Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Plant seed mutagenesis method and device

A plant seed and seed technology, applied in the field of plant seed mutagenesis and plant seed mutagenesis device, can solve the problems of drug action and uneven cleaning, unstable mutation, inaccurate processing time, etc., to achieve thorough removal of mutagen, Correspondence and time-specific effects

Active Publication Date: 2016-01-06
YUNNAN ACAD OF TOBACCO AGRI SCI
View PDF2 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The seed mutagenesis before the present invention is all done manually, and there are the following technical problems: 1) The seed mutagen is a strong carcinogenic agent, which is harmful to people; cause mutagenesis failure; 3) the treatment time is not accurate, the mutation rate is often unstable, and it is impossible to analyze, improve and improve the mutagenesis technique; 4) a large number of mutagenic plant seeds often have uneven chemical action and cleaning, and mutation Unstable, unable to perform molecular biology screening analysis of mutants, leading to failure of seed breeding projects

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Plant seed mutagenesis method and device
  • Plant seed mutagenesis method and device
  • Plant seed mutagenesis method and device

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Treat China Tobacco 100

[0041] 1) Use 50% bleach for 3-6 minutes;

[0042] 2) rinsing and bleaching water components;

[0043] 3) At room temperature, tobacco seeds were treated with 0.50% EMS ethyl methanesulfonate for 12 hours.

[0044] 4) Rinse 8 times.

[0045] The effect produced: 500 grams of seeds were treated, 20,000 seeds were selected, the germination rate was about 95%, 17,380 seedlings were planted, 10,759 plants were planted, 5,647 seeds were obtained, and the mutation rate was 1-3 mutation sites / kb.

Embodiment 2

[0047] Treat Yunyan 87

[0048] 1) Use 50% bleaching water to treat for 6 minutes, then centrifuge and filter to dry;

[0049] 2) After adding the rinse agent, 1 minute, centrifuge and dry to remove the bleaching water component;

[0050] 3) At room temperature, soak tobacco seeds in deionized water for 10-12 hours, then centrifuge and dry;

[0051] 4) At room temperature, tobacco seeds were treated with 0.30% EMS ethyl methanesulfonate for 12 hours, and then dried by centrifugation;

[0052] 5) After adding the rinsing agent, rotate the drum for 1 minute, centrifugally filter and dry, and repeat rinsing 8 times in total.

[0053] The effect produced: 200 grams of seeds were treated, 20,000 seeds were selected, the germination rate was about 75%, 13,090 seedlings were grown, 10,812 plants were planted, 2,347 seeds were obtained, and the mutation rate was 10-13 mutation sites / kb.

Embodiment 3

[0055] Process K326

[0056] 1) Treat with 10% bleaching water for 12 minutes, then centrifuge and filter to dry;

[0057] 2) After adding the rinse agent, 1 minute, centrifuge and dry to remove the bleaching water component;

[0058] 3) At room temperature, soak tobacco seeds in deionized water for 10-12 hours, then centrifuge and dry;

[0059] 4) At room temperature, tobacco seeds were treated with 0.75% EMS ethyl methanesulfonate for 12 hours, and then dried by centrifugation;

[0060] 5) After adding the rinsing agent, rotate the drum for 1 minute, centrifugally filter and dry, and repeat rinsing 8 times in total.

[0061] The effect produced: 50 grams of seeds were treated, 20,000 seeds were selected, the germination rate was about 55%, 9,700 seedlings were planted, 7,965 plants were planted, and 1,765 seeds were obtained. The mutation rate was 20-25 mutation sites / kb.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a plant seed mutagenesis method and device. The method provided by the invention includes the steps of: rinsing; cleaning; soaking in deionized water; mutagen treatment; and mutagen removal. The device provided by the invention includes: a box, a roller and a driving motor. The box is provided with the roller, the roller is connected to the driving motor, the roller is in connection with a water supply pipe and a water outlet pipe, the water supply pipe is provided with a treatment agent pipe that is communicated with a treatment agent box, and the treatment agent pipe is equipped with a feeding gate valve, which is connected to an automatic controller. According to the invention, the driving motor drives the roller, the roller rotates to stir a seed treatment liquid so as to conduct mutagenesis on seeds. The water supply pipe communicated with the roller is connected to the treatment agent pipe, the treatment agent pipe is provided with the feeding gate valve, and the automatic controller controls the feeding gate valve, the roller and other components so as to adapt to each step of seed mutagenesis and realize full automation of the seed mutagenesis treatment process. Without the need of long-term artificial operation, the method and the device reduce the labor intensity, and guarantee that the technical staff can avoid mutagen damage.

Description

technical field [0001] The invention belongs to the technical field of agricultural seed breeding, and in particular relates to a plant seed mutagenesis method and a plant seed mutagenesis device with simple structure, convenient operation and easy observation. Background technique [0002] The seed mutagenesis before the present invention is all done manually, and there are the following technical problems: 1) The seed mutagen is a strong carcinogenic agent, which is harmful to people; cause mutagenesis failure; 3) the treatment time is not accurate, the mutation rate is often unstable, and it is impossible to analyze, improve and improve the mutagenesis technique; 4) a large number of mutagenic plant seeds often have uneven chemical action and cleaning, and mutation Unstable, unable to perform molecular biology screening analysis on mutants, leading to failure of seed breeding projects. Therefore, it is of great practical significance to develop a mutagenesis method for p...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A01H1/06
Inventor 李文正王丙武宋中邦高玉龙许美玲李永平张轲张家瑞
Owner YUNNAN ACAD OF TOBACCO AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com