embodiment three

[0055] Process K326

[0056] 1) Use 10% bleach water to treat for 12 minutes, centrifuge and filter to dry;

[0057] 2) After adding the rinse agent, centrifuge for 1 minute to remove the bleach components;

[0058] 3) At room temperature, soak tobacco seeds in deionized water for 10-12 hours, and then centrifuge and filter to dry;

[0059] 4) At room temperature, treat tobacco seeds with 0.75% EMS ethyl methanesulfonate for 12 hours, and then centrifuge and filter to dry;

[0060] 5) After adding the rinsing agent, rotate the drum for 1 minute, centrifuge and filter dry, and repeat the rinsing a total of 8 times.

[0061] Results: Treating 50 grams of seeds, selecting 20,000 seeds with a germination rate of about 55%, 9700 seedlings, 7965 plants, 1765 seeds obtained, and the mutation rate is 20-25 mutation sites / kb.

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Abstract

The invention discloses a plant seed mutagenesis method and device. The method provided by the invention includes the steps of: rinsing; cleaning; soaking in deionized water; mutagen treatment; and mutagen removal. The device provided by the invention includes: a box, a roller and a driving motor. The box is provided with the roller, the roller is connected to the driving motor, the roller is in connection with a water supply pipe and a water outlet pipe, the water supply pipe is provided with a treatment agent pipe that is communicated with a treatment agent box, and the treatment agent pipe is equipped with a feeding gate valve, which is connected to an automatic controller. According to the invention, the driving motor drives the roller, the roller rotates to stir a seed treatment liquid so as to conduct mutagenesis on seeds. The water supply pipe communicated with the roller is connected to the treatment agent pipe, the treatment agent pipe is provided with the feeding gate valve, and the automatic controller controls the feeding gate valve, the roller and other components so as to adapt to each step of seed mutagenesis and realize full automation of the seed mutagenesis treatment process. Without the need of long-term artificial operation, the method and the device reduce the labor intensity, and guarantee that the technical staff can avoid mutagen damage.

Description

technical field [0001] The invention belongs to the technical field of agricultural seed breeding, and in particular relates to a plant seed mutagenesis method and a plant seed mutagenesis device with simple structure, convenient operation and easy observation. Background technique [0002] The seed mutagenesis before the present invention is all done manually, and there are the following technical problems: 1) The seed mutagen is a strong carcinogenic agent, which is harmful to people; cause mutagenesis failure; 3) the treatment time is not accurate, the mutation rate is often unstable, and it is impossible to analyze, improve and improve the mutagenesis technique; 4) a large number of mutagenic plant seeds often have uneven chemical action and cleaning, and mutation Unstable, unable to perform molecular biology screening analysis on mutants, leading to failure of seed breeding projects. Therefore, it is of great practical significance to develop a mutagenesis method for p...

Claims

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Application Information

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IPC IPC(8): A01H1/06
Inventor 李文正王丙武宋中邦高玉龙许美玲李永平张轲张家瑞
Owner YUNNAN ACAD OF TOBACCO AGRI SCI
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