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Application of anemoside B4 used as immunomodulator to drugs for treating acute inflammations

An immunomodulator, Pulsatilla saponin technology, applied in the directions of drug combinations, medical preparations containing active ingredients, antipyretics, etc., to achieve safe and reliable toxic and side effects, no toxic and side effects.

Active Publication Date: 2016-01-06
刘琦
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Modern pharmacological studies have found that Pulsatilla saponins have the effects of enhancing immune function, anti-inflammation, anti-tumor, and anti-pathogenic microorganisms, so they have received extensive research and attention, but there are no reports of Pulsatilla saponin monomer compounds with anti-inflammatory activity

Method used

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  • Application of anemoside B4 used as immunomodulator to drugs for treating acute inflammations
  • Application of anemoside B4 used as immunomodulator to drugs for treating acute inflammations
  • Application of anemoside B4 used as immunomodulator to drugs for treating acute inflammations

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Blood biochemical detection of Pulsatilla saponin B4 on the indicators of acute liver inflammation in mouse serum after LPS stimulation.

[0034] The ICR mice with a body weight of 18-22g were randomly divided into 6 groups, 10 in each group, which were blank control group, LPS modeling group (10mg / kg), LPS+positive control drug group (dexamethasone, 5mg / kg) ), LPS+Pulsatilla saponin B4 low dose group (50mg / kg), LPS+Pulsatilla saponin B4 medium dose group (100mg / kg), LPS+Pulsatilla saponin B4 high dose group (200mg / kg). B4 treatment was given 3 hours before LPS modeling, and positive control drug and Pulsatilla saponin B4 were given 3 hours and 6 hours after modeling, and blood was collected from the eyeball after 3 hours of treatment. The whole blood taken from the mouse was left at room temperature for 2 hours, centrifuged at 3500 rpm / min for 15 minutes, and 200 μl of the supernatant was taken to detect biochemical indicators related to liver injury with a blood bioch...

Embodiment 2

[0038] Effects of Pulsatilla saponin B4 on acute inflammatory response and lung injury induced by LPS:

[0039] The ICR mice with a body weight of 18-22g were randomly divided into 6 groups, 10 in each group, which were blank control group, LPS modeling group (10mg / kg), LPS+positive control drug group (dexamethasone, 5mg / kg) ), LPS+Pulsatilla saponin B4 low dose group (50mg / kg), LPS+Pulsatilla saponin B4 medium dose group (100mg / kg), LPS+Pulsatilla saponin B4 high dose group (200mg / kg). B4 treatment was given immediately after LPS modeling, B4 was given for treatment 3 hours after modeling, positive drug dexamethasone was given 1 hour before modeling, and blood was taken from the eyeball 6 hours after modeling (take blood vessels and add anticoagulant ), dissected, separated the whole lung of each mouse, randomly took three mouse lungs and put them in 10% formaldehyde buffer solution to dehydrate them. The fixed tissues were then embedded in paraffin, sectioned, stained with ...

Embodiment 3

[0044] Effects of Pulsatilla saponin B4 on LPS-induced acute kidney injury:

[0045] BALB / C mice with a body weight of 18-22g were randomly divided into 7 groups, 10 in each group, respectively blank control group, LPS modeling group (10mg / kg), LPS+positive control drug group (dexamethasone, 5mg / kg), LPS+Pulsatilla saponin B4 low-dose group (50mg / kg), LPS+Pulsatilla saponin B4 medium-dose group (100mg / kg), LPS+Pulsatilla saponin B4 high-dose group (200mg / kg), B4 high-dose group (200mg / kg kg). B4 treatment was given 3 hours before LPS modeling, and positive control drug and Pulsatilla saponin B4 were given 3 hours and 6 hours after modeling, and blood was collected from the eyeball after 3 hours of treatment. The whole blood taken from the mice was left at room temperature for 2 hours, centrifuged at 3500 rpm / min for 15 minutes, and 200 μl of the supernatant was taken to detect biochemical indicators related to kidney injury with a blood biochemical analyzer. Each mouse kidn...

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Abstract

The invention discloses an application of anemoside B4 used as an immunomodulator to drugs for treating acute inflammations. The anti-inflammatory effect of the anemoside B4 is directly produced through inhibition of lymphocyte activation and inflammatory factor secretion. The application of the anemoside B4 used as the immunomodulator to the drugs for treating acute inflammations is disclosed for the first time, and the anemoside B4 belongs to a purely natural preparation, is safe, reliable and free of side effects and has potential development value for the drugs for treating acute inflammations.

Description

technical field [0001] The invention relates to the application of Pulsatilla saponin B4 as an immunomodulator in the medicine for treating acute inflammation. Background technique [0002] Inflammation is the defense response of living tissue with vascular system to the stimulation of various damage factors. The typical response is redness, swelling, heat, pain and other clinical symptoms. Complex physiological and pathological responses. Inflammatory response is a protective defense response and a common pathway that causes many major human diseases, and is involved in many major diseases such as human infection, tumor, cardiovascular and cerebrovascular diseases, senile dementia, neurodegenerative diseases, allergic diseases, and mental illness occurrence and development process. Clinically, anti-inflammatory drugs are the second largest class of drugs after anti-infective drugs. [0003] Because the existing synthetic chemical anti-inflammatory drugs include non-stero...

Claims

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Application Information

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IPC IPC(8): A61K31/704A61P29/00A61P37/02A61P1/16A61P11/00A61P13/12
Inventor 刘琦
Owner 刘琦
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