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Application of uropolyacid peptide in preparation of medicine for treating scar

A uric acid peptide and scar technology, applied in the field of medicine, can solve the problems of easy recurrence and undiscovered uric acid peptide, etc., and achieve the effect of solving the insignificant curative effect.

Active Publication Date: 2019-04-12
泰凌(中国)投资有限公司 +4
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the clinical application of these drugs is limited due to their easy relapse and the inconvenience of long-term injection administration.
[0010] Have not yet found the report of the application of the uropolyacid peptide (CDA-II) for the preparation of the drug for the treatment of scar

Method used

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  • Application of uropolyacid peptide in preparation of medicine for treating scar
  • Application of uropolyacid peptide in preparation of medicine for treating scar
  • Application of uropolyacid peptide in preparation of medicine for treating scar

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Embodiment 1, preparation and identification of urinary polyacid peptide

[0040] 1. Preparation of Uric Polyacid Peptide Composition

[0041] Take 20L of fresh urine, add 1L of 1mol / l HCl to adjust its pH to 2.0, and filter the urine with nylon cloth. The ultrafiltration equipment performs ultrafiltration. Add deionized water to the ultrafiltration equipment, wash the ultrafiltration membrane, pour the collected urine into the feeding bucket, turn on the nanofiltration, and collect the filtrate with a molecular weight lower than 10kD.

[0042] Take 10kg of XAD-8 silica gel, soak it in 25L 95% ethanol for 20min, put it into a bag and place it in a plastic bucket to make an adsorption column, wash the column with 95% ethanol (V / W=ethanol / silica gel=2), and then use the same Wash the adsorption column twice with volume of deionized water to remove the ethanol in the column; then add the filtered urine to the column at a flow rate of 1.5l / min, and then add 20L distilled w...

experiment example 1

[0073] Experimental example 1 Urine polyacid peptide induces apoptosis of human skin hypertrophic scar fibroblasts (hHSF)

[0074] 1. Preparation of human skin hypertrophic scar fibroblasts

[0075] Take the skin hypertrophic scar of a patient aged 16-30, remove the epidermis and subcutaneous tissue under aseptic conditions, wash it repeatedly with normal saline, and cut it into 0.5-1.0mm 3 The tissue pieces were washed repeatedly with an appropriate amount of FBS (fetal calf serum), and then inoculated on the wall of a disposable culture bottle. The distance between the tissue pieces was controlled at 0.3-0.5mm, and 5ml of DMEM culture solution containing 20% ​​FBS (containing penicillin 100U / ml, streptomycin 100U / ml), at 37°C, 5% CO 2 Cultivate in the cell incubator, change the medium twice a week. When the primary cells grow and basically merge into a sheet, absorb the culture medium, rinse with PBS twice, add 0.25% trypsin to digest for 1min, and observe that the intercel...

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Abstract

The invention relates to the field of medicines, in particular to application of uroacitides and provides application of the uroacitides to preparation of cicatrix treatment medicines. The uroacitides can induce apoptosis of fibroblasts and inhibit proliferation of the fibroblasts, and fibroblast inhibition degree is increased along with increase of concentration of the uroacitides. According to experiments, fibroblast proliferation inhibition rate of the uroacitides is up to 88.45%, and skin cicatrices can be effectively treated by the uroacitides.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a new application of uropolyacid peptide in the preparation of medicine for treating skin scars. Background technique [0002] Uric acid peptide (CDA-II) is a non-cytotoxic biological agent composed of natural active ingredients isolated and purified from the fresh urine of healthy people. After analysis, it was found that its main ingredients are hippuric acid, phenylacetamide piperidine Diketones, phenylacetic acid, indole acetic acid, phenylacetylglutamine, indole acetic acid and small molecule peptides, etc. [0003] At present, uroacid peptide is mainly used in combination with chemotherapy, and can be used as adjuvant therapy for advanced breast cancer and non-small cell lung cancer. CN 99122049 discloses a urinary polyacid peptide composition and its use, which are mainly used for treating and preventing recurrence of cancer; CN200410000754.3 also relates to a urinary po...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/04A61K35/22A61P17/02A61K31/198A61K31/192A61K31/405
Inventor 陶俊张宝霞
Owner 泰凌(中国)投资有限公司
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