Applications of gentiana macrophylla pall pill prescription in preparation of anti-aging drugs
A Qin Gentiana Pill, anti-aging technology, applied in the field of traditional Chinese medicine, can solve the problems of short life cycle, few anti-aging drugs, unclear function and curative effect of drugs, etc.
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Embodiment 1
[0035] Embodiment 1 Preparation of the water extract of Chinese medicine compound Qinjie pill
[0036] Zanthoxylum bungeanum 3g, Rehmannia glutinosa 3g, Roasted Inula 3g, Angelica 3g. Soak in distilled water for 20 minutes, then simmer and boil for 30 minutes, dilute the final volume to 100 mL, centrifuge, discard the precipitate, sterile filter the supernatant, and store it in a 4°C refrigerator for later use.
Embodiment 2
[0037] Example 2 Effects of traditional Chinese medicine compound Qinjie Wan on the lifespan of mutant progeria TK22 Caenorhabditis elegans
[0038] 1. Caenorhabditis elegans synchronization
[0039] The hatched adult worms of TK22 strain Caenorhabditis elegans were washed with M9 buffer into a 1.5mL centrifuge tube, left standing or centrifuged and discarded. Add 500μL of 1M NaOH and 500μL of 6.4% NaClO to each centrifuge tube, and put them in a vortexer. Lysis by shaking for about 7 min, centrifuged at 4000 rpm for 4 min, aspirate the supernatant, rinse twice with M9, add 500 μL of M9, incubate in a 20°C incubator, and inoculate it in an NGM solid Petri dish coated with OP50. Synchronized growth of C. elegans can be obtained by culturing at 20°C.
[0040] 2. Preparation of NGM plate containing Chinese medicine compound Qinjie pills
[0041] The extract of the Chinese medicine compound Qinqu Pill prepared in Example 1 is prepared according to the following method:
[0042]...
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