Screening method of resisting joint toxicity of heavy metals by trisodium citrate dihydrate

A technology of citric acid dihydrate and combined toxicity, applied in biological testing, material inspection products, measuring devices, etc., can solve problems such as oxidative stress damage of cell tissues, and achieve fast screening, low cost, and high screening efficiency Effect

Active Publication Date: 2016-01-20
NINGBO UNIV
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Problems solved by technology

In addition, studies have shown that almost all heavy metal ions can cause the generation of active oxygen groups in the body, which in turn leads to oxidative stress damage in cells and tissues

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  • Screening method of resisting joint toxicity of heavy metals by trisodium citrate dihydrate
  • Screening method of resisting joint toxicity of heavy metals by trisodium citrate dihydrate
  • Screening method of resisting joint toxicity of heavy metals by trisodium citrate dihydrate

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Embodiment Construction

[0028] The present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments.

[0029] A screening method for antagonizing the combined toxicity of heavy metal pollutants with trisodium citrate dihydrate, comprising the following steps:

[0030] Step 1, configuring a mixed solution of various heavy metal pollutants;

[0031] Step 2, determine the LC50 of the heavy metal mixed solution by MTT experiment;

[0032] Step 3: Using trisodium citrate dihydrate as an antagonist, the aqueous solution of trisodium citrate dihydrate of different concentrations to be screened and the mixed solution of heavy metals at half the lethal concentration of cells are mixed in turn, and added to the culture medium for cell line culture ;

[0033] Step 4, MTT cell viability test: exposure to the virus for 24 hours, followed by MTT incubation for 3 to 4 hours; cell viability was measured to obtain the antagonism efficiency of different concen...

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Abstract

The invention discloses a screening method of resisting joint toxicity of heavy metals by trisodium citrate dehydrate. The screening method comprises the following steps: step one, preparing a mixed solution of multiple heavy metal pollutants; step two, determining LC50 of the heavy metal mixed solution by MTT; step three, by taking trisodium citrate dehydrate as an antagonist, sequentially mixing trisodium citrate dehydrate water solutions with different concentrations to be screened and the heavy metal mixed solution with concentration capable of causing the death of half number of cells, adding the mixture into a culture medium to be used as a cell line to be cultured; and step four, testing the survival activity of MTT cells, namely, poisoning for 24h, adding MTT, incubating for 3-4h, and measuring the survival activity of the cells, thus obtaining the resisting efficiency of the chemical antagonists with different concentrations to be screened. The screening method has the advantages that the screening rapid is high, the cost is low, and the antagonists with different concentrations can be screened simultaneously, so that the screening efficiency is high.

Description

technical field [0001] The invention relates to the technical field of heavy metal pollutant repellent, in particular to the repellent combined toxicity of various heavy metal pollutants, in particular to a screening method for trisodium citrate dihydrate to antagonize the combined toxicity of heavy metal pollutants. Background technique [0002] Heavy metals generally refer to metal elements with a specific gravity greater than 4.0. Heavy metals or their compounds are a class of pollutants that are difficult to degrade in the environment. They are widely present in the atmosphere, water, soil and other environmental media and cause serious environmental pollution. Heavy metal pollution refers to environmental pollution caused by heavy metals or their compounds. In recent years, there have been frequent incidents of heavy metal pollution in my country, ranging from excessive blood lead in children to heavy metal pollution of "cadmium rice" and so on. Heavy metal pollution ha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/50
CPCG01N33/5014
Inventor 苏红赵进顺王亚飞谷元亮邹宝波
Owner NINGBO UNIV
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