Method for improving oil removing efficiency of oil removing bacteria

A kind of efficiency and seed technology, applied in the direction of microorganism-based methods, chemical instruments and methods, biochemical equipment and methods, etc., can solve the problems of reduced oil removal efficiency and the influence of environmental factors, and achieve good results, small magnetic field strength, The effect of enhancing resistance

Active Publication Date: 2016-01-20
HARBIN ENG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The key to microbial oil removal technology lies in biological strains and biological treatment processes. According to the particularity of oily wastewater, the development of efficient biological strains and efficient treatment processes is a research hotspot in this field. However, the existing microbial methods to treat wastewater containing Susceptible to environmental factors, resulting in reduced oil removal efficiency

Method used

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  • Method for improving oil removing efficiency of oil removing bacteria
  • Method for improving oil removing efficiency of oil removing bacteria
  • Method for improving oil removing efficiency of oil removing bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Embodiment 1: the influence of magnetic field on the growth of degreasing bacteria

[0050] Take the Acinetobactersp.B11 bacteria stored on the slant of the culture medium at 4°C and spread it on the nutrient agar medium plate. After culturing at 30°C for 24-36 hours, pick a single colony and inoculate it into the seed liquid medium, shake it at 30°C and 200r / min Cultivate for 12h. Measure the concentration of the bacterial suspension at OD600 wavelength and dilute the bacterial suspension to 0.8, and use it as a seed solution. According to the inoculation amount of 5%, the strain seed solution was inoculated into the degradation medium of 200mg / L petroleum concentration. Add magnetic fields of 0, 18, 25, 35, and 60mT respectively, and use the blank culture medium without inoculation as a control, and culture it on a shaker at 30°C and 100r / min for 7 days. The results are shown in figure 2 . Depend on figure 2 The experimental results show that the adaptability of...

Embodiment 2

[0051] Embodiment 2: the influence of magnetic field strength on degreasing effectiveness of degreasing bacteria

[0052] Take the Acinetobactersp.B11 bacteria stored on the slant of the culture medium at 4°C and spread it on the nutrient agar medium plate. After culturing at 30°C for 24-36 hours, pick a single colony and inoculate it into the seed liquid medium, shake it at 30°C and 200r / min Cultivate for 12h. Measure the concentration of the bacterial suspension at OD600 wavelength and dilute the bacterial suspension to 0.8, and use it as a seed solution. According to the inoculation amount of 5%, the strain seed solution was inoculated into the degradation medium of 200mg / L petroleum concentration. Magnetic fields of 0, 18, 25, 35, and 60 mT were applied respectively, and a blank culture medium without inoculation was used as a control, and cultured on a shaking table at 30° C. and 100 r / min for 7 days. The effect of microorganisms on the oil degradation efficiency under ...

Embodiment 3

[0053] Embodiment 3: the influence of magnetic field on anti-shock load of degreasing bacteria

[0054] After activation, the oil-removing bacteria were inoculated into fresh liquid petroleum medium, and cultured on a shaker at 30°C and 100r / min for 7 days under the condition of 25mT magnetic field strength and no magnetic field, and the initial oil concentrations were 100, 200 , 500, 1000, 1500, 2000, 3000mg / L. The oil degradation rate and biomass were measured respectively to investigate the effect of initial oil concentration on microbial oil degradation rate and the strengthening effect of magnetic field on microbial oil removal rate at different initial oil concentrations, and determine the most suitable microbial oil degradation concentration. The degradation results of microorganisms under different initial oil concentrations are shown in Figure 4 . The microbial degradation rate of oil showed a trend of first increasing and then decreasing with the increase of initi...

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Abstract

The invention aims to provide a method for improving oil removing efficiency of oil removing bacteria. According to the microorganism oil removing method, a magnetic field is additionally arranged around the oil removing bacteria, in the mode of adjusting the intensity and action of the magnetic field, the degrading effect on petroleum and the capacity for resisting impact loads of the oil removing bacteria can be effectively improved, process operation stability is improved, and the method belongs to the field of biological treatment on oily sewage. An adopted permanent magnet is a round ferrite permanent magnet, according to the action condition, the magnetic field intensity is 18-35 mT, and the magnetic action is continuous. The magnetic field is generated by the commercially available permanent magnet, the needed magnetic field intensity is low, electric energy consumption is not needed, investment is low, the effect is good, an existing process flow path is not changed, arrangement of large equipment is avoided, and the enhanced oily sewage treatment technology is suitable for the situation of China, economical and feasible.

Description

technical field [0001] The invention relates to a biological treatment method for oily sewage, in particular to a method for enhancing the degreasing efficiency by strengthening the degreasing bacteria with a magnetic field. Background technique [0002] As the main energy source of industrial civilization, oil affects many aspects of social development. People call oil "the blood of industry". During the development, transportation, processing and storage of petroleum, a large amount of petroleum and its products enter the environment, causing serious petroleum pollution. Oil pollutes surface water, destroys the growth of aquatic organisms, and threatens the safety of water supply; pollutes soil, destroys soil structure, hinders the normal growth of plants, and enters the human body through crop enrichment, endangering human health; pollutes the ocean, affects the entry of light and oxygen, and destroys marine ecology Balance, etc., brings a series of serious economic and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34C12N1/20C12R1/01
Inventor 任芝军官涤施悦刘迁张志柳
Owner HARBIN ENG UNIV
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