A kind of preparation method of high-strength polypeptide hydrogel

A hydrogel, high-strength technology, applied in the direction of non-active ingredient medical preparations, medical preparations containing active ingredients, pharmaceutical formulations, etc., can solve the problem of low cross-linking strength, poor self-supporting performance, difficult to effectively control, etc. problem, to achieve the effect of improving mechanical strength and gelling ability

Active Publication Date: 2017-12-15
CHINA UNIV OF PETROLEUM (EAST CHINA)
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This process is based on the formation of non-covalent interactions, the ability to form gels is limited, and the cross-linking strength between fibers is often low, resulting in limited gel strength, poor self-supporting performance, and easy to be destroyed; moreover, its fiber cross-linking The pore size of the three-dimensional network structure formed by the association is often small and difficult to effectively control, which greatly limits its application in the field of tissue engineering materials.

Method used

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  • A kind of preparation method of high-strength polypeptide hydrogel
  • A kind of preparation method of high-strength polypeptide hydrogel
  • A kind of preparation method of high-strength polypeptide hydrogel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] (1) Preparation I 3 K polypeptide molecule 4.0mmol / L aqueous solution, ultrasonic mixing, ultrasonic power 100W, ultrasonic time 10min; stand at room temperature for 3 days to make it self-assemble into a long fiber structure, at this time the system cannot form a hydrogel;

[0036] (2) Add GO sheets with a length of 300±200nm, a width of 300±200nm, and a thickness of 1-2nm in the solution containing the long polypeptide fiber structure, so that the concentration of the GO sheet is 0.1mg / mL (that is, every mL Add 0.1mg GO flakes to the solution), mix with ultrasonic, the ultrasonic power is 100W, and the ultrasonic time is 12min; the pH value of the mixed solution is adjusted to 10.5 by using NaOH solution with a concentration of 0.5mol / L, and it is allowed to stand at room temperature for 6 hours to form a hybrid Hydrogels.

[0037]The storage modulus (G') of the hydrogel obtained in Example 1 can reach 4000Pa, the energy dissipation modulus (G") can reach 500Pa, and ...

Embodiment 2

[0039] (1) Prepare K 3 A 3 I 3 G 3 V 3 Polypeptide molecule 4.0mmol / L aqueous solution, ultrasonic mixing, ultrasonic power 100W, ultrasonic time 10min; stand at room temperature for 3 days to make it self-assemble into a long fiber structure;

[0040] (2) Add GO sheets with a length of 300±200nm, a width of 300±200nm, and a thickness of 1-2nm to the above-mentioned long fiber structure solution, so that the concentration of the GO sheet is 0.02mg / mL, ultrasonically mix, and the ultrasonic power 100W, ultrasonic time is 10min; use 0.5mol / L NaOH solution to adjust the pH of the mixed solution to 10.0, and let it stand at room temperature for 6 hours to form a hybrid hydrogel.

[0041] The storage modulus (G') of the hydrogel obtained in Example 2 can reach 5000Pa, the energy dissipation modulus (G") can reach 600Pa, and the gel pore size is less than 500nm; the self-supporting hybrid Hydrogels.

Embodiment 3

[0043] (1) Prepare K 3 A 3 I 3 G 3 V 3 Polypeptide molecule 4.0mmol / L aqueous solution, ultrasonic mixing, ultrasonic power 100W, ultrasonic time 10min; stand at room temperature for 3 days to make it self-assemble into a long fiber structure;

[0044] (2) Add GO sheets with a length of 300±200nm, a width of 300±200nm, and a thickness of 1-2nm to the above-mentioned long fiber structure solution, so that the concentration of the GO sheet is 0.1mg / mL, ultrasonically mix, and the ultrasonic power 100W, ultrasonic time is 10min; use 0.5mol / L NaOH solution to adjust the pH of the mixed solution to 10.0, and let it stand at room temperature for 6 hours to form a hybrid hydrogel.

[0045] The storage modulus (G') of the hydrogel obtained in Example 3 can reach 6000Pa, the loss modulus (G") can reach 1000Pa, and the gel pore size is less than 500nm. figure 2 It is the rheological measurement result of the hybrid hydrogel of this embodiment; a self-supporting hybrid hydrogel can...

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Abstract

The invention relates to a preparation method of high-strength polypeptide hydrogel, which belongs to the field of chemical preparation. The preparation method includes: (1) selecting a positively charged polypeptide molecule capable of self-assembling to form a long fiber structure, preparing its aqueous solution, ultrasonically mixing, and standing still to make it self-assemble into a long fiber structure; (2) forming a long fiber structure Graphene oxide (GO) sheets were added to the solution, ultrasonically mixed, the pH value adjusted, and left to stand until the system matured and stabilized to form a hybrid hydrogel. The present invention introduces GO sheets into the polypeptide hydrogel, improves the gelation ability of the system, and obtains high-strength polypeptide / GO hybrid hydrogel materials; it can be controlled by the size, surface charge density and concentration of GO sheets To regulate the mechanical strength and pore size of the polypeptide / GO hybrid hydrogel.

Description

technical field [0001] The invention relates to a preparation method of a hydrogel, in particular to a preparation method of a high-strength polypeptide hydrogel; it belongs to the field of chemical preparation. Background technique [0002] Hydrogel is a gel material with water as the dispersion medium. Due to its rich pore structure and three-dimensional network structure that can provide better permeability and mechanical support, it has broad application prospects in many fields. In recent years, hydrogel materials have attracted the attention of scientists at home and abroad, and a lot of research has been done on their applications in biomedicine, such as tissue engineering and drug delivery. Ordinary hydrogels can be obtained by covalent cross-linking between high-molecular-weight synthetic polymers or between some small molecules, but such cross-linking methods need to be carried out by chemical synthesis. Since most of the chemical reagents are toxic, the hydrogel ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/02A61L27/24A61L27/08A61L27/52A61L27/56A61K9/06A61K47/42A61K47/04
Inventor 曹美文徐海汪蕾王继乾王栋吕建仁
Owner CHINA UNIV OF PETROLEUM (EAST CHINA)
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