Chlorella active polypeptide powder preparing method
A technology of active polypeptide and chlorella, which is applied in protein food processing, food science, protein food ingredients, etc., can solve the problems of poor taste, fishy smell, low utilization rate of chlorella crude protein, etc., to achieve rich nutrition, Long shelf life, significant socially and economically significant effects
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Embodiment 1
[0018] The algal solution containing 500 mg / L live chlorella is separated by high-speed centrifugation, washed with deionized water, drained and treated with algae mud until the water content reaches 55%, and set aside. Mix 15g of corn steep liquor, 10g of soybean peptone, 25g of glucose, and 1L of water, adjust the pH to 7.0, sterilize at 0.1MPa for 20 minutes to obtain an expansion medium, inoculate Bacillus licheniformis on the expansion medium, and carry out 24h expansion culture , and then transfer the expanded Bacillus licheniformis into the obtained algae mud, the inoculum amount is 10wt%, the pH value is adjusted to 8.5, and the temperature is 40°C, and the shaker speed is 160rpm and cultivated for 10 days. After the fermentation is over, add deionized water to make the ratio of material to liquid in the fermentation broth reach 1:10, adjust the pH value to 8.5, add neutral protease 30U / g, lysozyme 20U / g, and at a temperature of 45°C, the enzyme Solution 8h. Spray-dry...
Embodiment 2
[0020] The algal solution containing 250 mg / L live chlorella is separated by high-speed centrifugation, washed with deionized water, drained and treated with algae mud until the water content reaches 45%, and set aside. Mix 10 g of corn steep liquor, 7 g of soybean peptone, 15 g of glucose, and 1 L of water, adjust the pH to 7.0, and sterilize at 0.1 MPa for 15 minutes to obtain an expansion medium, inoculate Bacillus licheniformis on the expansion medium, and carry out expansion culture for 18 hours , and then transfer the enlarged Bacillus licheniformis into the obtained algae mud, the inoculum amount is 8wt%, the pH value is adjusted to 6.5, and the temperature is 25°C, and the shaker speed is 120rpm. Cultivate for 6 days. After the fermentation is over, add deionized water to make the ratio of material to liquid in the fermentation broth reach 1:10, adjust the pH value to 6.8, add neutral protease 10U / g, lysozyme 10U / g, and at a temperature of 30°C, the enzyme Solution 7h....
Embodiment 3
[0022] The algal solution containing 400 mg / L live chlorella is separated by high-speed centrifugation, washed with deionized water, drained and treated with algae mud until the water content reaches 50%, and set aside. Mix 12.5g of corn steep liquor, 8.5g of soybean peptone, 20g of glucose, and 1L of water, adjust the pH to 7.0, and sterilize at 0.1MPa for 17 minutes to obtain an expanded medium, inoculate Bacillus licheniformis on the expanded medium, and carry out 20-hour incubation. Expand the culture, and then transfer the expanded Bacillus licheniformis into the obtained algae mud, the inoculum amount is 9wt%, the pH value is adjusted to 7.5, and the temperature is 37.5°C, and the shaker speed is 150rpm. Cultivate for 8 days. After the fermentation is over, add deionized water to make the ratio of material to liquid in the fermentation broth reach 1:10, adjust the pH value to 7.0, add 20 U / g of neutral protease and 15 U / g of lysozyme, and at a temperature of 37.5°C, the e...
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